LOCUS       sequence-242368-        1185 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A BRET-based mScarlet-I-LumiLuc fusion for red-shifted 
            bioluminescence emission.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Yeh HW, Xiong Y, Wu T, Chen M, Ji A, Li X, Ai HW
  TITLE     ATP-Independent Bioluminescent Reporter Variants To Improve in Vivo 
            Imaging.
  JOURNAL   ACS Chem Biol. 2019 Apr 17. doi: 10.1021/acschembio.9b00150.
  PUBMED    30969754
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2019 Apr 17. doi: 10.1021/acschembio.9b00150."
COMMENT     Sequence Label: pcDNA3-LumiScarlet
FEATURES             Location/Qualifiers
     source          1..1185
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(134..152)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     402..421
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggtgagca agggcgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag
       61 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      121 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac
      181 atcctgtccc ctcagttcat gtacggctcc agggccttca tcaagcaccc cgccgacatc
      241 cccgactact ataagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      301 gaggacggcg gcgccgtgac cgtgacccag gacacctccc tggaggacgg caccctgatc
      361 tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag
      421 acaatgggct gggaagcgtc caccgagcgg ttgtaccccg aggacggcgt gctgaagggc
      481 gacattaaga tggccctgcg cctgaaggac ggcggccgct acctggcgga cttcaagacc
      541 acctacaagg ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg
      601 gacatcacct cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgagggc
      661 cgccactcca ccggaaagac tctcggggat tttgttgggg actggcgaca gacagccggc
      721 tacaaccagg ctcaagtcct tgaacaggga ggtttgacca gtttgtttca gaacctcggg
      781 gtgtccgtaa ctccaatcca aaggattgtc ctgagcggtg aaaatgggct gaagatcgat
      841 atccatgtca tcatcccgta tgaaggtctg agctgcgacc aaatggccca gatcgaaaaa
      901 atttttaagg tggtataccc tgtggatgat catcacttta aggcgatcct gcactatggc
      961 acactggtaa tcgacggggt tacgccgaac atgatcgact atttcggaca gccgtatgaa
     1021 ggcatcgcca agttcgacgg caaaaagatc acagtaacag ggaccctgtg gaacggcaac
     1081 acaattatcg acgagcgcct gatcaacccc gacggctccc tgctgttccg agtaaccatt
     1141 aacggagtga ccggctggcg tctgcatgaa cgcattctgg cgtaa
//
