LOCUS       sequence-242354-        3039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing NpuDnaB-Delta-283 mini-intein inserted into CcdA 
            antitoxin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Beyer HM, Iwai H
  TITLE     Off-pathway-sensitive protein splicing screening based on a 
            toxin/antitoxin system.
  JOURNAL   Chembiochem. 2019 Apr 8. doi: 10.1002/cbic.201900139.
  PUBMED    30963690
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2019 Apr 8. doi: 10.1002/cbic.201900139."
COMMENT     Sequence Label: pBHSTC97
FEATURES             Location/Qualifiers
     source          1..3039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(135..153)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        221..325
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(326..394,395..1186)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(544..563)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1357..1945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1846..1865
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2099..2116
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2132..2151
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atcgtttaaa cgaattcact agtggatccg agctcggtac caagcttgat gcatagcttg
       61 agtattctaa cgcgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
      121 ttgagcacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
      181 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      241 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      301 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      361 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      421 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
      481 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
      541 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
      601 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
      661 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
      721 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
      781 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
      841 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
      901 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
      961 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1021 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1081 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1141 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     1201 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1261 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1321 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1381 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1441 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1501 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1561 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1621 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1681 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1741 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1801 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1861 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1921 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     1981 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2041 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2101 cgagtcagtg agcgaggaag cggaaactag gcggcatcag agcagattgt actgagagtg
     2161 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggccg
     2221 cttaattaac ccgggccctc tagaattctg cagcatgctt gccgcagccg ccgtaggcgg
     2281 tcacgacttt gcgaagcaaa gtctagtgag tatactcaag cattgagtgg cccgccggag
     2341 gcaccgcctt gcgctgcccc cgtcgagccg gttggtcacc aaaagggagg ggcaggcatg
     2401 aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatacgat
     2461 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc
     2521 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg
     2581 aacggatgtt tagcaggcga tagtctggtg acattagtag atagcggact gcaagtgcca
     2641 attaaggaat tagtaggtaa atctggtttt gcagtttggg cattgaacga agctacaatg
     2701 cagctagaaa aggcaattgt tagcaatgct ttttcgactg gtataaagcc tctgtttact
     2761 ttgacaactc gattggggcg gaaaatccgt gccacaggta atcacaagtt tttaacaatt
     2821 aatggctgga aaagacttga tgaattaact cctaaagaac atctcgcttt accaagaaat
     2881 ctccccagtt ctggtactct tgccaaaagt gatatttatt gggatgaaat agtttcaatt
     2941 gaatatagtg gtgaggaaga agtgtttgac ctcaccgttc ctggtttgca taattttgtt
     3001 gcaaataata ttattgttca caattccttc gccgactga
//
