LOCUS       sequence-242356-        4883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabinose-inducible vector encoding the C-terminal gp41-1 split 
            fragment fused to GB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Beyer HM, Iwai H
  TITLE     Off-pathway-sensitive protein splicing screening based on a 
            toxin/antitoxin system.
  JOURNAL   Chembiochem. 2019 Apr 8. doi: 10.1002/cbic.201900139.
  PUBMED    30963690
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2019 Apr 8. doi: 10.1002/cbic.201900139."
COMMENT     Sequence Label: pBHBAD42
FEATURES             Location/Qualifiers
     source          1..4883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             73..95
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(427..444)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      597..683
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      775..802
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        821..912
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(913..981,982..1773)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1131..1150)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1815..2270
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1902..1921)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2112..2133
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      2381..2969
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2870..2889
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3123..3140
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3155..3295
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3221..3240)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3381..3403
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3541..3560
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(3740..4618)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        join(4645..4883,1..46)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4852..4871
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccc gtttttttgg
       61 gctagaaata attttgttta actttaagaa ggagatatac atatgatgct gaaaaagatt
      121 ctgaaaattg aagagctgga tgaacgcgaa ctgatcgata ttgaagtgag cggtaaccat
      181 ctgttttatg cgaacgacat tctgacccac aacagcggta cccagtacaa acttatcctg
      241 aacggtaaaa ccctgaaagg tgaaaccacc accgaagctg ttgacgctgc taccgcggaa
      301 aaagttttca aacagtacgc taacgacaac ggtgttgacg gtgaatggac ctacgacgac
      361 gctaccaaaa cctacaccgt aacggaataa gcttggctgt tttggcggat gagagaagat
      421 tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc
      481 tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg
      541 tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa
      601 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      661 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
      721 ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg
      781 ccatcctgac ggatggcctt tttgcgtttc tacaaactct tttgtttatt tttctaaata
      841 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      901 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
      961 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     1021 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     1081 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     1141 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     1201 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     1261 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     1321 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     1381 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     1441 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     1501 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     1561 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     1621 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     1681 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     1741 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     1801 ctttagattg atttacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     1861 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     1921 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     1981 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
     2041 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     2101 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta
     2161 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     2221 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt aaaaggatct
     2281 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2341 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2401 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2461 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2521 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2581 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2641 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2701 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2761 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2821 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2881 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2941 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3001 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3061 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3121 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     3181 atctgtgcgg tatttcacac cggatatggt gcactctcag tacaatctgc tctgatgccg
     3241 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
     3301 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     3361 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     3421 gaaacgcgcg aggcagaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
     3481 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc
     3541 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac
     3601 gatgcgtccg gcgtagagga tctgctcatg tttgacagct tatcatcgat gcataatgtg
     3661 cctgtcaaat ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa
     3721 ttgtctgatt cgttaccaat tatgacaact tgacggctac atcattcact ttttcttcac
     3781 aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat
     3841 agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc
     3901 tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc
     3961 ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga
     4021 cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc
     4081 gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta
     4141 acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg
     4201 cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa
     4261 agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg
     4321 gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat
     4381 gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct
     4441 gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca
     4501 gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca
     4561 ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac
     4621 ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc
     4681 gtcactgcgt cttttactgg ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa
     4741 gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata
     4801 atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag
     4861 catttttatc cataagatta gcg
//
