LOCUS       sequence-242396-        5918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Takahashi Y, Morales FC, Kreimann EL, Georgescu MM
  TITLE     PTEN tumor suppressor associates with NHERF proteins to attenuate 
            PDGF receptor signaling.
  JOURNAL   EMBO J. 2006 Feb 22. 25(4):910-20.
  PUBMED    16456542
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2006 Feb 22. 25(4):910-20."
COMMENT     Sequence Label: pCMV-PTEN
FEATURES             Location/Qualifiers
     source          1..5918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             618..641
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    1942..2564
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2141..2160)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2588..2608)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2593..2922
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2773..2908
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2960..2979)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2961..2979)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2993..3009)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2993..3009)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3006..3028)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3017..3033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3041..3047,3048..3065,3066..3071))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3086..3107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3224..3241)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3395..3983)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3475..3494)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4154..4945,4946..5014))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4777..4796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5015..5119)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5146..5601
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5233..5252)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5443..5464
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5727..5749
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5741..5758
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5742..5758
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattgatc taccatggac tacaaagacg atgacgacaa gcttgcggcc gcgaattcat
      661 cgatagatct gatatcggta ccagtcgact ctagaggatc cccggaattc atgacagcca
      721 tcatcaaaga gatcgttagc agaaacaaaa ggagatatca agaggatgga ttcgacttag
      781 acttgaccta tatttatcca aacattattg ctatgggatt tcctgcagaa agacttgaag
      841 gcgtatacag gaacaatatt gatgatgtag taaggttttt ggattcaaag cataaaaacc
      901 attacaagat atacaatctt tgtgctgaaa gacattatga caccgccaaa tttaattgca
      961 gagttgcaca atatcctttt gaagaccata acccaccaca gctagaactt atcaaaccct
     1021 tttgtgaaga tcttgaccaa tggctaagtg aagatgacaa tcatgttgca gcaattcact
     1081 gtaaagctgg aaagggacga actggtgtaa tgatatgtgc atatttatta catcggggca
     1141 aatttttaaa ggcacaagag gccctagatt tctatgggga agtaaggacc agagacaaaa
     1201 agggagtaac tattcccagt cagaggcgct atgtgtatta ttatagctac ctgttaaaga
     1261 atcatctgga ttatagacca gtggcactgt tgtttcacaa gatgatgttt gaaactattc
     1321 caatgttcag tggcggaact tgcaatcctc agtttgtggt ctgccagcta aaggtgaaga
     1381 tatattcctc caattcagga cccacacgac gggaagacaa gttcatgtac tttgagttcc
     1441 ctcagccgtt acctgtgtgt ggtgatatca aagtagagtt cttccacaaa cagaacaaga
     1501 tgctaaaaaa ggacaaaatg tttcactttt gggtaaatac attcttcata ccaggaccag
     1561 aggaaacctc agaaaaagta gaaaatggaa gtctatgtga tcaagaaatc gatagcattt
     1621 gcagtataga gcgtgcagat aatgacaagg aatatctagt acttacttta acaaaaaatg
     1681 atcttgacaa agcaaataaa gacaaagcca accgatactt ttctccaaat tttaaggtga
     1741 agctgtactt cacaaaaaca gtagaggagc cgtcaaatcc agaggctagc agttcaactt
     1801 ctgtaacacc agatgttagt gacaatgaac ctgatcatta tagatattct gacaccactg
     1861 actctgatcc agagaatgaa ccttttgatg aagatcagca tacacaaatt acaaaagtct
     1921 gagtcgactc tagaggatcc cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     1981 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     2041 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     2101 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     2161 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     2221 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     2281 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     2341 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     2401 gctcccttcc ctgtccttct gattttaaaa taactatacc agcaggagga cgtccagaca
     2461 cagcataggc tacctggcca tgcccaaccg gtgggacatt tgagttgctt gcttggcact
     2521 gtcctctcat gcgttgggtc cactcagtag atgcctgttg aattgggtac gcggccagct
     2581 tggctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     2641 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     2701 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     2761 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     2821 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     2881 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctcct cgaggaactg
     2941 aaaaaccaga aagttaattc cctatagtga gtcgtattaa attcgtaatc atgtcatagc
     3001 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     3061 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     3121 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     3181 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     3241 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     3301 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3361 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3421 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3481 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3541 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3601 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3661 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3721 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3781 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3841 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3901 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3961 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     4021 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     4081 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     4141 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     4201 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     4261 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     4321 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4381 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4441 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     4501 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4561 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4621 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4681 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4741 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4801 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4861 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4921 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4981 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     5041 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     5101 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
     5161 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     5221 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     5281 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     5341 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     5401 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5461 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     5521 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     5581 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     5641 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     5701 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
     5761 caagctgatc tatacattga atcaatattg gcaattagcc atattagtca ttggttatat
     5821 agcataaatc aatattggct attggccatt gcatacgttg tatctatatc ataatatgta
     5881 catttatatt ggctcatgtc caatatgacc gccatgtt
//
