LOCUS       sequence-242392-        9929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre/Lox C-terminal tagging construct encoding six histidine (His) 
            residues.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9929)
  AUTHORS   Watson AT1, Garcia V, Bone N, Carr AM, Armstrong J.
  TITLE     Gene tagging and gene replacement using recombinase-mediated 
            cassette exchange in Schizosaccharomyces pombe.
  JOURNAL   Gene. 2008 Jan 15;407(1-2):63-74
  PUBMED    18054176
REFERENCE   2  (bases 1 to 9929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2008-01-15"; volume: "407(1-2)"; pages: "63-7"
COMMENT     Sequence Label: pAW8_6XHis
FEATURES             Location/Qualifiers
     source          1..9929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      575..1356
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(1451..1470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1570..1592
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1630..1648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1821..1889,1890..2681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2039..2058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2852..3440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3341..3360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3594..3611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3728..3749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3764..3769,3770..3787,3788..3794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3802..3818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3807..3829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3826..3842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3826..3842
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(3874..3907)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3913..3930
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3954..4141
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4009..4033)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            4216..5572
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        4216..4559
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             4560..5369
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4627..4646)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      5375..5572
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(5667..5684)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5667..5683)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5676..5698)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             8578..9672
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg
       61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
      121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
      181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc
      241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag
      301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc
      361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt
      421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa
      481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg
      541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat
      601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt
      661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag
      721 cgatcagcaa cttattctcc gttgtagatt ctttttcttc ataaattcca acaatgtaac
      781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg
      841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt
      901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc
      961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta
     1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt
     1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca
     1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat
     1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt
     1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact
     1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt
     1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt
     1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg
     3901 aagttatgca tgcatcatca ccatcatcat taaggcgcgc cacttctaaa taagcgaatt
     3961 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat
     4021 tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt
     4081 aggtcaggtt gctttctcag gtatagtatg aggtcgctct tattgaccac acctctaccg
     4141 gcagatccgc tagggataac agggtaatat agatctgttt agcttgcctc gtccccgccg
     4201 ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc
     4261 agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc ccatgtataa
     4321 tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg
     4381 ctgcagacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg tccccacgcc
     4441 gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt ctttcatata
     4501 cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca taaacaacca
     4561 tgggtaagga aaagactcac gtttcgaggc cgcgattaaa ttccaacatg gatgctgatt
     4621 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat
     4681 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
     4741 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga
     4801 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg
     4861 gcaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
     4921 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca
     4981 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg
     5041 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
     5101 ataagctttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
     5161 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
     5221 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     5281 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     5341 ttcatttgat gctcgatgag tttttctaat cagtactgac aataaaaaga ttcttgtttt
     5401 caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt
     5461 tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc
     5521 gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat
     5581 tcgatactaa cgccgccatc cagtcgacga actagtataa cttcgtatat ggtattatat
     5641 acgaagttat gcggccgcgc gaattcactg gccgtcgttt tacaacgtcg tgactgggaa
     5701 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     5761 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     5821 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacca
     5881 tcgtccggat caatgaagta gatgagttta ctacctgtat atactttttt ttttgtcaaa
     5941 gtcactttta tggcatttcc atgttgaagg attagccaca gcactaaatt ttcagcgtat
     6001 gattgctttt aaatatttaa ttttcatcgt tttttaatta atattcaaaa cgatttaatg
     6061 ccttctacat ctgaagtcaa gctcatagac tcgggttggg tagctaaaac tacatctaac
     6121 acgaaggggt atagccttct taaagtatac tcatgtataa acgttttggg ttcatatttt
     6181 tgggccacgt ggtttataaa aattcttaac tacaccactc ggtatcccgc acccgtctac
     6241 gtttctacga tttcgagcta atattgagta aagtgattag caaaaaaaat aaaagtactc
     6301 gttgtcggag atcaagaatt tttctattat ctcatctaaa ccactttcta aaagcgaaaa
     6361 acaaaatcgt aatatgcagc ttgaatgggc ttccatagtt tgaaagaaaa accctagcag
     6421 tactggcaag ggagacattc cttttacctg gctaatcgcc atcttccagc aggcgcacca
     6481 ttgcccctgt ttcactatcc aggttacgga tatagttcat gacaatattt acattggtcc
     6541 agccaccagc ttgcatgatc tccggtattg aaactccagc gcgggccata tctcgcgcgg
     6601 ctccgacacg ggcactgtgt ccagaccagg ccaggtatct ctgaccagag tcatccttag
     6661 cgccgtaaat caatcgatga gttgcttcaa aaatcccttc cagggcgcga gttgatagct
     6721 ggctggtggc agatggcgcg gcaacaccat tttttctgac ccggcaaaac aggtagttat
     6781 tcggatcatc agctacacca gagacggaaa tccatcgctc gaccagttta gttaccccca
     6841 ggctaagtgc cttctctaca cctgcggtgc taaccagcgt tttcgttctg ccaatatgga
     6901 ttaacattct cccaccgtca gtacgtgaga tatctttaac cctgatcctg gcaatttcgg
     6961 ctatacgtaa cagggtgtta taagcaatcc ccagaaatgc cagattacgt atatcctggc
     7021 agcgatcgct attttccatg agtgaacgaa cctggtcgaa atcagtgcgt tcgaacgcta
     7081 gagcctgttt tgcacgttca ccggcatcaa cgttttcttt tcggatccgc cgcataacca
     7141 gtgaaacagc attgctgtca cttggtcgtg gcagcccgga ccgacgatga agcatgttta
     7201 gctggcccaa atgttgctgg atagttttta ctgccagacc gcgcgcctga agatatagaa
     7261 gataatcgcg aacatcttca ggttctgcgg gaaaccattt ccggttattc aacttgcacc
     7321 atgccgccca cgaccggcaa acggacagaa gcattttcca ggtatgctca gaaaacgcct
     7381 ggcgatccct gaacatgtcc atcaggttct tgcgaacctc atcactcgtt gcatcgaccg
     7441 gtaactttta gattaacatt tgtttaaata tgaaattagg caatgtaaca tactgcaggc
     7501 aaattttggt gtacggtaag taaattggac atatgattta acaaagcgac tataagtcag
     7561 aaagtgagaa tgagattgaa ataattaatt caacttattc aattgatgat atgccaggat
     7621 tcctcttcct ttatcttcaa tgtttcgatt aacaggtcca ttatccggtt tttagtttcg
     7681 ctgcacattg ccgaatgaca atcggattcc tttaagcttt gcttttgccc tgttttctct
     7741 ccccagtaat cgttgggtga ctgaaccatc cagtagccga gagttacgtt tttcctgatt
     7801 taataaatat ccatatctat ttgtctcgtg gtgttttaca gcaactaacc gaaattacta
     7861 ttttgcatca tccaaccata aagagacaac aataattcta gaaaacatat aacaagtgtt
     7921 tttttctatt tacgtctcat acgcaaagtc cactttacca tgttttggaa acttgtaccc
     7981 aaccattcat ttttcgattt tgtaatgagg ctcttttctt ctaacaatca ttgaggtcat
     8041 tatcatgtgt cctcacctat tgtttaaatt gaggcttgcg ctattcccaa aattgtgggt
     8101 attattttca tatatcagta tctgcaattt cggttcaact ccggaagctc ggtacccaac
     8161 tgtgggaata ctcaggtatc gtaagatgca agagttcgaa tctcttagca accattattt
     8221 tttttctctc aacataacga gaacacacag gggcgctatc gcacagaatc aaattcgatg
     8281 actggaaatt ttttgttaat ttcagaggtc gcctgacgca tatacctttt tcaactgaaa
     8341 aattgggaga aaaaggaaag gtgagagcgc cggaaccggc ttttcatata gaatagagaa
     8401 gcgttcatga ctaaatgctt gcatcacaat acttgaagtt gacaatatta tttaaggacc
     8461 tattgttttt tccaataggt ggttagcaat cgtcttactt tctaactttt cttacctttt
     8521 acatttcagc aatatatata tatatatata tatatatttc aaggatatac cattgtaatg
     8581 tctgccccta agaagatcgt cgttttgcca ggtgaccacg ttggtcaaga aatcacagcc
     8641 gaagccatta aggttcttaa agctatttct gatgttcgtt ccaatgtcaa gttcgatttc
     8701 gaaaatcatt taattggtgg tgctgctatc gatgctacag gtgttccact tccagatgag
     8761 gcgctggaag cctccaagaa ggctgatgcc gttttgttag gtgctgtggg tggtcctaaa
     8821 tggggtaccg gtagtgttag acctgaacaa ggtttactaa aaatccgtaa agaacttcaa
     8881 ttgtacgcca acttaagacc atgtaacttt gcatccgact ctcttttaga cttatctcca
     8941 atcaagccac aatttgctaa aggtactgac ttcgttgttg ttagagaatt agtgggaggt
     9001 atttactttg gtaagagaaa ggaagacgat ggtgatggtg tcgcttggga tagtgaacaa
     9061 tacaccgttc cagaagtgca aagaatcaca agaatggccg ctttcatggc cctacaacat
     9121 gagccaccat tgcctatttg gtccttggat aaagctaatg ttttggcctc ttcaagatta
     9181 tggagaaaaa ctgtggagga aaccatcaag aacgaatttc ctacattgaa agttcaacat
     9241 caattgattg attctgccgc catgatccta gttaagaacc caacccacct aaatggtatt
     9301 ataatcacca gcaacatgtt tggtgatatc atctccgatg aagcctccgt tatcccaggc
     9361 tccttgggtt tgttgccatc tgcgtccttg gcctctttgc cagacaagaa caccgcattt
     9421 ggtttgtacg aaccatgcca tggttccgct ccagatttgc caaagaataa ggtcaaccct
     9481 atcgccacta tcttgtctgc tgcaatgatg ttgaaattgt cattgaactt gcctgaagaa
     9541 ggtaaagcca ttgaagatgc agttaaaaag gttttggatg caggtatcag aactggtgat
     9601 ttaggtggtt ccaacagtac caccgaagtc ggtgatgctg tcgccgaaga agttaagaaa
     9661 atccttgctt aaaaagattc tctttttttg tgatatttgt acataaactt tataaatgaa
     9721 attcataata gaaacgacac gaaattacaa aatggaatat gttcataggg tagacgaaac
     9781 tatatacgca atctacatac atttatcaag aaggagaaaa aggaggatgt aaaggaatac
     9841 aggtaagcaa attgatacta atggctcaac gtgataagga aaaagaattg cactttaaca
     9901 ttaatattga caaggaggag ggcatcaca
//
