LOCUS       sequence-242360-        2976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing gp41-1 intein inserted into CcdA antitoxin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Beyer HM, Iwai H
  TITLE     Off-pathway-sensitive protein splicing screening based on a 
            toxin/antitoxin system.
  JOURNAL   Chembiochem. 2019 Apr 8. doi: 10.1002/cbic.201900139.
  PUBMED    30963690
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2019 Apr 8. doi: 10.1002/cbic.201900139."
COMMENT     Sequence Label: pHYSTC299
FEATURES             Location/Qualifiers
     source          1..2976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(839..857)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        925..1029
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1030..1098,1099..1890)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1248..1267)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2061..2649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2550..2569
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2803..2820
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2836..2855
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtaggcggtc acgactttgc gaagcaaagt ctagtgagta tactcaagca ttgagtggcc
       61 cgccggaggc accgccttgc gctgcccccg tcgagccggt tggtcaccaa aagggagggg
      121 caggcatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg
      181 catacgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc
      241 tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta
      301 ttgaaatgaa cggatgcctg gatctgaaaa cgcaggtgca gaccccacag ggcatgaaag
      361 agatatccaa cattcaggtg ggcgatcttg ttctgagcaa caccggctat aacgaggtgc
      421 tgaacgtgtt tccgaaaagc aaaaagaaaa gctataaaat taccctggaa gatggcaaag
      481 aaattatttg tagcgaagaa catctgttcc cgacccagac cggcgaaatg aacatcagcg
      541 gcggtctgaa agagggcatg tgcctgtatg tgaaagaaat gatgctgaaa aagattctga
      601 aaattgaaga gctggatgaa cgcgaactga tcgatattga agtgagcggt aaccatctgt
      661 tttatgcgaa cgacattctg acccacaata gcttcgccga ctgaatcgtt taaacgaatt
      721 cactagtgga tccgagctcg gtaccaagct tgatgcatag cttgagtatt ctaacgcgct
      781 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgagc acgaaagggc
      841 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
      901 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      961 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     1021 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     1081 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     1141 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     1201 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     1261 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     1321 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     1381 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     1441 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     1501 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     1561 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     1621 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     1681 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     1741 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     1801 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     1861 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     1921 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1981 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     2041 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2101 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2161 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     2221 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2281 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2341 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2401 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2461 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2521 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2581 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2641 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2701 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     2761 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     2821 gaagcggaaa ctaggcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     2881 aaataccgca cagatgcgta aggagaaaat accgcatcag gccgcttaat taacccgggc
     2941 cctctagaat tctgcagcat gcttgccgca gccgcc
//
