LOCUS       sequence-242293-       10550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10550)
  AUTHORS   Liu P, Luk K, Shin M, Idrizi F, Kwok S, Roscoe B, Mintzer E, Suresh 
            S, Morrison K, Frazao JB, Bolukbasi MF, Ponnienselvan K, Luban J, 
            Zhu LJ, Lawson ND, Wolfe SA
  TITLE     Enhanced Cas12a editing in mammalian cells and zebrafish.
  JOURNAL   Nucleic Acids Res. 2019 Mar 20. pii: 5403491. doi: 
            10.1093/nar/gkz184.
  PUBMED    30892626
REFERENCE   2  (bases 1 to 10550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2019 Mar 20. pii: 5403491. doi: 10.1093/nar/gkz184."
COMMENT     Sequence Label: pET-21a_2xNLS_FnCpf1_MBP_protein_expression
FEATURES             Location/Qualifiers
     source          1..10550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     570..589
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(609..686)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     892..911
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     995..1014
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        995..1013
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1014..1038
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1053..1075
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4983..5030
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5037..5117
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5118..5138
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5148..5168
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             5169..6269
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     6243..6266
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             6276..6293
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6346..6364)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6360..6407
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6444..6899
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6531..6550)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6741..6762
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6925..7029
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7030..7098,7099..7890)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7248..7267)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8061..8649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8550..8569
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8803..8820
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8835..8974
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8900..8919)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9060..9082
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(9076..9267)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(10076..10550,1..608))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc
       61 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca
      121 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga
      181 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg
      241 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg
      301 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta
      361 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc
      421 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc
      481 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc
      541 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact
      601 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga
      661 aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg
      721 cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg
      781 tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
      841 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc
      901 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc
      961 ggcgtagagg atcgagatct cgatcccgcg aaattaatac gactcactat aggggaattg
     1021 tgagcggata acaattcccc tctagaaata attttgttta actttaagaa ggagatatac
     1081 atatgagcat ctaccaggag ttcgtcaaca agtattcact gagtaagaca ctgcggttcg
     1141 agctgatccc acagggcaag acactggaga acatcaaggc ccgaggcctg attctggacg
     1201 atgagaagcg ggcaaaagac tataagaaag ccaagcagat cattgataaa taccaccagt
     1261 tctttatcga ggaaattctg agctccgtgt gcatcagtga ggatctgctg cagaattact
     1321 cagacgtgta cttcaagctg aagaagagcg acgatgacaa cctgcagaag gacttcaagt
     1381 ccgccaagga caccatcaag aaacagatta gcgagtacat caaggactcc gaaaagttta
     1441 aaaatctgtt caaccagaat ctgatcgatg ctaagaaagg ccaggagtcc gacctgatcc
     1501 tgtggctgaa acagtctaag gacaatggga ttgaactgtt caaggctaac tccgatatca
     1561 ctgatattga cgaggcactg gaaatcatca agagcttcaa gggatggacc acatacttta
     1621 aaggcttcca cgagaaccgc aagaacgtgt actccagcaa cgacattcct acctccatca
     1681 tctaccgaat cgtcgatgac aatctgccaa agttcctgga gaacaaggcc aaatatgaat
     1741 ctctgaagga caaagctccc gaggcaatta attacgaaca gatcaagaaa gatctggctg
     1801 aggaactgac attcgatatc gactataaga ctagcgaggt gaaccagagg gtcttttccc
     1861 tggacgaggt gtttgaaatc gccaatttca acaattacct gaaccagtcc ggcattacta
     1921 aattcaatac catcattggc gggaagtttg tgaacgggga gaataccaag cgcaagggaa
     1981 ttaacgaata catcaatctg tatagccagc agatcaacga caaaactctg aagaaataca
     2041 agatgtctgt gctgttcaaa cagatcctga gtgataccga gtccaagtct tttgtcattg
     2101 ataaactgga agatgactca gacgtggtca ctaccatgca gagcttttat gagcagatcg
     2161 ccgctttcaa gacagtggag gaaaaatcta ttaaggaaac tctgagtctg ctgttcgatg
     2221 acctgaaagc ccagaagctg gacctgagta agatctactt caaaaacgat aagagtctga
     2281 cagacctgtc acagcaggtg tttgatgact attccgtgat tgggaccgcc gtcctggagt
     2341 acattacaca gcagatcgct ccaaagaacc tggataatcc ctctaagaaa gagcaggaac
     2401 tgatcgctaa gaaaaccgag aaggcaaaat atctgagtct ggaaacaatt aagctggcac
     2461 tggaggagtt caacaagcac agggatattg acaaacagtg ccgctttgag gaaatcctgg
     2521 ccaacttcgc agccatcccc atgatttttg atgagatcgc ccagaacaaa gacaatctgg
     2581 ctcagatcag tattaagtac cagaaccagg gcaagaaaga cctgctgcag gcttcagcag
     2641 aagatgacgt gaaagccatc aaggatctgc tggaccagac caacaatctg ctgcacaagc
     2701 tgaaaatctt ccatattagt cagtcagagg ataaggctaa tatcctggat aaagacgaac
     2761 acttctacct ggtgttcgag gaatgttact tcgagctggc aaacattgtc cccctgtata
     2821 acaagattag gaactacatc acacagaagc cttactctga cgagaagttt aaactgaact
     2881 tcgaaaatag taccctggcc aacgggtggg ataagaacaa ggagcctgac aacacagcta
     2941 tcctgttcat caaggatgac aagtactatc tgggagtgat gaataagaaa aacaataaga
     3001 tcttcgatga caaagccatt aaggagaaca aaggggaagg atacaagaaa atcgtgtata
     3061 agctgctgcc cggcgcaaat aagatgctgc ctaaggtgtt cttcagcgcc aagagtatca
     3121 aattctacaa cccatccgag gacatcctgc ggattagaaa tcactcaaca catactaaga
     3181 acgggagccc ccagaaggga tatgagaaat ttgagttcaa catcgaggat tgcaggaagt
     3241 ttattgactt ctacaagcag agcatctcca aacaccctga atggaaggat tttggcttcc
     3301 ggttttccga cacacagaga tataactcta tcgacgagtt ctaccgcgag gtggaaaatc
     3361 aggggtataa gctgactttt gagaacattt ctgaaagtta catcgacagc gtggtcaatc
     3421 agggaaagct gtacctgttc cagatctata acaaagattt ttcagcatac agcaagggca
     3481 gaccaaacct gcatacactg tactggaagg ccctgttcga tgagaggaat ctgcaggacg
     3541 tggtctataa actgaacgga gaggccgaac tgttttaccg gaagcagtct attcctaaga
     3601 aaatcactca cccagctaag gaggccatcg ctaacaagaa caaggacaat cctaagaaag
     3661 agagcgtgtt cgaatacgat ctgattaagg acaagcggtt caccgaagat aagttctttt
     3721 tccattgtcc aatcaccatt aacttcaagt caagcggcgc taacaagttc aacgacgaga
     3781 tcaatctgct gctgaaggaa aaagcaaacg atgtgcacat cctgagcatt gaccgaggag
     3841 agcggcatct ggcctactat accctggtgg atggcaaagg gaatatcatt aagcaggata
     3901 cattcaacat cattggcaat gaccggatga aaaccaacta ccacgataaa ctggctgcaa
     3961 tcgagaagga tagagactca gctaggaagg actggaagaa aatcaacaac attaaggaga
     4021 tgaaggaagg ctatctgagc caggtggtcc atgagattgc aaagctggtc atcgaataca
     4081 atgccattgt ggtgttcgag gatctgaact tcggctttaa gagggggcgc tttaaggtgg
     4141 aaaaacaggt ctatcagaag ctggagaaaa tgctgatcga aaagctgaat tacctggtgt
     4201 ttaaagataa cgagttcgac aagaccggag gcgtcctgag agcctaccag ctgacagctc
     4261 cctttgaaac tttcaagaaa atgggaaaac agacaggcat catctactat gtgccagccg
     4321 gattcacttc caagatctgc cccgtgaccg gctttgtcaa ccagctgtac cctaaatatg
     4381 agtcagtgag caagtcccag gaatttttca gcaagttcga taagatctgt tataatctgg
     4441 acaaggggta cttcgagttt tccttcgatt acaagaactt cggcgacaag gccgctaagg
     4501 ggaaatggac cattgcctcc ttcggatctc gcctgatcaa ctttcgaaat tccgataaaa
     4561 accacaattg ggacactagg gaggtgtacc caaccaagga gctggaaaag ctgctgaaag
     4621 actactctat cgagtatgga catggcgaat gcatcaaggc agccatctgt ggcgagagtg
     4681 ataagaaatt tttcgccaag ctgacctcag tgctgaatac aatcctgcag atgcggaact
     4741 caaagaccgg gacagaactg gactatctga ttagccccgt ggctgatgtc aacggaaact
     4801 tcttcgacag cagacaggca cccaaaaata tgcctcagga tgcagacgcc aacggggcct
     4861 accacatcgg gctgaaggga ctgatgctgc tgggccggat caagaacaat caggagggga
     4921 agaagctgaa cctggtcatt aagaacgagg aatacttcga gtttgtccag aatagaaata
     4981 acaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag ggatcctacc
     5041 catacgatgt tccagattac gcttatccct acgacgtgcc tgattatgca tacccatatg
     5101 atgtccccga ctatgcccca aagaagaagc ggaaggtcga tggttctgaa aacctgtact
     5161 tccaatccat gaaaatcgaa gaaggtaaac tggtaatctg gattaacggc gataaaggct
     5221 ataacggtct cgctgaagtc ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg
     5281 ttgagcatcc ggataaactg gaagagaaat tcccacaggt tgcggcaact ggcgatggcc
     5341 ctgacattat cttctgggca cacgaccgct ttggtggcta cgctcaatct ggcctgttgg
     5401 ctgaaatcac cccggacaaa gcgttccagg acaagctgta tccgtttacc tgggatgccg
     5461 tacgttacaa cggcaagctg attgcttacc cgatcgctgt tgaagcgtta tcgctgattt
     5521 ataacaaaga tctgctgccg aacccgccaa aaacctggga agagatcccg gcgctggata
     5581 aagaactgaa agcgaaaggt aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca
     5641 cctggccgct gattgctgct gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg
     5701 acattaaaga cgtgggcgtg gataacgctg gcgcgaaagc gggtctgacc ttcctggttg
     5761 acctgattaa aaacaaacac atgaatgcag acaccgatta ctccatcgca gaagctgcct
     5821 ttaataaagg cgaaacagcg atgaccatca acggcccgtg ggcatggtcc aacatcgaca
     5881 ccagcaaagt gaattatggt gtaacggtac tgccgacctt caagggtcaa ccatccaaac
     5941 cgttcgttgg cgtgctgagc gcaggtatta acgccgccag tccgaacaaa gagctggcaa
     6001 aagagttcct cgaaaactat ctgctgactg atgaaggtct ggaagcggtt aataaagaca
     6061 aaccgctggg tgccgtagcg ctgaagtctt acgaggaaga gttggcgaaa gatccacgta
     6121 ttgccgccac tatggaaaac gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt
     6181 ccgctttctg gtatgccgtg cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg
     6241 tcgatgaagc cctgaaagac gcgcagactc tcgagcacca ccaccaccac cactgagatc
     6301 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
     6361 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
     6421 ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6481 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     6541 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     6601 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     6661 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     6721 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     6781 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     6841 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     6901 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     6961 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7021 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7081 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7141 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     7201 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7261 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7321 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7381 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     7441 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     7501 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     7561 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     7621 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     7681 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     7741 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     7801 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     7861 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     7921 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     7981 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8041 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8101 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8161 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     8221 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8281 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8341 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8401 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8461 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8521 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8581 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8641 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     8701 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     8761 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     8821 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     8881 cgcaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     8941 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     9001 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     9061 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg
     9121 gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc
     9181 cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt
     9241 aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat
     9301 gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga
     9361 acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga
     9421 ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc
     9481 acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga
     9541 cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca
     9601 ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt
     9661 ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat
     9721 catgcgcacc cgtggggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt
     9781 ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag
     9841 cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag
     9901 cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac
     9961 gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat
    10021 cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgctcact
    10081 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    10141 ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg
    10201 gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc
    10261 tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg
    10321 agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg cgcagcccgg
    10381 actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag
    10441 tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc
    10501 agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat
//
