LOCUS       sequence-242359-        2976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing inactive gp41-1 intein inserted into CcdA 
            antitoxin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Beyer HM, Iwai H
  TITLE     Off-pathway-sensitive protein splicing screening based on a 
            toxin/antitoxin system.
  JOURNAL   Chembiochem. 2019 Apr 8. doi: 10.1002/cbic.201900139.
  PUBMED    30963690
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2019 Apr 8. doi: 10.1002/cbic.201900139."
COMMENT     Sequence Label: pHBSTC147
FEATURES             Location/Qualifiers
     source          1..2976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      100..688
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     589..608
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     842..859
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     875..894
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1854..1872)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1940..2044
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2045..2113,2114..2905)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2263..2282)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
       61 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
      121 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
      181 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
      241 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
      301 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
      361 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
      421 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
      481 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
      541 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
      601 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
      661 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
      721 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
      781 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
      841 cagcgagtca gtgagcgagg aagcggaaac taggcggcat cagagcagat tgtactgaga
      901 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
      961 ccgcttaatt aacccgggcc ctctagaatt ctgcagcatg cttgccgcag ccgccgtagg
     1021 cggtcacgac tttgcgaagc aaagtctagt gagtatactc aagcattgag tggcccgccg
     1081 gaggcaccgc cttgcgctgc ccccgtcgag ccggttggtc accaaaaggg aggggcaggc
     1141 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatac
     1201 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
     1261 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
     1321 atgaacggag gcctggatct gaaaacgcag gtgcagaccc cacagggcat gaaagagata
     1381 tccaacattc aggtgggcga tcttgttctg agcaacaccg gctataacga ggtgctgaac
     1441 gtgtttccga aaagcaaaaa gaaaagctat aaaattaccc tggaagatgg caaagaaatt
     1501 atttgtagcg aagaacatct gttcccgacc cagaccggcg aaatgaacat cagcggcggt
     1561 ctgaaagagg gcatgtgcct gtatgtgaaa gaaatgatgc tgaaaaagat tctgaaaatt
     1621 gaagagctgg atgaacgcga actgatcgat attgaagtga gcggtaacca tctgttttat
     1681 gcgaacgaca ttctgaccca caatagcttc gccgactgaa tcgtttaaac gaattcacta
     1741 gtggatccga gctcggtacc aagcttgatg catagcttga gtattctaac gcgctgcatt
     1801 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgagcacgaa agggcctcgt
     1861 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     1921 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     1981 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2041 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2101 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2161 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2221 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     2281 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     2341 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     2401 attatgtagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     2461 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     2521 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     2581 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     2641 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     2701 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     2761 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     2821 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     2881 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     2941 tgatttaaaa cttcattttt aatttaaaag gatcta
//
