LOCUS       sequence-242353-        3039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing inactive NpuDnaB-Delta-283 mini-intein inserted 
            into CcdA antitoxin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Beyer HM, Iwai H
  TITLE     Off-pathway-sensitive protein splicing screening based on a 
            toxin/antitoxin system.
  JOURNAL   Chembiochem. 2019 Apr 8. doi: 10.1002/cbic.201900139.
  PUBMED    30963690
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2019 Apr 8. doi: 10.1002/cbic.201900139."
COMMENT     Sequence Label: pHBSTC159
FEATURES             Location/Qualifiers
     source          1..3039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      93..681
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     582..601
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     835..852
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     868..887
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1910..1928)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1996..2100
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2101..2169,2170..2961)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2319..2338)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
       61 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
      121 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
      181 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
      241 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      301 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      361 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      421 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      481 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
      541 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
      601 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
      661 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
      721 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
      781 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
      841 tcagtgagcg aggaagcgga aactaggcgg catcagagca gattgtactg agagtgcacc
      901 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggccgctta
      961 attaacccgg gccctctaga attctgcagc atgcttgccg cagccgccgt aggcggtcac
     1021 gactttgcga agcaaagtct agtgagtata ctcaagcatt gagtggcccg ccggaggcac
     1081 cgccttgcgc tgcccccgtc gagccggttg gtcaccaaaa gggaggggca ggcatgaagc
     1141 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tacgatgtca
     1201 atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
     1261 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
     1321 gaggtttagc aggcgatagt ctggtgacat tagtagatag cggactgcaa gtgccaatta
     1381 aggaattagt aggtaaatct ggttttgcag tttgggcatt gaacgaagct acaatgcagc
     1441 tagaaaaggc aattgttagc aatgcttttt cgactggtat aaagcctctg tttactttga
     1501 caactcgatt ggggcggaaa atccgtgcca caggtaatca caagttttta acaattaatg
     1561 gctggaaaag acttgatgaa ttaactccta aagaacatct cgctttacca agaaatctcc
     1621 ccagttctgg tactcttgcc aaaagtgata tttattggga tgaaatagtt tcaattgaat
     1681 atagtggtga ggaagaagtg tttgacctca ccgttcctgg tttgcataat tttgttgcaa
     1741 ataatattat tgttcacaat tccttcgccg actgaatcgt ttaaacgaat tcactagtgg
     1801 atccgagctc ggtaccaagc ttgatgcata gcttgagtat tctaacgcgc tgcattaatg
     1861 aatcggccaa cgcgcgggga gaggcggttt gcgtattgag cacgaaaggg cctcgtgata
     1921 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     1981 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2041 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2101 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2161 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     2221 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     2281 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     2341 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     2401 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     2461 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     2521 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     2581 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     2641 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     2701 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     2761 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     2821 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     2881 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     2941 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     3001 ttaaaacttc atttttaatt taaaaggatc taggtgaag
//
