LOCUS       sequence-242287-        8856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of wild-type catalytic domain only form of TET1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8856)
  AUTHORS   Vincenzetti L, Leoni C, Chirichella M, Kwee I, Monticelli S
  TITLE     The contribution of active and passive mechanisms of 5mC and 5hmC 
            removal in human T lymphocytes is differentiation- and 
            activation-dependent.
  JOURNAL   Eur J Immunol. 2019 Jan 30. doi: 10.1002/eji.201847967.
  PUBMED    30698829
REFERENCE   2  (bases 1 to 8856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J 
            Immunol. 2019 Jan 30. doi: 10.1002/eji.201847967."
COMMENT     Sequence Label: pEF1a_CD WT TET1
FEATURES             Location/Qualifiers
     source          1..8856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(45..64)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        352..1529
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          583..1520
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1477..1497
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1546..1565
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1546..1564
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1597..1606
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1606..1629
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1642..1668
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             1642..1668
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4366..4407
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4417..4434
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4457..4474)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4463..4687
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4733..5161
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4820..4839)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5030..5051
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5170..5190)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5175..5504
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5355..5490
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5417..5436
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5571..6365
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5625..5644)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6235..6254
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6541..6662
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6578..6597)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6632..6651
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6711..6727)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6711..6727)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6724..6746)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6735..6751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6759..6765,6766..6783,6784..6789))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6804..6825
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6942..6959)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7102..7690)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7182..7201)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7861..8652,8653..8721))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8484..8503
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8722..8826)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctaggctttt gcaaaaagct ttgcaaagat ggataaagtt ttaaacagag
      301 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt
      361 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
      421 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
      481 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
      541 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
      601 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
      661 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
      721 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
      781 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
      841 tctctagcca tttaaaattt ttgatgacct gctgcgacgc ttttttctgg caagatagtc
      901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
      961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
     1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
     1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta
     1561 tagggagacc caagctggct aggtaagctt ggtaccgcca ccatggacta caaggacgac
     1621 gatgacaagc tcgatggagg atacccctac gacgtgcccg actacgccgg aggatatcct
     1681 gaatcagcag aggaaaaggt gaaggttgaa ccattggatt cactcagctt atttcatctt
     1741 aaaacggaat ccaacgggaa ggcattcact gataaagctt ataattctca ggtacagtta
     1801 acggtgaatg ccaatcagaa agcccatcct ttgacccagc cctcctctcc acctaaccag
     1861 tgtgctaacg tgatggcagg cgatgaccaa atacggtttc agcaggttgt taaggagcaa
     1921 ctcatgcatc agagactgcc aacattgcct ggtatctctc atgaaacacc cttaccggag
     1981 tcagcactaa ctctcaggaa tgtaaatgta gtgtgttcag gtggaattac agtggtttct
     2041 accaaaagtg aagaggaagt ctgttcatcc agttttggaa catcagaatt ttccacagtg
     2101 gacagtgcac agaaaaattt taatgattat gccatgaact tctttactaa ccctacaaaa
     2161 aacctagtgt ctataactaa agattctgaa ctgcccacct gcagctgtct tgatcgagtt
     2221 atacaaaaag acaaaggccc atattataca caccttgggg caggaccaag tgttgctgct
     2281 gtcagggaaa tcatggagaa taggtatggt caaaaaggaa acgcaataag gatagaaata
     2341 gtagtgtaca ccggtaaaga agggaaaagc tctcatgggt gtccaattgc taagtgggtt
     2401 ttaagaagaa gcagtgatga agaaaaagtt ctttgtttgg tccggcagcg tacaggccac
     2461 cactgtccaa ctgctgtgat ggtggtgctc atcatggtgt gggatggcat ccctcttcca
     2521 atggccgacc ggctatacac agagctcaca gagaatctaa agtcatacaa tgggcaccct
     2581 accgacagaa gatgcaccct caatgaaaat cgtacctgta catgtcaagg aattgatcca
     2641 gagacttgtg gagcttcatt ctcttttggc tgttcatgga gtatgtactt taatggctgt
     2701 aagtttggta gaagcccaag ccccagaaga tttagaattg atccaagctc tcccttacat
     2761 gaaaaaaacc ttgaagataa cttacagagt ttggctacac gattagctcc aatttataag
     2821 cagtatgctc cagtagctta ccaaaatcag gtggaatatg aaaatgttgc ccgagaatgt
     2881 cggcttggca gcaaggaagg tcgtcccttc tctggggtca ctgcttgcct ggacttctgt
     2941 gctcatcccc acagggacat tcacaacatg aataatggaa gcactgtggt ttgtacctta
     3001 actcgagaag ataaccgctc tttgggtgtt attcctcaag atgagcagct ccatgtgcta
     3061 cctctttata agctttcaga cacagatgag tttggctcca aggaaggaat ggaagccaag
     3121 atcaaatctg gggccatcga ggtcctggca ccccgccgca aaaaaagaac gtgtttcact
     3181 cagcctgttc cccgttctgg aaagaagagg gctgcgatga tgacagaggt tcttgcacat
     3241 aagataaggg cagtggaaaa gaaacctatt ccccgaatca agcggaagaa taactcaaca
     3301 acaacaaaca acagtaagcc ttcgtcactg ccaaccttag ggagtaacac tgagaccgtg
     3361 caacctgaag taaaaagtga aaccgaaccc cattttatct taaaaagttc agacaacact
     3421 aaaacttatt cgctgatgcc atccgctcct cacccagtga aagaggcatc tccaggcttc
     3481 tcctggtccc cgaagactgc ttcagccaca ccagctccac tgaagaatga cgcaacagcc
     3541 tcatgcgggt tttcagaaag aagcagcact ccccactgta cgatgccttc gggaagactc
     3601 agtggtgcca atgcagctgc tgctgatggc cctggcattt cacagcttgg cgaagtggct
     3661 cctctcccca ccctgtctgc tcctgtgatg gagcccctca ttaattctga gccttccact
     3721 ggtgtgactg agccgctaac gcctcatcag ccaaaccacc agccctcctt cctcacctct
     3781 cctcaagacc ttgcctcttc tccaatggaa gaagatgagc agcattctga agcagatgag
     3841 cctccatcag acgaacccct atctgatgac cccctgtcac ctgctgagga gaaattgccc
     3901 cacattgatg agtattggtc agacagtgag cacatctttt tggatgcaaa tattggtggg
     3961 gtggccatcg cacctgctca cggctcggtt ttgattgagt gtgcccggcg agagctgcac
     4021 gctaccactc ctgttgagca ccccaaccgt aatcatccaa cccgcctctc ccttgtcttt
     4081 taccagcaca aaaacctaaa taagccccaa catggttttg aactaaacaa gattaagttt
     4141 gaggctaaag aagctaagaa taagaaaatg aaggcctcag agcaaaaaga ccaggcagct
     4201 aatgaaggtc cagaacagtc ctctgaagta aatgaattga accaaattcc ttctcataaa
     4261 gcattaacat taacccatga caatgttgtc accgtgtccc cttatgctct cacacacgtt
     4321 gcggggccct ataaccattg ggtctgatct agagggccct tcgaaggtaa gcctatccct
     4381 aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca ccattgagtt
     4441 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     4501 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     4561 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     4621 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     4681 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     4741 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4801 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4861 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4921 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4981 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5041 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5101 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5161 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     5221 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     5281 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     5341 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     5401 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     5461 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     5521 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     5581 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     5641 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     5701 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     5761 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     5821 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     5881 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     5941 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     6001 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     6061 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     6121 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     6181 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     6241 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     6301 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     6361 tctgagcggg actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca
     6421 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     6481 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     6541 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6601 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6661 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     6721 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6781 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6841 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6901 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6961 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7021 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca ggaaccgtaa
     7081 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7141 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7201 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7261 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7321 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7381 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7441 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7501 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7561 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7621 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7681 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7741 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7801 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7861 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7921 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7981 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8041 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8101 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8161 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     8221 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     8281 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8341 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8401 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8461 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8521 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8581 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8641 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8701 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8761 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8821 tccgcgcaca tttccccgaa aagtgccacc tgacgt
//
