LOCUS       sequence-242278-        5046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for anti-sense in situ probe in vitro transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Castro JP, Yancoskie MN, Marchini M, Belohlavy S, Hiramatsu L, Kucka
            M, Beluch WH, Naumann R, Skuplik I, Cobb J, Barton NH, Rolian C, 
            Chan YF
  TITLE     An integrative genomic analysis of the Longshanks selection 
            experiment for longer limbs in mice.
  JOURNAL   Elife. 2019 Jun 6;8:e42014. doi: 10.7554/eLife.42014.
  PUBMED    31169497
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-06-6"; volume: "8:e42014. doi"; pages: " 
            10.7554/eLife.42014"
COMMENT     Sequence Label: pJET1.2_Bod1l_Anti-Sense
FEATURES             Location/Qualifiers
     source          1..5046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(1375..1381,1382..1399,1400..1405))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(2760..2777)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2931..3519)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3011..3030)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3690..4481,4482..4550))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4313..4332
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4551..4655)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4723..4741
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 taatacgact cactataggg agagcggccg ccagatcttc cggatggctc gagtttttca
       61 gcaagatcac tgtgagttcg tcatcagaat cactgtagta ctttgagtaa agatacggtt
      121 tgtgaacata agcatgccgt acgcctctgg cttttccatc actagaatca ccaggttgaa
      181 cttttgcttt attctgcttg tttttctctt catcctctga tgtaatctct ccttcttcca
      241 tgctctcaga cacgacaact tcctcctctg tgtcttcttc aaaggacgaa aggtcactgg
      301 tgtggacaga gctgactgtg atatcagtga gaccatccac atcagaatct aacaaagaac
      361 aatcttcttt tgtcccctca gcagccctgg ctctgctgtt gctcttttca gaaggaagct
      421 ttgtgctctc cacttctttg tcctttgctt gtctctcatc tttcactttc tgtgtatctt
      481 cattcctttt agagtgatca attttttttt cagtcttttc cttcttctct ttctttctct
      541 ctcctttctc attaccatcg ggtttctttt ctcctttgtc cattgattta atttttgggt
      601 cagtgctttc ctgctgagca tccttactta gtaacagtaa gctattattc tctttcatat
      661 gatttttagt ttcttccact ggacaaggga gatcactggg ttcttctgac ttgagagcag
      721 cttctagccc atctcctcca gaatcagagg tagccttttc tctctcagtc gcatcttctg
      781 agcctctctc cttgtctgtg ctaacgtcag tgcttggctg agatgagagt tttctggatg
      841 ttctctcact gaccttggca tttgacattt ctgttgaagc tctggcagcg ttagcttctt
      901 ggttaagaga agttatggtt tccaagattg acatggcatc atctttctag aagatctcct
      961 acaatattct cagctgccat ggaaaatcga tgttcttctt ttattctctc aagattttca
     1021 ggctgtatat taaaacttat attaagaact atgctaacca cctcatcagg aaccgttgta
     1081 ggtggcgtgg gttttcttgg caatcgactc tcatgaaaac tacgagctaa atattcaata
     1141 tgttcctctt gaccaacttt attctgcatt ttttttgaac gaggtttaga gcaagcttca
     1201 ggaaactgag acaggaattt tattaaaaat ttaaattttg aagaaagttc agggttaata
     1261 gcatccattt tttgctttgc aagttcctca gcattcttaa caaaagacgt ctcttttgac
     1321 atgtttaaag tttaaacctc ctgtgtgaaa ttattatccg ctcataattc cacacattat
     1381 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aaggaaggtg
     1441 cgaacaagtc cctgatatga gatcatgttt gtcatctgga gccatagaac agggttcatc
     1501 atgagtcatc aacttacctt cgccgacagt gaattcagca gtaagcgccg tcagaccaga
     1561 aaagagattt tcttgtcccg catggagcag attctgccat ggcaaaacat ggtggaagtc
     1621 atcgagccgt tttaccccaa ggctggtaat ggccggcgac cttatccgct ggaaaccatg
     1681 ctacgcattc actgcatgca gcattggtac aacctgagcg atggcgcgat ggaagatgct
     1741 ctgtacgaaa tcgcctccat gcgtctgttt gcccggttat ccctggatag cgccttgccg
     1801 gaccgcacca ccatcatgaa tttccgccac ctgctggagc agcatcaact ggcccgccaa
     1861 ttgttcaaga ccatcaatcg ctggctggcc gaagcaggcg tcatgatgac tcaaggcacc
     1921 ttggtcgatg ccaccatcat tgaggcaccc agctcgacca agaacaaaga gcagcaacgc
     1981 gatccggaga tgcatcagac caagaaaggc aatcagtggc actttggcat gaaggcccac
     2041 attggtgtcg atgccaagag tggcctgacc cacagcctgg tcaccaccgc ggccaacgag
     2101 catgacctca atcagctggg taatctgctg catggagagg agcaatttgt ctcagccgat
     2161 gccggctacc aaggggcgcc acagcgcgag gagctggccg aggtggatgt ggactggctg
     2221 atcgccgagc gccccggcaa ggtaagaacc ttgaaacagc atccacgcaa gaacaaaacg
     2281 gccatcaaca tcgaatacat gaaagccagc atccgggcca gggtggagca cccatttcgc
     2341 atcatcaagc gacagttcgg cttcgtgaaa gccagataca aggggttgct gaaaaacgat
     2401 aaccaactgg cgatgttatt cacgctggcc aacctgtttc gggcggacca aatgatacgt
     2461 cagtgggaga gatctcacta aaaactgggg ataacgcctt aaatggcgaa gaaacggtct
     2521 aaataggctg attcaaggca tttacgggag aaaaaatcgg ctcaaacatg aagaaatgaa
     2581 atgactgagt cagccgagaa gaatttcccc gcttattcgc accttcccta actcacatta
     2641 attgcgttgc gctcactgcc aattgctttc cagtcgggaa acctgtcgtg ccagctgcat
     2701 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     2761 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2821 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2881 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2941 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3001 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3061 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3121 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3181 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3241 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3301 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3361 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3421 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3481 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3541 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3601 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3661 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3721 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3781 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3841 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3901 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3961 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4021 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4081 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4141 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4201 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4261 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4321 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4381 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4441 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4501 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4561 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4621 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4681 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     4741 ccgcccctgc agccgaatta tattattttt gccaaataat ttttaacaaa agctctgaag
     4801 tcttcttcat ttaaattctt agatgatact tcatctggaa aattgtccca attagtagca
     4861 tcacgctgtg agtaagttct aaaccatttt tttattgttg tattatctct aatcttacta
     4921 ctcgatgagt tttcggtatt atctctattt ttaacttgga gcaggttcca ttcattgttt
     4981 ttttcatcat agtgaataaa atcaactgct ttaacacttg tgcctgaaca ccatatccat
     5041 ccggcg
//
