LOCUS       sequence-242294-       11206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in 
            Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11206)
  AUTHORS   Zhang Q, Schepis A, Huang H, Yang J, Ma W, Torra J, Zhang SQ, Yang 
            L, Wu H, Nonell S, Dong Z, Kornberg TB, Coughlin SR, Shu X
  TITLE     Designing a green fluorogenic protease reporter by flipping a beta 
            strand of GFP for imaging apoptosis in animals.
  JOURNAL   J Am Chem Soc. 2019 Mar 1. doi: 10.1021/jacs.8b13042.
  PUBMED    30821975
REFERENCE   2  (bases 1 to 11206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2019 Mar 1. doi: 10.1021/jacs.8b13042."
COMMENT     Sequence Label: pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry
FEATURES             Location/Qualifiers
     source          1..11206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    62..156
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        175..413
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     360..381
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             424..435
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     intron          486..618
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     601..620
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             1053..1064
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1056..1109
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1719..1772
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1779..2489
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1924..1942)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2192..2211
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     intron          2573..2638
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2768..2788
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3060..3194
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3097..3116)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3151..3170
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3221..3239)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(3691..3762,4164..4437,4512..5166,5228..5543,5747..5878,
                     5949..6563)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(7342..7896,7897..7927)
                     /label=P element 5' end
     rep_origin      complement(8162..8750)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8242..8261)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8921..9712,9713..9781))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9544..9563
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9782..9886)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9954..9972
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10010..10032)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10132..10151
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    10361..10430
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    join(10974..11004,11005..11206)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 aagcttggct gcatccaacg cgttgggagc tctccggatc caagcttgca tgcctgcagg
       61 tcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      121 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactctagcg agcgccggag
      181 tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca
      241 cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatctgc
      301 agtaaagtgc aagttaaagt gaatcaatta aaagtaacca gcaaccaagt aaatcaactg
      361 caactactga aatctgccaa gaagtaatta ttgaatacaa gaagagaact ctgaataggg
      421 aattgggaat tgcagtgact ctcttaaggt agccttgcag aagttggtcg tgaggcactg
      481 ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa ctgggcttgt
      541 cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact gacatccact
      601 ttgcctttct ctccacaggt gtccactccc agttcaatta cagctcgaat tccaaaatgg
      661 acctgcctga cgaccactac ctgtccaccc agaccatcct gtccaaggac ctgaactccg
      721 gactcagatc tggcagcggt ctcgagatgg aagttagcgc tctggaaaaa gaagtgtctg
      781 cactcgagaa agaagtaagt gcccttgaga aggaggtgtc cgcactcgag aaggaggtca
      841 gcgccctgga aaaggaaaag cgagaccata tggttttgct tgagtatgtt acagcggctg
      901 gcattaccga tgcatcaggt gatgaagttg atggtggcgg tggcaaggtg tccgccctga
      961 aggaaaaagt aagcgcactg aaagaaaagg tgagcgcgct gaaggagaaa gtgagcgccc
     1021 tgaaagagaa agtctctgcc cttaaggagg atatcgaggg cagaggaagt ctgctaacat
     1081 gcggtgacgt cgaggagaat cctggcccaa agcttgccac catgcgcaaa ggcgaagaac
     1141 tgtttaccgg cattgtgccg attctggtgg aactggatgg cgatgtgaac ggccataaat
     1201 tttttgtgcg cggcgaaggc gaaggcgatg cgaccattgg caaactgagc ctgaaattta
     1261 tttgcaccac cggcaaactg ccggtgccgt ggccgaccct ggtgaccacc ctgacctatg
     1321 gcgtgcagtg ctttagccgc tatccggatc atatgaaacg ccatgatttt tttaaaagcg
     1381 cgatgccgga aggctatgtg caggaacgca ccatttattt taaagatgat ggcacctata
     1441 aaacccgcgc ggaagtgaaa tttgaaggcg ataccctggt gaaccgcatt gaactgaaag
     1501 gcattgattt taaagaagat ggcaacattc tgggccataa actggaatat aactttaaca
     1561 gccataaagt gtatattacc gcggataaac agaacaacgg cattaaagcg aactttacca
     1621 ttcgccataa cgtggaagat ggcagcgtgc agctggcgga tcattatcag cagaacaccc
     1681 cgattggcga tggcccggtt cttcttcctg gcggccgcga gggcagagga agtctgctaa
     1741 catgcggtga cgtcgaggag aatcctggcc cagaattgat ggtgagcaag ggcgaggagg
     1801 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     1861 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     1921 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     1981 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     2041 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     2101 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     2161 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     2221 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     2281 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     2341 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     2401 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     2461 ccggcggcat ggacgagctg tacaagtaat ctagaggatc tttgtgaagg aaccttactt
     2521 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
     2581 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
     2641 tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
     2701 gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
     2761 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
     2821 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
     2881 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt tgatgtatag
     2941 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     3001 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     3061 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3121 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtnnctt
     3181 atcatgtctg gatccactag aaggcctaat tctagtatgt atgtaagtta ataaaaccca
     3241 tttttgcgga aagtagataa aaaaaacatt tttttttttt actgcactgg atatcattga
     3301 acttatctga tcagttttaa atttacttcg atccaagggt atttgatgta ccaggttctt
     3361 tcgattacct ctcactcaaa atgacattcc actcaaagtc agcgctgttt gcctccttct
     3421 ctgtccacag aaatatcgcc gtctctttcg ccgctgcgtc cgctatctct ttcgccaccg
     3481 tttgtagcgt tacgtagcgt caatgtccgc cttcagttgc attttgtcag cggtttcgtg
     3541 acgaagctcc aagcggttta cgccatcaat taaacacaaa gtgctgtgcc aaaactcctc
     3601 tcgcttctta tttttgtttg ttttttgagt gattggggtg gtgattggtt ttgggtgggt
     3661 aagcagggga aagtgtgaaa aatcccggca atgggccaag aggatcagga gctattaatt
     3721 cgcggaggca gcaaacaccc atctgccgag catctgaaca atgtgagtag tacatgtgca
     3781 tacatcttaa gttcacttga tctataggaa ctgcgattgc aacatcaaat tgtctgcggc
     3841 gtgagaactg cgacccacaa aaatcccaaa ccgcaattgc acaaacaaat agtgacacga
     3901 aacagattat tctggtagct gttctcgcta tataagacaa tttttgagat catatcatga
     3961 tcaagacatc taaaggcatt cattttcgac tatattcttt tttacaaaaa atataacaac
     4021 cagatatttt aagctgatcc tagatgcaca aaaaataaat aaaagtataa acctacttcg
     4081 taggatactt cggggtactt tttgttcggg gttagatgag cataacgctt gtagttgata
     4141 tttgagatcc cctatcattg cagggtgaca gcggagcggc ttcgcagagc tgcattaacc
     4201 agggcttcgg gcaggccaaa aactacggca cgctccggcc acccagtccg ccggaggact
     4261 ccggttcagg gagcggccaa ctagccgaga acctcaccta tgcctggcac aatatggaca
     4321 tctttggggc ggtcaatcag ccgggctccg gatggcggca gctggtcaac cggacacgcg
     4381 gactattctg caacgagcga cacataccgg cgcccaggaa acatttgctc aagaacggtg
     4441 agtttctatt cgcagtcggc tgatctgtgt gaaatcttaa taaagggtcc aattaccaat
     4501 ttgaaactca gtttgcggcg tggcctatcc gggcgaactt ttggccgtga tgggcagttc
     4561 cggtgccgga aagacgaccc tgctgaatgc ccttgccttt cgatcgccgc agggcatcca
     4621 agtatcgcca tccgggatgc gactgctcaa tggccaacct gtggacgcca aggagatgca
     4681 ggccaggtgc gcctatgtcc agcaggatga cctctttatc ggctccctaa cggccaggga
     4741 acacctgatt ttccaagcca tggtgcggat gccacgacat ctgacctatc ggcagcgagt
     4801 ggcccgcgtg gatcaggtga tccaggagct ttcgctcagc aaatgtcagc acacgatcat
     4861 cggtgtgccc ggcagggtga aaggtctgtc cggcggagaa aggaagcgtc tggcattcgc
     4921 ctccgaggct ctaaccgatc cgccgcttct gatctgcgat gagcccacct ccggactgga
     4981 ctcctttacc gcccacagcg tcgtccaggt gctgaagaag ctgtcgcaga agggcaagac
     5041 cgtcatcctg accattcatc agccgtcttc cgagctgttt gagctctttg acaagatcct
     5101 tctgatggcc gagggcaggg tagctttctt gggcactccc agcgaagccg tcgacttctt
     5161 ttcctagtga gttcgatgtg tttattaagg gtatctagta ttacataaca tctcaactcc
     5221 tatccagcgt gggtgcccag tgtcctacca actacaatcc ggcggacttt tacgtacagg
     5281 tgttggccgt tgtgcccgga cgggagatcg agtcccgtga tcggatcgcc aagatatgcg
     5341 acaattttgc cattagcaaa gtagcccggg atatggagca gttgttggcc accaaaaatc
     5401 tggagaagcc actggagcag ccggagaatg ggtacaccta caaggccacc tggttcatgc
     5461 agttccgggc ggtcctgtgg cgatcctggc tgtcggtgct caaggaacca ctcctcgtaa
     5521 aagtgcgact tattcagaca acggtgagtg gttccagtgg aaacaaatga tataacgctt
     5581 acaattcttg gaaacaaatt cgctagattt tagatagaat tgcctgattc cacacccttc
     5641 ttagtttttt tcaatgagat gtatagttta tagttttgca gaagataaat aaatttcatt
     5701 taactcgcga atattaatga gatgcgagta acattttaat ttgcagatgg ttgccatctt
     5761 gattggcctc atctttttgg gccaacaact cacgcaagtg ggtgtgatga atatcaacgg
     5821 agccatcttc ctcttcctga ccaacatgac ctttcaaaac gtctttgcca cgataaatgt
     5881 aagtcatgtt tagaatacat ttgcatttca ataatttact aactttctaa tgaatcgatt
     5941 cgatttaggt gttcacctca gagctgccag tttttatgag ggaggcccga agtcgacttt
     6001 atcgctgtga cacatacttt ctgggcaaaa cgattgccga attgccgctt tttctcacag
     6061 tgccactggt cttcacggcg attgcctatc cgatgatcgg actgcgggcc ggagtgctgc
     6121 acttcttcaa ctgcctggcg ctggtcactc tggtggccaa tgtgtcaacg tccttcggat
     6181 atctaatatc ctgcgccagc tcctcgacct cgatggcgct gtctgtgggt ccgccggtta
     6241 tcataccatt cctgctcttt ggcggcttct tcttgaactc gggctcggtg ccagtatacc
     6301 tcaaatggtt gtcgtacctc tcatggttcc gttacgccaa cgagggtctg ctgattaacc
     6361 aatgggcgga cgtggagccg ggcgaaatta gctgcacatc gtcgaacacc acgtgcccca
     6421 gttcgggcaa ggtcatcctg gagacgctta acttctccgc cgccgatctg ccgctggact
     6481 acgtgggtct ggccattctc atcgtgagct tccgggtgct cgcatatctg gctctaagac
     6541 ttcgggcccg acgcaaggag tagccgacat atatccgaaa taactgcttg tttttttttt
     6601 ttaccattat taccatcgtg tttactgttt attgccccct caaaaagcta atgtaattat
     6661 atttgtgcca ataaaaacaa gatatgacct atagaataca agtatttccc cttcgaacat
     6721 ccccacaagt agactttgga tttgtcttct aaccaaaaga cttacacacc tgcatacctt
     6781 acatcaaaaa ctcgtttatc gctacataaa acaccgggat atatttttta tatacatact
     6841 tttcaaatcg cgcgccctct tcataattca cctccaccac accacgtttc gtagttgctc
     6901 tttcgctgtc tcccacccgc tctccgcaac acattcacct tttgttcgac gaccttggag
     6961 cgactgtcgt tagttccgcg cgattcggtt cgctcaaatg gttccgagtg gttcatttcg
     7021 tctcaataga aattagtaat aaatatttgt atgtacaatt tatttgctcc aatatatttg
     7081 tatatatttc cctcacagct atatttattc taatttaata ttatgacttt ttaaggtaat
     7141 tttttgtgac ctgttcggag tgattagcgt tacaatttga actgaaagtg acatccagtg
     7201 tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc ataatagtgt tgtttatata
     7261 tatcaaaaat aacaactata ataataagaa tacatttaat ttagaaaatg cttggatttc
     7321 actggaacta gaattaattc ggctgctgct ctaaacgacg catttcgtac tccaaagtac
     7381 gaattttttc cctcaagctc ttattttcat taaacaatga acaggaccta acgcacagtc
     7441 acgttattgt ttacataaat gatttttttt actattcaaa cttactctgt ttgtgtactc
     7501 ccactggtat agccttcttt tatcttttct ggttcaggct ctatcacttt actaggtacg
     7561 gcatctgcgt tgagtcgcct ccttttaaat gtctgacctt ttgcaggtgc agccttccac
     7621 tgcgaatcat taaagtgggt atcacaaatt tgggagtttt caccaaggct gcacccaagg
     7681 ctctgctccc acaattttct cttaatagca cacttcggca cgtgaattaa ttttactcca
     7741 gtcacagctt tgcagcaaaa tttgcaatat ttcatttttt tttattccac gtaagggtta
     7801 atgttttcaa aaaaaaattc gtccgcacac aacctttcct ctcaacaagc aaacgtgcac
     7861 tgaatttaag tgtatacttc ggtaagcttc ggctatcgac gggaccacct tatgttattt
     7921 catcatgggc cagacccacg tagtccagcg gcagatcggc ggcggagaag ttaagcgtct
     7981 ccaggatgac cttgcccgaa ctggggcacg tggtgttcga cgatgtgcag ctaatttcgc
     8041 ccggctccac gtccgcccat tggttaatca gcagaccctc gttggcgtaa cggaaccatg
     8101 agaggtacga caaccatttg aggtatactg gcaccgagcc cgagttcaag aagaaggcgt
     8161 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8221 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8281 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8341 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8401 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8461 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8521 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8581 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     8641 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8701 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8761 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8821 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8881 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8941 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9001 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9061 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9121 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9181 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9241 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9301 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9361 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9421 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9481 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9541 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9601 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9661 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9721 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9781 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9841 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9901 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     9961 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
    10021 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
    10081 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
    10141 agcagattgt actgagagtg caccatatcg acatgcccgc cgtgaccgtc gagaacccgc
    10201 tgacgctgcc ccgcgtatcc gcacccgccg acgccgtcgc acgtcccgtg ctcaccgtga
    10261 ccaccgcgcc cagcggtttc gagggcgagg gcttcccggt gcgccgcgcg ttcgccggga
    10321 tcaactaccg ccacctcgac ccgttcatca tgatggacca gatgggtgag gtggagtacg
    10381 cgcccgggga gcccaagggc acgccctggc acccgcaccg cggcttcgag accgtgacct
    10441 acatcgtcga tatgcggtgt gaaataccgc accgaatcgc gcggaactaa cgacagtcgc
    10501 tccaaggtcg tcgaacaaaa ggtgaatgtg ttgcggagag cgggtgggag acagcgaaag
    10561 agcaactacg aaacgtggtg tggtggaggt gaattatgaa gagggcgcgc gatttgaaaa
    10621 gtatgtatat aaaaaatata tcccggtgtt ttatgtagcg ataaacgagt ttttgatgta
    10681 aggtatgcag gtgtgtaagt cttttggtta gaagacaaat ccaaagtcta cttgtgggga
    10741 tgttcgaagg ggaaatactt gtattctata ggtcatatct tgtttttatt ggcacaaata
    10801 taattacatt agctttttga gggggcaata aacagtaaac acgatggtaa taatggtaaa
    10861 aaaaaaaaac aagcagttat ttcggatata tgtcggctac tccttgcgtc gggcccgaag
    10921 tcttagagcc agatatgcga gcacccggaa gctcacgatg agaatggcca gaccatgatg
    10981 aaataacata aggtggtccc gtcggcaaga gacatccact taacgtatgc ttgcaataag
    11041 tgcgagtgaa aggaatagta ttctgagtgt cgtattgagt ctgagtgaga cagcgatatg
    11101 attgttgatt aacccttagc atgtccgtgg ggtttgaatt aactcataat attaattaga
    11161 cgaaattatt tttaaagttt tatttttaat aatttgcgag tacgca
//
