LOCUS       sequence-242376-       10100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre/Lox C-terminal tagging construct encoding 6 copies of the FLAG 
            epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10100)
  AUTHORS   Watson AT1, Garcia V, Bone N, Carr AM, Armstrong J.
  TITLE     Gene tagging and gene replacement using recombinase-mediated 
            cassette exchange in Schizosaccharomyces pombe.
  JOURNAL   Gene. 2008 Jan 15;407(1-2):63-74
  PUBMED    18054176
REFERENCE   2  (bases 1 to 10100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2008-01-15"; volume: "407(1-2)"; pages: "63-7"
COMMENT     Sequence Label: pAW8_6XFLAG
FEATURES             Location/Qualifiers
     source          1..10100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      575..1356
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(1451..1470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1570..1592
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1630..1648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1821..1889,1890..2681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2039..2058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2852..3440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3341..3360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3594..3611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3728..3749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3764..3769,3770..3787,3788..3794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3802..3818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3807..3829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3826..3842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3826..3842
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(3874..3907)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3922..3945
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3952..3975
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3982..4005
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4012..4035
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4042..4065
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4072..4095
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      4125..4312
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4180..4204)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            4387..5743
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        4387..4730
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             4731..5540
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4798..4817)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      5546..5743
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(5838..5855)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5838..5854)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5847..5869)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             8749..9843
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg
       61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
      121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
      181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc
      241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag
      301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc
      361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt
      421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa
      481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg
      541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat
      601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt
      661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag
      721 cgatcagcaa cttattctcc gttgtagatt ctttttcttc ataaattcca acaatgtaac
      781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg
      841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt
      901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc
      961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta
     1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt
     1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca
     1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat
     1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt
     1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact
     1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt
     1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt
     1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg
     3901 aagttatgca tgctcactag tgactacaag gacgacgatg acaagtctag tgactacaag
     3961 gacgacgatg acaagtctag tgactacaag gacgacgatg acaagtctag tgactacaag
     4021 gacgacgatg acaagtctag tgactacaag gacgacgatg acaagtctag tgactacaag
     4081 gacgacgatg acaagtctag atgaggcgcg ccacttctaa ataagcgaat ttcttatgat
     4141 ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg
     4201 actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggt
     4261 tgctttctca ggtatagtat gaggtcgctc ttattgacca cacctctacc ggcagatccg
     4321 ctagggataa cagggtaata tagatctgtt tagcttgcct cgtccccgcc gggtcacccg
     4381 gccagcgaca tggaggccca gaataccctc cttgacagtc ttgacgtgcg cagctcaggg
     4441 gcatgatgtg actgtcgccc gtacatttag cccatacatc cccatgtata atcatttgca
     4501 tccatacatt ttgatggccg cacggcgcga agcaaaaatt acggctcctc gctgcagacc
     4561 tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt gtccccacgc cgcgcccctg
     4621 tagagaaata taaaaggtta ggatttgcca ctgaggttct tctttcatat acttcctttt
     4681 aaaatcttgc taggatacag ttctcacatc acatccgaac ataaacaacc atgggtaagg
     4741 aaaagactca cgtttcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
     4801 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga
     4861 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     4921 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     4981 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggcaaaacag
     5041 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     5101 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     5161 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     5221 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt
     5281 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     5341 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     5401 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     5461 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     5521 tgctcgatga gtttttctaa tcagtactga caataaaaag attcttgttt tcaagaactt
     5581 gtcatttgta tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat
     5641 ttatattttt tttcgcctcg acatcatctg cccagatgcg aagttaagtg cgcagaaagt
     5701 aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga ttcgatacta
     5761 acgccgccat ccagtcgacg aactagtata acttcgtata tggtattata tacgaagtta
     5821 tgcggccgcg cgaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     5881 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     5941 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     6001 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacc atcgtccgga
     6061 tcaatgaagt agatgagttt actacctgta tatacttttt tttttgtcaa agtcactttt
     6121 atggcatttc catgttgaag gattagccac agcactaaat tttcagcgta tgattgcttt
     6181 taaatattta attttcatcg ttttttaatt aatattcaaa acgatttaat gccttctaca
     6241 tctgaagtca agctcataga ctcgggttgg gtagctaaaa ctacatctaa cacgaagggg
     6301 tatagccttc ttaaagtata ctcatgtata aacgttttgg gttcatattt ttgggccacg
     6361 tggtttataa aaattcttaa ctacaccact cggtatcccg cacccgtcta cgtttctacg
     6421 atttcgagct aatattgagt aaagtgatta gcaaaaaaaa taaaagtact cgttgtcgga
     6481 gatcaagaat ttttctatta tctcatctaa accactttct aaaagcgaaa aacaaaatcg
     6541 taatatgcag cttgaatggg cttccatagt ttgaaagaaa aaccctagca gtactggcaa
     6601 gggagacatt ccttttacct ggctaatcgc catcttccag caggcgcacc attgcccctg
     6661 tttcactatc caggttacgg atatagttca tgacaatatt tacattggtc cagccaccag
     6721 cttgcatgat ctccggtatt gaaactccag cgcgggccat atctcgcgcg gctccgacac
     6781 gggcactgtg tccagaccag gccaggtatc tctgaccaga gtcatcctta gcgccgtaaa
     6841 tcaatcgatg agttgcttca aaaatccctt ccagggcgcg agttgatagc tggctggtgg
     6901 cagatggcgc ggcaacacca ttttttctga cccggcaaaa caggtagtta ttcggatcat
     6961 cagctacacc agagacggaa atccatcgct cgaccagttt agttaccccc aggctaagtg
     7021 ccttctctac acctgcggtg ctaaccagcg ttttcgttct gccaatatgg attaacattc
     7081 tcccaccgtc agtacgtgag atatctttaa ccctgatcct ggcaatttcg gctatacgta
     7141 acagggtgtt ataagcaatc cccagaaatg ccagattacg tatatcctgg cagcgatcgc
     7201 tattttccat gagtgaacga acctggtcga aatcagtgcg ttcgaacgct agagcctgtt
     7261 ttgcacgttc accggcatca acgttttctt ttcggatccg ccgcataacc agtgaaacag
     7321 cattgctgtc acttggtcgt ggcagcccgg accgacgatg aagcatgttt agctggccca
     7381 aatgttgctg gatagttttt actgccagac cgcgcgcctg aagatataga agataatcgc
     7441 gaacatcttc aggttctgcg ggaaaccatt tccggttatt caacttgcac catgccgccc
     7501 acgaccggca aacggacaga agcattttcc aggtatgctc agaaaacgcc tggcgatccc
     7561 tgaacatgtc catcaggttc ttgcgaacct catcactcgt tgcatcgacc ggtaactttt
     7621 agattaacat ttgtttaaat atgaaattag gcaatgtaac atactgcagg caaattttgg
     7681 tgtacggtaa gtaaattgga catatgattt aacaaagcga ctataagtca gaaagtgaga
     7741 atgagattga aataattaat tcaacttatt caattgatga tatgccagga ttcctcttcc
     7801 tttatcttca atgtttcgat taacaggtcc attatccggt ttttagtttc gctgcacatt
     7861 gccgaatgac aatcggattc ctttaagctt tgcttttgcc ctgttttctc tccccagtaa
     7921 tcgttgggtg actgaaccat ccagtagccg agagttacgt ttttcctgat ttaataaata
     7981 tccatatcta tttgtctcgt ggtgttttac agcaactaac cgaaattact attttgcatc
     8041 atccaaccat aaagagacaa caataattct agaaaacata taacaagtgt ttttttctat
     8101 ttacgtctca tacgcaaagt ccactttacc atgttttgga aacttgtacc caaccattca
     8161 tttttcgatt ttgtaatgag gctcttttct tctaacaatc attgaggtca ttatcatgtg
     8221 tcctcaccta ttgtttaaat tgaggcttgc gctattccca aaattgtggg tattattttc
     8281 atatatcagt atctgcaatt tcggttcaac tccggaagct cggtacccaa ctgtgggaat
     8341 actcaggtat cgtaagatgc aagagttcga atctcttagc aaccattatt ttttttctct
     8401 caacataacg agaacacaca ggggcgctat cgcacagaat caaattcgat gactggaaat
     8461 tttttgttaa tttcagaggt cgcctgacgc atataccttt ttcaactgaa aaattgggag
     8521 aaaaaggaaa ggtgagagcg ccggaaccgg cttttcatat agaatagaga agcgttcatg
     8581 actaaatgct tgcatcacaa tacttgaagt tgacaatatt atttaaggac ctattgtttt
     8641 ttccaatagg tggttagcaa tcgtcttact ttctaacttt tcttaccttt tacatttcag
     8701 caatatatat atatatatat atatatattt caaggatata ccattgtaat gtctgcccct
     8761 aagaagatcg tcgttttgcc aggtgaccac gttggtcaag aaatcacagc cgaagccatt
     8821 aaggttctta aagctatttc tgatgttcgt tccaatgtca agttcgattt cgaaaatcat
     8881 ttaattggtg gtgctgctat cgatgctaca ggtgttccac ttccagatga ggcgctggaa
     8941 gcctccaaga aggctgatgc cgttttgtta ggtgctgtgg gtggtcctaa atggggtacc
     9001 ggtagtgtta gacctgaaca aggtttacta aaaatccgta aagaacttca attgtacgcc
     9061 aacttaagac catgtaactt tgcatccgac tctcttttag acttatctcc aatcaagcca
     9121 caatttgcta aaggtactga cttcgttgtt gttagagaat tagtgggagg tatttacttt
     9181 ggtaagagaa aggaagacga tggtgatggt gtcgcttggg atagtgaaca atacaccgtt
     9241 ccagaagtgc aaagaatcac aagaatggcc gctttcatgg ccctacaaca tgagccacca
     9301 ttgcctattt ggtccttgga taaagctaat gttttggcct cttcaagatt atggagaaaa
     9361 actgtggagg aaaccatcaa gaacgaattt cctacattga aagttcaaca tcaattgatt
     9421 gattctgccg ccatgatcct agttaagaac ccaacccacc taaatggtat tataatcacc
     9481 agcaacatgt ttggtgatat catctccgat gaagcctccg ttatcccagg ctccttgggt
     9541 ttgttgccat ctgcgtcctt ggcctctttg ccagacaaga acaccgcatt tggtttgtac
     9601 gaaccatgcc atggttccgc tccagatttg ccaaagaata aggtcaaccc tatcgccact
     9661 atcttgtctg ctgcaatgat gttgaaattg tcattgaact tgcctgaaga aggtaaagcc
     9721 attgaagatg cagttaaaaa ggttttggat gcaggtatca gaactggtga tttaggtggt
     9781 tccaacagta ccaccgaagt cggtgatgct gtcgccgaag aagttaagaa aatccttgct
     9841 taaaaagatt ctcttttttt gtgatatttg tacataaact ttataaatga aattcataat
     9901 agaaacgaca cgaaattaca aaatggaata tgttcatagg gtagacgaaa ctatatacgc
     9961 aatctacata catttatcaa gaaggagaaa aaggaggatg taaaggaata caggtaagca
    10021 aattgatact aatggctcaa cgtgataagg aaaaagaatt gcactttaac attaatattg
    10081 acaaggagga gggcatcaca
//
