LOCUS       sequence-242348-        9598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Lin Y, McDonough SI, Lipscombe D
  TITLE     Alternative splicing in the voltage-sensing region of N-Type CaV2.2 
            channels modulates channel kinetics.
  JOURNAL   J Neurophysiol. 2004 Nov . 92(5):2820-30.
  PUBMED    15201306
REFERENCE   2  (bases 1 to 9598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurophysiol. 2004 Nov . 92(5):2820-30."
COMMENT     Sequence Label: CaV-alpha-2-delta-1
FEATURES             Location/Qualifiers
     source          1..9598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     752..768
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     752..768
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4786..4803)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4792..5016
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5062..5490
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5149..5168)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5359..5380
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5499..5519)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5504..5833
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5684..5819
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5746..5765
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5882..6907
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5882..6907
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    7037..7158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7074..7093)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7128..7147
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7207..7223)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7207..7223)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7220..7242)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7231..7247
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7255..7261,7262..7279,7280..7285))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7300..7321
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7438..7455)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7609..8197)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7689..7708)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8368..9159,9160..9228))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8991..9010
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9229..9333)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9408..9427)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
      721 ttctgcagat atccagcaca gtggcggccg ctctagaact agtggatccc ccgggctgca
      781 ggaattcggc acgagcgggg agccagagcg cgcgggcgcc ggcaggctgg ccgagctccg
      841 tttccacagt cgcccttgat ggcggcgcag gcgaggcagc cgtggcacgg agcggcagcc
      901 acgcgctaga gagtccgccc ggccccgccc ggccggcccg cccgtgccct gccctgcccg
      961 cctctctcca cgcgccctcc ggcagacgtc ggcgccccgg ctcagtccct cgccgcgtgc
     1021 agctccgccg cctccgggcc ggcagggagg gcactgatct tcgatcgcga agatggctgc
     1081 tggctgcctg ctggccttga ctctgacact tttccaatct tggctgatcg gcccctcgag
     1141 cgaggagccc ttcccttcgc ccgtcactat caagtcatgg gtggataaaa tgcaggaaga
     1201 ccttgtcaca ctggcaaaga cagcaagtgg agtgactcag cttgctgaca tttatgaaaa
     1261 ataccaagat ttgtatactg tggagccaaa taatgcacgc caactggttg aaattgcagc
     1321 tagagacatt gagaaacttc tgagcaacag atctaaagcc ctggtgcgcc tggctatgga
     1381 agcagagaaa gtccaagccg cccaccaatg gagggaagat tttgcaagca acgaagttgt
     1441 ctactacaac gctaaagacg atcttgatcc tgaaagaaat gagagtgagt caggcagcca
     1501 acggattaaa cctgttttca tcgaagacgc taattttgga cgccagatat cctatcaaca
     1561 tgcagcggtc catattccca cggacatcta tgagggctca accatagtgt taaacgaact
     1621 caactggaca agtgccttag atgaagtttt caaaagaaat cgagatgaag accctacatt
     1681 gctgtggcaa gtgtttggca gcgccaccgg cctggcccga tattatccag cttctccgtg
     1741 ggtggataac agcagaactc caaacaagat cgatctctac gatgtacgca gaagaccatg
     1801 gtacatccag ggagctgcat cccccaaaga catgctcatt ctggtggacg tgagtggaag
     1861 tgtgagcgga ttgactctga aactcatccg gacatctgtc tctgagatgt tagaaaccct
     1921 ttccgatgat gatttcgtga atgtagcttc atttaacagc aatgctcagg atgtgagttg
     1981 tttccagcac ctggttcaag cgaatgtaag gaacaagaag gtgctgaagg atgcggtgaa
     2041 taacatcaca gcaaagggga taacagatta caagaaaggc tttagcttcg cgtttgaaca
     2101 gctacttaat tataatgttt ccagagccaa ttgtaataag atcatcatgt tattcacgga
     2161 tggaggagaa gagagagccc aggagatatt tgccaagtac aataaagaca aaaaagtccg
     2221 cgtgtttaca ttttctgttg gtcaacataa ttatgacaga ggacctattc agtggatggc
     2281 ttgtgaaaac aaaggttatt attatgagat tccctctatt ggtgcaataa gaatcaatac
     2341 tcaggaatat ctagatgttc tgggaagacc aatggtttta gccggggaca aagcaaagca
     2401 agtccagtgg acaaatgtgt atttggatgc tctggagctg ggacttgtca ttactggaac
     2461 tctaccggtc ttcaacgtca ctggccagtc tgaaaataag actaacttga agaaccagtt
     2521 gattcttggt gtgatgggcg ttgatgtgtc tctggaagat attaagagat tgacaccacg
     2581 ttttacactg tgccccaacg gttactattt tgcaatcgat cctaatggtt atgtcttact
     2641 gcatccaaat cttcagccaa agaaccccaa atctcaggag ccggtcacac tggattttct
     2701 cgatgctgag ttagagaatg atattaaagt ggagattcga aataaaatga tagacggaga
     2761 aagtggagaa aaaacattca gaactctggt caaatctcaa gatgagagat atattgacaa
     2821 aggaaatcga acatacacat ggacacctgt caatggcaca gattacagtt tggccttggt
     2881 attgccaacc tacagttttt actatataaa agccaaaata gaagagacaa taactcaggc
     2941 cagatcaaaa aaggggaaaa tgaaggattc agaaaccctg aagccagaca attttgaaga
     3001 atctggctac actttcatag caccaaggga atactgcaat gacctgaaac cttcagataa
     3061 taacactgaa tttcttttaa atttcaatga atttattgat aggaaaactc caaacaaccc
     3121 ttcctgtaat acagatttga ttaatagaat cttgctggat gcgggtttta caaatgaact
     3181 tgtccaaaat tattggagta aacagaaaaa tatcaaagga gtgaaggcac gctttgtggt
     3241 gactgatggc ggaattacaa gagtttaccc caaagaggct ggagaaaact ggcaagagaa
     3301 cccagagacg tatgaggaca gcttctacaa acggagccta gataacgata actacgtttt
     3361 cactgcgccc tacttcaaca aaagtggacc tggtgcctat gaatctggaa ttatggtaag
     3421 caaagctgta gaactgtaca tccaaggaaa acttcttaag cctgcagttg tgggaattaa
     3481 aattgacgta aactcctgga tagaaaattt taccaaaact tcaatcaggg atccgtgtgc
     3541 tggtccagtt tgtgactgca aaagaaacag tgatgtaatg gactgtgtca ttctagatga
     3601 tggtggattt cttctgatgg ccaatcatga tgattacact aatcagattg gacgtttttt
     3661 tggagagatt gacccgagca tgatgagaca cctggttaat atatcacttt atgcattcaa
     3721 taaatcatat gactatcagt ctgtgtgtga tccaggggca gcaccaaagc aaggagcagg
     3781 acatcgctca gcctatgtgc catcaattac agacatactc cagattggct ggtgggccac
     3841 tgctgccgcc tggtctattc tccagcagct actcttgagt ttgacatttc cacggctcct
     3901 tgaggcagtt gaaatggaag aggatgactt cacagcttcc ctgtctaagc agagctgcat
     3961 cacagaacaa actcagtact tcttcaagaa cgatactaaa tcattcagtg gtttactgga
     4021 ctgtggaaac tgttccagga tctttcatgt tgagaagctt atgaacacca acctagtgtt
     4081 cataatggtg gagagcaagg gaacatgtcc gtgtgacacg cggctgctca tgcaagcaga
     4141 acagacttct gatggtccag atccttgcga catggttaag cagcccagat accgaaaagg
     4201 acctgatgtc tgctttgata acaatgtgct ggaggattat accgactgtg gtggtgtttc
     4261 tggattaaac ccttccttat ggtctatctt tggactccaa tttatactcc tttggctggt
     4321 atctggcagc agacactatc tatggtgacc ctctaaaccc atgatctgca acttaattcc
     4381 atgccctgcc aaaacatgag ccctcagtta cattaaaata gagcccgttg aagaagctcc
     4441 acagaacctt agctgggcct ctaccctgat gtaactgtga ggcttagatt cctgaccatc
     4501 gctggctgca tgtcagggtg ttggaaactg aaactcgtgt gaacatgctg catcatctgt
     4561 gtccaacatc aaagcaaagc tctactcatt ctctgtggga ggtttgggag ttcgctgttg
     4621 cactgttgga gattacgtgg aaaaggctcc cccacgcagg ccttatgaaa agaagcagtc
     4681 ttgactttga cctagcattt caacctgctg cagttttacc aagaagaaga aggaaaaaaa
     4741 aaaaaaaaaa actcgagggg gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     4801 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     4861 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     4921 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     4981 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     5041 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     5101 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     5161 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     5221 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     5281 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     5341 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     5401 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     5461 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     5521 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5581 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5641 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     5701 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     5761 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5821 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
     5881 gatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
     5941 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
     6001 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
     6061 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
     6121 tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
     6181 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga
     6241 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
     6301 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
     6361 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
     6421 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
     6481 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
     6541 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
     6601 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
     6661 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
     6721 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
     6781 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
     6841 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
     6901 ggaatagcac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     6961 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     7021 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7081 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7141 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     7201 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7261 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7321 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7381 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7441 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7501 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7561 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7621 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7681 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7741 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7801 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7861 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7921 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7981 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8041 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8101 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8161 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8221 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8281 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8341 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8401 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8461 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8521 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8581 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8641 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8701 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8761 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8821 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8881 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8941 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9001 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9061 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9121 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9181 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9241 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9301 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9361 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     9421 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     9481 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     9541 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgtt
//
