LOCUS       sequence-242318-       11788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  donorAB-Neon targeting vector for human AAVS1 locus; knock-in of Dox
            controlled H2BGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11788)
  AUTHORS   Tejero R, Huang Y, Katsyv I, Kluge M, Lin JY, Tome-Garcia J, Daviaud
            N, Wang Y, Zhang B, Tsankova NM, Friedel CC, Zou H, Friedel RH
  TITLE     Gene signatures of quiescent glioblastoma cells reveal mesenchymal 
            shift and interactions with niche microenvironment.
  JOURNAL   EBioMedicine. 2019 Apr 2. pii: S2352-3964(19)30209-9. doi: 
            10.1016/j.ebiom.2019.03.064.
  PUBMED    30952620
REFERENCE   2  (bases 1 to 11788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "EBioMedicine. 2019 Apr 2. pii: S2352-3964(19)30209-9. doi: 
            10.1016/j.ebiom.2019.03.064."
COMMENT     Sequence Label: pAAVS1-Neo-CAG-M2rtTA-H2BmNeon (donorAB-mNeon)
FEATURES             Location/Qualifiers
     source          1..11788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          100..1116
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1040..1062
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1124..1143
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(1234..1851,1852..1857,1858..1893,1894..1896,1897..1932,
                     1933..1935,1936..1971,1972..1980)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    1990..2124
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2014..2033)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    2475..2556
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2703..3413)
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      3149..3158
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3410..3419
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3432..3809)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     promoter        complement(3838..4152)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(3841..3865)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3912..3930
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3948..3966
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3983..4001
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4019..4037
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4055..4073
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4090..4108
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4126..4144
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    4200..5036
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(5070..5089)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5071..5089)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5096..5113)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5096..5112)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5105..5127)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             5250..5552
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5431..5450
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(join(6949..7740,7741..7809))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7572..7591
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7933..8521
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8422..8441
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8675..8692
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8809..8830
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8845..8850,8851..8868,8869..8875)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8883..8899
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8888..8910
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8907..8923
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8907..8923
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8944..8962
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    8997..9800
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    9807..9832
                     /label=SA
                     /note="splice acceptor site"
     CDS             9856..9909
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             9925..10728
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     enhancer        11354..11657
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
       61 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
      121 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
      181 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
      241 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
      301 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
      361 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
      421 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
      481 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
      541 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
      601 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg
      661 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
      721 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
      781 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
      841 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
      901 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
      961 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     1021 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
     1081 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     1141 gtgctgtctc atcattttgg caaagaattc ggcttgatcg aagctagtgg atcccccggg
     1201 ctgcaggaat tcaccatgtc tagactggac aagatgtcta gactggacaa gagcaaagtc
     1261 ataaacggcg ctctggaatt actcaatgga gtcggtatcg aaggcctgac gacaaggaaa
     1321 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg
     1381 gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc
     1441 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcatt ccgctgtgct
     1501 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag
     1561 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac
     1621 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag
     1681 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt
     1741 ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc
     1801 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccggcc
     1861 gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac
     1921 cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa
     1981 ctaagtaagg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     2041 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     2101 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     2161 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     2221 gattatgatc ctgcaagcct cgtcgtcctg gccggaccac gctatctgtg caaggtcccc
     2281 ggccccggac gcgcgctcca tgagcagagc gcccgccgcc gaggcgaaga ctcgggcggc
     2341 gccctgcccg tcccaccagg tcaacaggcg gtaaccggcc tcttcatcgg gaatgcgcgc
     2401 gaccttcagc atcgccggca tgtccccctg gcggacggga agtatccagc tcgaccaagc
     2461 ttagtactgt cgaggcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     2521 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     2581 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     2641 tgtggtatgg ctgattatga tcctctagag atatcgtcga caagcttatc gatgcggccg
     2701 ctttacttgt acagctcgtc catgcccatc acatcggtaa aggccttttg ccactccttg
     2761 aagttgagct cggtcttgga gtgcttgagc tccgtcttac ggaacacgta catcggctgg
     2821 ttcttcagat agttagccgc cattggcttg gcaaaggtgt aggtggtccg cgcagtgctc
     2881 cggtagcgct tgccatttcc agtggtgtaa ctccacttaa aggtactgat gatggttttg
     2941 tcgttggggt aagtcttctt cgacctgcac cagtccgcag cggtcagcga gttggtcatc
     3001 acaggaccgt cagcagggaa accagtcccc ttcacctggg cctctccttt gatgtggctt
     3061 ccctcgtagg tgtagcggta gttaacagta agggaggcac catcttcaaa ctgcattgtg
     3121 cgatggactt ggtatccgga gccatctacc atggcggcct ggaaaggcga catcccgtca
     3181 gggtagggca ggtactgatg gaagccatac ccgatatgag ggaccagaat ccagggggag
     3241 aactggaggt cacccttggt ggacttcagg tttaactcct cataaccatc atttggattg
     3301 ccggtgccct gacccaccat gtcaaagtcc acaccgttga tggagccaaa gatgtgtaac
     3361 tcatgtgtcg ctgggagaga ggccatgtta tcctcctcgc ccttgctcac catggtggcg
     3421 accggtggat ccttagcgct ggtgtacttg gtgatggcct tagtaccctc ggacacggcg
     3481 tgcttggcca actccccagg cagcagcagg cgcacggccg tctggatctc cctggaggtg
     3541 atggtcgagc gcttgttgta atgcgccagg cgggaagcct cacctgcgat gcgctcgaaa
     3601 atgtcgttca caaacgaatt catgatgccc atggccttgg acgaaatgcc ggtgtcaggg
     3661 tggacctgct tcagaacctt gtacacatag atggaatagc tctccttgcg gctgcgcttg
     3721 cgcttcttgc cgcctttctt ctgcgcctta gtcaccgcct tcttggagcc ctttttcggg
     3781 gcgggagcag acttcgctgg ctctggcatg gtggcggtac cgagctcgaa ttctccaggc
     3841 gatctgacgg ttcactaaac gagctctgct tatataggcc tcccaccgta cacgcctacc
     3901 tcgacatacg ttctctatca ctgataggga gtaaactcga catacgttct ctatcactga
     3961 tagggataaa ctcgacatac gttctctatc actgataggg agtaaactcg acatacgttc
     4021 tctatcactg atagggagta aactcgacat acgttctcta tcactgatag ggagtaaact
     4081 cgacatcgtt ctctatcact gatagggagt aaactcgaca tacgttctct atcactgata
     4141 gggagtaaac tcgacccata gagcccaccg catccccagc atgcctgcta ttaagcttta
     4201 ctagggacag gattggtgac agaaaagccc catccttagg cctcctcctt cctagtctcc
     4261 tgatattggg tctaaccccc acctcctgtt aggcagattc cttatctggt gacacacccc
     4321 catttcctgg agccatctct ctccttgcca gaacctctaa ggtttgctta cgatggagcc
     4381 agagaggatc ctgggaggga gagcttggca gggggtggga gggaaggggg ggatgcgtga
     4441 cctgcccggt tctcagtggc caccctgcgc taccctctcc cagaacctga gctgctctga
     4501 cgcggctgtc tggtgcgttt cactgatcct ggtgctgcag cttccttaca cttcccaaga
     4561 ggagaagcag tttggaaaaa caaaatcaga ataagttggt cctgagttct aactttggct
     4621 cttcaccttt ctagtcccca atttatattg ttcctccgtg cgtcagtttt acctgtgaga
     4681 taaggccagt agccagcccc gtcctggcag ggctgtggtg aggagggggg tgtccgtgtg
     4741 gaaaactccc tttgtgagaa tggtgcgtcc taggtgttca ccaggtcgtg gccgcctcta
     4801 ctccctttct ctttctccat ccttctttcc ttaaagagtc cccagtgcta tctgggacat
     4861 attcctccgc ccagagcagg gtcccgcttc cctaaggccc tgctctgggc ttctgggttt
     4921 gagtccttgg caagcccagg agaggcgctc aggcttccct gtcccccttc ctcgtccacc
     4981 atctcatgcc cctggctctc ctgccccttc cctacagggg ttcctggctc tgctctaagg
     5041 gcgaattcgc ggccgctaaa ttcaattcgc cctatagtga gtcgtattac aattcactgg
     5101 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     5161 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     5221 cccaacagtt gcgcagccta tacgtacggc agtttaaggt ttacacctat aaaagagaga
     5281 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccg gggcgacgga
     5341 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
     5401 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
     5461 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
     5521 acgccattaa cctgatgttc tggggaatat aaatgtcagg catgagatta tcaaaaagga
     5581 tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc tgaccccgga
     5641 tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg
     5701 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
     5761 aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
     5821 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagctct gatcaagaga
     5881 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     5941 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     6001 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     6061 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     6121 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     6181 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     6241 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     6301 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     6361 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     6421 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatgcatgg cgatgcctgc
     6481 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     6541 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     6601 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     6661 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttattaacgc ttacaatttc
     6721 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat caggtggcac
     6781 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6841 gtatccgctc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6901 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6961 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7021 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7081 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7141 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7201 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7261 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7321 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7381 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7441 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7501 atactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7561 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7621 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7681 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7741 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7801 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7861 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7921 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7981 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8041 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     8101 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8161 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8221 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8281 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8341 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8401 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8461 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8521 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8581 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8641 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8701 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8761 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8821 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     8881 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     8941 cagaattaac cctcactaaa gggactagtc ctgcaggttt aaacgaattc gccctttgct
     9001 ttctctgacc agcattctct cccctgggcc tgtgccgctt tctgtctgca gcttgtggcc
     9061 tgggtcacct ctacggctgg cccagatcct tccctgccgc ctccttcagg ttccgtcttc
     9121 ctccactccc tcttcccctt gctctctgct gtgttgctgc ccaaggatgc tctttccgga
     9181 gcacttcctt ctcggcgctg caccacgtga tgtcctctga gcggatcctc cccgtgtctg
     9241 ggtcctctcc gggcatctct cctccctcac ccaaccccat gccgtcttca ctcgctgggt
     9301 tcccttttcc ttctccttct ggggcctgtg ccatctctcg tttcttagga tggccttctc
     9361 cgacggatgt ctcccttgcg tcccgcctcc ccttcttgta ggcctgcatc atcaccgttt
     9421 ttctggacaa ccccaaagta ccccgtctcc ctggctttag ccacctctcc atcctcttgc
     9481 tttctttgcc tggacacccc gttctcctgt ggattcgggt cacctctcac tcctttcatt
     9541 tgggcagctc ccctaccccc cttacctctc tagtctgtgc tagctcttcc agccccctgt
     9601 catggcatct tccaggggtc cgagagctca gctagtcttc ttcctccaac ccgggcccct
     9661 atgtccactt caggacagca tgtttgctgc ctccagggat cctgtgtccc cgagctggga
     9721 ccaccttata ttcccagggc cggttaatgt ggctctggtt ctgggtactt ttatctgtcc
     9781 cctccacccc acagtggggc aagcttctga cctcttctct tcctcccaca gggcctcgag
     9841 agatctggca gcggagaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
     9901 cccggcccta ggctcgagac gcgtatggga tcggccattg aacaagatgg attgcacgca
     9961 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
    10021 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
    10081 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
    10141 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
    10201 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
    10261 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
    10321 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
    10381 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
    10441 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
    10501 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
    10561 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
    10621 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
    10681 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagg ggatccgctg
    10741 taagtctgca gaaattgatg atctattaaa caataaagat gtccactaaa atggaagttt
    10801 ttcctgtcat actttgttaa gaagggtgag aaaagatgtc cactaaaatg gaagtttttc
    10861 ctgtcatact ttgttaagaa gggtgagaac agagtaccta cattttgaat ggaaggattg
    10921 gagctacggg ggtgggggtg gggtgggatt agataaatgc ctgctcttta ctgaaggctc
    10981 tttactattg ctttatgata atgtttcata gttggatatc ataatttaaa caagcaaaac
    11041 caaattaagg gccagctcat tcctcccact catgatctat agatctatag atctctcgtg
    11101 ggatcattgt ttttctcttg attcccactt tgtggttcta agtactgtgg tttccaaatg
    11161 tgtcagtttc atagcctgaa gaacgagatc agcagcctct gttccacata cacttcattc
    11221 tcagtattgt tttgccaagt tctaattcca tcagaagctg ggagctccac cgcggtggcg
    11281 gccgctctag aactagttat taatagtaat caattacggg gtcattagtt catagcccat
    11341 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
    11401 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
    11461 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
    11521 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
    11581 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
    11641 tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
    11701 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
    11761 gggcgggggg gggggggggg cgcgcgcc
//
