LOCUS       sequence-242398-        8760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  orange calcium-modulated bioluminescent indicator, 110nM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Oh Y, Park Y, Cho JH, Wu H, Paulk NK, Liu LX, Kim N, Kay MA, Wu JC, 
            Lin MZ
  TITLE     An orange calcium-modulated bioluminescent indicator for 
            non-invasive activity imaging.
  JOURNAL   Nat Chem Biol. 2019 Apr 1. pii: 10.1038/s41589-019-0256-z. doi: 
            10.1038/s41589-019-0256-z.
  PUBMED    30936501
REFERENCE   2  (bases 1 to 8760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2019 Apr 1. pii: 10.1038/s41589-019-0256-z. doi: 
            10.1038/s41589-019-0256-z."
COMMENT     Sequence Label: pcDNA3.1-Orange_CaMBI_110
FEATURES             Location/Qualifiers
     source          1..8760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        92..395
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        397..670
                     /label=chicken beta-actin promoter
     intron          671..1687
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1611..1633
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1695..1714
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1777..1786
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3322..3399
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             4264..4293
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4309..4326
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4349..4366)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4355..4579
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4625..5053
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4712..4731)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4922..4943
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5062..5082)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5067..5396
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5247..5382
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5309..5328
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5463..6257
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5517..5536)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6127..6146
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6433..6554
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6470..6489)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6524..6543
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6603..6619)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6603..6619)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6616..6638)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6627..6643
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6651..6657,6658..6675,6676..6681))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6696..6717
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6834..6851)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7005..7593)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7085..7104)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7764..8555,8556..8624))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8387..8406
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8625..8729)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatcaatt ctgcaggaat ctagttatta atagtaatca attacggggt
       61 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      121 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      181 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      241 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      301 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      361 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct
      421 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat
      481 tattttgtgc agcgatgggg gcgggggggg ggggcgcgcg ccaggcgggg cggggcgggg
      541 cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
      601 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
      661 gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc
      721 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
      781 cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg
      841 gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg
      901 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg
      961 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag
     1021 cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt
     1081 gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc
     1141 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
     1201 tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
     1261 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg
     1321 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg
     1381 cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg
     1441 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat
     1501 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg
     1561 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
     1621 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt
     1681 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     1741 gatttgatac cgcgggcccg ggatcagctt ggtaccgcca ccatggtgag caagggcgag
     1801 gagctgatca aggagaacat gagaagcaag ctgtacctgg aaggcagcgt gaacggccac
     1861 cagttcaagt gcacccacga aggggagggc aagccctacg agggcaagca gaccaacagg
     1921 atcaaggtgg tggagggagg ccccctgccg ttcgcattcg acatcctggc cacccacttt
     1981 atgtacggga gcaaggtgtt catcaagtac cccgccgacc tccccgatta ttttaagcag
     2041 tccttccctg agggcttcac atgggagaga gtcatggtgt tcgaagacgg gggcgtgctg
     2101 accgccaccc aggacaccag cctccaggac ggcgagctca tctacaacgt caaggtcaga
     2161 ggggtgaact tcccagccaa cggccccgtg atgcagaaga aaacactggg ctgggagccc
     2221 agcaccgaga ccatgtaccc cgctgacggc ggcctggaag gcagatgcga caaggccctg
     2281 aagctcgtgg gcgggggcca cctgcacgtc aacttcaaga ccacatacaa gtccaagaaa
     2341 cccgtgaaga tgcccggcgt ccactacgtg gaccgcagac tggaaagaat caaggaggcc
     2401 gacaacgaga cctacgtcga gcagtacgag cacgctgtgg ccagatactc caacctgggc
     2461 ggaggcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg
     2521 gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta
     2581 actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc
     2641 atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag
     2701 gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta
     2761 atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc
     2821 gtgttcgacg gcaaaaagat cactgtaaca gggaccctga ttatgcatga ccaactgaca
     2881 gaagagcaga ttgcagagtt caaagaagcc ttctcattat tcgacaagga tggggacggc
     2941 accatcacca caaaggaact tggcaccgtt atgaggtcgc ttggacaaaa cccaacggaa
     3001 gcagaattgc aggatatgat caatgaagtc gatgctgatg gcaatggaac gatttacttt
     3061 cctgaatttc ttactatgat ggctagaaaa atgaaggaca cagacagcga agaggaaatc
     3121 cgagaagcat tccgtgtttt tgacaaggat gggaacggct acatcagcgc tgctcagtta
     3181 cgtcacgtca tgacaaacct cggggagaag ttaacagatg aagaagttga tgaaatgata
     3241 agggaagcag atatcgatgg tgatggccaa gtaaactatg aagagtttgt acaaatgatg
     3301 acagcaaagg gtggcgggtc aaagaggcgc tggaagaaaa acttcattgc cgtcagcgct
     3361 gccaaccggt tcaagaagat ctccagctcc ggggcactgg agctctggaa cggcaacaaa
     3421 attatcgacg agcgcctgat caaccccgac ggctccctgc tgttccgagt aaccatcaac
     3481 ggagtgaccg gctggcggct gtgcgaacgc attctggcga ggcacgaact cattaaagaa
     3541 aatatgcggt ccaagctcta tctcgaggga agtgtgaatg gtcatcaatt taagtgtact
     3601 catgagggcg aaggtaaacc ttacgaagga aagcaaacga atcgaataaa ggtcgtggaa
     3661 ggcggaccgc tcccttttgc cttcgatatt ctggctacac atttcatgta tggatctaaa
     3721 gtctttatta agtatcctgc ggatctgcca gactacttta agcagagctt tccagaagga
     3781 tttacttggg aacgagtgat ggtctttgag gatggaggtg tcctcacagc tacgcaagat
     3841 acttcccttc aagatggaga actgatttac aacgtgaaag tgcgcggtgt caattttcct
     3901 gcaaatggtc cagtcatgca aaagaagacc ctcggatggg aaccttctac agaaacaatg
     3961 tatcctgcag atggaggtct cgagggtcgg tgtgataagg ctcttaaact ggtcggagga
     4021 ggtcatctcc atgtgaattt caaaactacc tataaaagca aaaagcctgt caaaatgcca
     4081 ggtgtacatt atgtcgatcg acggctcgag cggataaagg aagctgataa tgaaacgtat
     4141 gtggaacagt atgaacatgc cgtcgcacgg tatagtaatc tcggtggcgg aatggatgaa
     4201 ctctataagt aatgaattct gcagatatcc agcacagtgg cggccgctcg aggtcaccca
     4261 ttcgaacaaa aactcatctc agaagaggat ctgaatatgc ataccggtca tcatcaccat
     4321 caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     4381 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     4441 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     4501 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     4561 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     4621 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4681 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4741 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4801 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4861 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4921 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4981 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     5041 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     5101 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     5161 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5221 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     5281 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     5341 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     5401 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
     5461 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     5521 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     5581 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     5641 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     5701 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     5761 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     5821 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     5881 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     5941 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     6001 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     6061 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     6121 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     6181 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     6241 ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa tgaccgacca agcgacgccc
     6301 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     6361 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     6421 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6481 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6541 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     6601 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6661 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6721 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6781 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6841 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6901 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6961 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7021 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7081 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7141 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7201 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7261 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7321 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7381 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7441 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7501 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7561 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7621 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7681 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7741 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7801 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7861 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7921 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7981 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8041 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8101 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8161 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8221 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8281 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8341 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8401 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8461 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8521 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8581 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8641 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8701 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
