LOCUS       sequence-242404-        8899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human expression plasmid for AsCpf1-NLS-3xHA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8899)
  AUTHORS   Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, Welch MM, Lopez JM, 
            McCaw ZR, Aryee MJ, Joung JK
  TITLE     Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human 
            cells.
  JOURNAL   Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620.
  PUBMED    27347757
REFERENCE   2  (bases 1 to 8899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620."
COMMENT     Sequence Label: SQT1659
FEATURES             Location/Qualifiers
     source          1..8899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..656
                     /label=chicken beta-actin promoter
     intron          657..1673
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1597..1619
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1681..1700
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1772..5692
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      5412..5421
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5693..5740
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5747..5827
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5917..5936)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5982..6037
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6036..6055)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6398..6414)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6398..6414)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6411..6433)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6422..6438
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6446..6452,6453..6470,6471..6476))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6491..6512
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6571..6766
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6617..6752
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6772..6906
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6809..6828)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6863..6882
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6974..6991)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7145..7733)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7225..7244)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7904..8695,8696..8764))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8527..8546
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8765..8869)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggn cgcgcgccag gcggggcggg gcggggcgag gggcggggcg
      541 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
      601 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      661 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      721 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      781 gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc
      841 ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt
      901 gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg
      961 ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg
     1021 gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc
     1141 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg
     1201 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
     1261 cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct
     1441 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
     1501 ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg
     1561 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1621 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     1681 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattctgc agtcgacggt
     1741 accgcgggcc cgggatccac cggtcgccac catgacacag ttcgagggct ttaccaacct
     1801 gtatcaggtg agcaagacac tgcggtttga gctgatccca cagggcaaga ccctgaagca
     1861 catccaggag cagggcttca tcgaggagga caaggcccgc aatgatcact acaaggagct
     1921 gaagcccatc atcgatcgga tctacaagac ctatgccgac cagtgcctgc agctggtgca
     1981 gctggattgg gagaacctga gcgccgccat cgactcctat agaaaggaga aaaccgagga
     2041 gacaaggaac gccctgatcg aggagcaggc cacatatcgc aatgccatcc acgactactt
     2101 catcggccgg acagacaacc tgaccgatgc catcaataag agacacgccg agatctacaa
     2161 gggcctgttc aaggccgagc tgtttaatgg caaggtgctg aagcagctgg gcaccgtgac
     2221 cacaaccgag cacgagaacg ccctgctgcg gagcttcgac aagtttacaa cctacttctc
     2281 cggcttttat gagaacagga agaacgtgtt cagcgccgag gatatcagca cagccatccc
     2341 acaccgcatc gtgcaggaca acttccccaa gtttaaggag aattgtcaca tcttcacacg
     2401 cctgatcacc gccgtgccca gcctgcggga gcactttgag aacgtgaaga aggccatcgg
     2461 catcttcgtg agcacctcca tcgaggaggt gttttccttc cctttttata accagctgct
     2521 gacacagacc cagatcgacc tgtataacca gctgctggga ggaatctctc gggaggcagg
     2581 caccgagaag atcaagggcc tgaacgaggt gctgaatctg gccatccaga agaatgatga
     2641 gacagcccac atcatcgcct ccctgccaca cagattcatc cccctgttta agcagatcct
     2701 gtccgatagg aacaccctgt ctttcatcct ggaggagttt aagagcgacg aggaagtgat
     2761 ccagtccttc tgcaagtaca agacactgct gagaaacgag aacgtgctgg agacagccga
     2821 ggccctgttt aacgagctga acagcatcga cctgacacac atcttcatca gccacaagaa
     2881 gctggagaca atcagcagcg ccctgtgcga ccactgggat acactgagga atgccctgta
     2941 tgagcggaga atctccgagc tgacaggcaa gatcaccaag tctgccaagg agaaggtgca
     3001 gcgcagcctg aagcacgagg atatcaacct gcaggagatc atctctgccg caggcaagga
     3061 gctgagcgag gccttcaagc agaaaaccag cgagatcctg tcccacgcac acgccgccct
     3121 ggatcagcca ctgcctacaa ccctgaagaa gcaggaggag aaggagatcc tgaagtctca
     3181 gctggacagc ctgctgggcc tgtaccacct gctggactgg tttgccgtgg atgagtccaa
     3241 cgaggtggac cccgagttct ctgcccggct gaccggcatc aagctggaga tggagccttc
     3301 tctgagcttc tacaacaagg ccagaaatta tgccaccaag aagccctact ccgtggagaa
     3361 gttcaagctg aactttcaga tgcctacact ggcctctggc tgggacgtga ataaggagaa
     3421 gaacaatggc gccatcctgt ttgtgaagaa cggcctgtac tatctgggca tcatgccaaa
     3481 gcagaagggc aggtataagg ccctgagctt cgagcccaca gagaaaacca gcgagggctt
     3541 tgataagatg tactatgact acttccctga tgccgccaag atgatcccaa agtgcagcac
     3601 ccagctgaag gccgtgacag cccactttca gacccacaca acccccatcc tgctgtccaa
     3661 caatttcatc gagcctctgg agatcacaaa ggagatctac gacctgaaca atcctgagaa
     3721 ggagccaaag aagtttcaga cagcctacgc caagaaaacc ggcgaccaga agggctacag
     3781 agaggccctg tgcaagtgga tcgacttcac aagggatttt ctgtccaagt ataccaagac
     3841 aacctctatc gatctgtcta gcctgcggcc atcctctcag tataaggacc tgggcgagta
     3901 ctatgccgag ctgaatcccc tgctgtacca catcagcttc cagagaatcg ccgagaagga
     3961 gatcatggat gccgtggaga caggcaagct gtacctgttc cagatctata acaaggactt
     4021 tgccaagggc caccacggca agcctaatct gcacacactg tattggaccg gcctgttttc
     4081 tccagagaac ctggccaaga caagcatcaa gctgaatggc caggccgagc tgttctaccg
     4141 ccctaagtcc aggatgaaga ggatggcaca ccggctggga gagaagatgc tgaacaagaa
     4201 gctgaaggat cagaaaaccc caatccccga caccctgtac caggagctgt acgactatgt
     4261 gaatcacaga ctgtcccacg acctgtctga tgaggccagg gccctgctgc ccaacgtgat
     4321 caccaaggag gtgtctcacg agatcatcaa ggataggcgc tttaccagcg acaagttctt
     4381 tttccacgtg cctatcacac tgaactatca ggccgccaat tccccatcta agttcaacca
     4441 gagggtgaat gcctacctga aggagcaccc cgagacacct atcatcggca tcgatcgggg
     4501 cgagagaaac ctgatctata tcacagtgat cgactccacc ggcaagatcc tggagcagcg
     4561 gagcctgaac accatccagc agtttgatta ccagaagaag ctggacaaca gggagaagga
     4621 gagggtggca gcaaggcagg cctggtctgt ggtgggcaca atcaaggatc tgaagcaggg
     4681 ctatctgagc caggtcatcc acgagatcgt ggacctgatg atccactacc aggccgtggt
     4741 ggtgctggag aacctgaatt tcggctttaa gagcaagagg accggcatcg ccgagaaggc
     4801 cgtgtaccag cagttcgaga agatgctgat cgataagctg aattgcctgg tgctgaagga
     4861 ctatccagca gagaaagtgg gaggcgtgct gaacccatac cagctgacag accagttcac
     4921 ctcctttgcc aagatgggca cccagtctgg cttcctgttt tacgtgcctg ccccatatac
     4981 atctaagatc gatcccctga ccggcttcgt ggaccccttc gtgtggaaaa ccatcaagaa
     5041 tcacgagagc cgcaagcact tcctggaggg cttcgacttt ctgcactacg acgtgaaaac
     5101 cggcgacttc atcctgcact ttaagatgaa cagaaatctg tccttccaga ggggcctgcc
     5161 cggctttatg cctgcatggg atatcgtgtt cgagaagaac gagacacagt ttgacgccaa
     5221 gggcacccct ttcatcgccg gcaagagaat cgtgccagtg atcgagaatc acagattcac
     5281 cggcagatac cgggacctgt atcctgccaa cgagctgatc gccctgctgg aggagaaggg
     5341 catcgtgttc agggatggct ccaacatcct gccaaagctg ctggagaatg acgattctca
     5401 cgccatcgac accatggtgg ccctgatccg cagcgtgctg cagatgcgga actccaatgc
     5461 cgccacaggc gaggactata tcaacagccc cgtgcgcgat ctgaatggcg tgtgcttcga
     5521 ctcccggttt cagaacccag agtggcccat ggacgccgat gccaatggcg cctaccacat
     5581 cgccctgaag ggccagctgc tgctgaatca cctgaaggag agcaaggatc tgaagctgca
     5641 gaacggcatc tccaatcagg actggctggc ctacatccag gagctgcgca acaaaaggcc
     5701 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag ggatcctacc catacgatgt
     5761 tccagattac gcttatccct acgacgtgcc tgattatgca tacccatatg atgtccccga
     5821 ctatgcctaa agcggccgca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     5881 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     5941 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     6001 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     6061 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
     6121 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
     6181 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     6241 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     6301 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
     6361 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     6421 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6481 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6541 agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc
     6601 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6661 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6721 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt
     6781 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6841 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6901 tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6961 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7021 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7081 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7141 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7201 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7261 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7321 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7381 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7441 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7501 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7561 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7621 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7681 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7741 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7801 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7861 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7921 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7981 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8041 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8101 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8161 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8221 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8281 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8341 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8401 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8461 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8521 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8581 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8641 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8701 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8761 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8821 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8881 gaaaagtgcc acctgggtc
//
