LOCUS       sequence-242409-        6725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6725)
  AUTHORS   Sapetschnig A, Koch F, Rischitor G, Mennenga T, Suske G
  TITLE     Complexity of translationally controlled transcription factor Sp3 
            isoform expression.
  JOURNAL   J Biol Chem. 2004 Oct 1. 279(40):42095-105.
  PUBMED    15247228
REFERENCE   2  (bases 1 to 6725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2004 Oct 1. 279(40):42095-105."
COMMENT     Sequence Label: pN3-Sp3FL
FEATURES             Location/Qualifiers
     source          1..6725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    3513..3634
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3550..3569)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3604..3623
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3641..4096)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3778..3799)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3990..4009
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4123..4227
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4229..4586
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4229..4420
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4252..4272)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4437..4572
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4621..5415
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4621..5415
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4675..4694)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5285..5304
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5603..5622)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5647..5694
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6023..6611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6512..6531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attccctttt gtgtttcccg cacagtcaat
      661 caaaatagga aaaaaaaatc cccggaccgc tccggccgtg tccgccgccg cttcccgcat
      721 cctctcccgc cgccgccgcc ttcgctcctc accatgtgta aggcggcggg gagccccgcc
      781 tgaggtgccc taaacacact atgaccgctc ccgaaaagcc cgtgaaacaa gaggaaatgg
      841 ctgccttgga cgtggatagc ggcggcggcg gtggcggcgg cggcggccac ggcgagtatc
      901 tgcagcagca gcaacagcac ggaaacggtg cggtggcggc ggcagcggcg gcccaggaca
      961 ctcagccgtc accgctcgct ctgctggccg ctacctgcag caagataggg ccgccatcgc
     1021 cgggcgacga cgaggaggag gcggccgccg cagccggggc ccccgccgcc gccggagcga
     1081 caggtgattt ggcttctgca cagttaggag gagcaccaaa ccgatgggag gttttgtcag
     1141 ccacacctac aactataaaa gatgaagctg gtaatctagt ccagattcca agtgctgcta
     1201 cttcaagtgg gcagtatgtt cttccccttc agaatttgca gaatcaacaa atattttccg
     1261 ttgcaccagg atcagattca tcaaatggtg cagtgtccag tgttcaatat caagtgatac
     1321 cacagatcca gtcagcagat ggtcagcagg ttcaaattgg tttcacaggc tcttcagata
     1381 atgggggtat aaatcaagaa agcagtcaaa ttcagatcat tcctggctct aatcaaacct
     1441 tacttgcctc tggaacacct tctgctaaca tccagaatct cataccacag actggtcaag
     1501 tccaggttca gggagttgca atcggtggtt catcttttcc tggtcaaacc caagtagttg
     1561 ctaatgtgcc tcttggtctg ccaggaaata ttacgtttgt accaatcaat agtgtcgatc
     1621 tagattcttt gggactctcg ggcagttctc agacaatgac tgcaggcatt aatgccgacg
     1681 gacatttgat aaacacagga caagctatgg atagttcaga caattcagaa aggactggtg
     1741 agcgggtttc tcctgatatt aatgaaacta atactgatac agatttattt gtgccaacat
     1801 cctcttcatc acagttgcct gttacgatag atagtacagg tatattacaa caaaacacaa
     1861 atagcttgac tacatctagt gggcaggttc attcttcaga tcttcaggga aattatatcc
     1921 agtcgcctgt ttctgaagag acacaggcac agaatattca ggtttctaca gcacagcctg
     1981 ttgtacagca tctacaactt caagagtctc agcagccaac cagtcaagcc caaattgtgc
     2041 aaggtattac accacagaca atccatggtg tgcaagccag tggtcaaaat atatcacaac
     2101 aggctttgca aaatcttcag ttgcagctga atcctggaac ctttttaatt caggcacaga
     2161 cagtgacccc ttctggacag gtaacttggc aaacgtttca agtacaaggg gtccagaact
     2221 tgcagaattt gcaaatacag aatactgctg cccaacaaat aactctgacg cctgttcaaa
     2281 ccctcacact tggtcaagtt gcggcaggtg gagccttcac ttcaactcca gttagtctaa
     2341 gcactggtca gttgccaaat ctacaaacag ttacagtgaa ctctatagat tctgctggta
     2401 tacagctaca tccaggagag aatgctgaca gtcctgcaga tattaggatc aaggaagaag
     2461 aacctgatcc tgaagagtgg cagctcagtg gtgattctac cttgaatacc aatgacctaa
     2521 cacacttaag agtacaggtg gtagatgaag aaggggacca acaacatcaa gaaggaaaaa
     2581 gacttcggag ggtagcttgc acctgtccca actgtaaaga aggtggtgga agaggtacca
     2641 atcttgggaa aaagaagcaa cacatttgtc atataccagg atgtggtaaa gtctatggga
     2701 agacctcaca tctgagagct catctgcgtt ggcattctgg agaacgccct tttgtttgta
     2761 actggatgta ctgtggtaaa agatttactc gaagtgatga attacagagg cacagaagaa
     2821 cacatacagg tgagaagaaa tttgtttgtc cagaatgttc aaaacgcttt atgagaagtg
     2881 accaccttgc caaacatatt aaaacacacc agaataaaaa aggtattcac tctagcagta
     2941 cagtgctggc atctgtggaa gctgcgcgag atgatacttt gattactgca ggaggaacaa
     3001 cgcttatcct tgcaaatatt caacaaggtt ctgtttcagg gataggaact gttaatactt
     3061 ccgccaccag caatcaagat atccttacca acactgaaat acctttacag cttgtcacag
     3121 tttctggaaa tgagacaatg gagtaaatat tacacaaata cttattcatt gtggttattt
     3181 ttatacagta gtgagaagaa tattgttcct aagttcttag atatcttttt attgatgtgc
     3241 aaaaattttt ggattgacag taacttggtt atacatgaca ctgaaatgcc ttactttgta
     3301 tgatattcca tagtatatta aaaatggtaa aattgcatgg gttttgtagg tacttttgga
     3361 atctagagtc gacggtaccg cgggcccggg atcggccgcg actctagatc ataatcagcc
     3421 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     3481 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     3541 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     3601 gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt
     3661 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     3721 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     3781 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     3841 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     3901 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     3961 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     4021 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     4081 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4141 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4201 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt
     4261 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4321 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     4381 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     4441 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     4501 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     4561 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc
     4621 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     4681 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     4741 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     4801 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     4861 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     4921 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     4981 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     5041 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     5101 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     5161 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     5221 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     5281 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     5341 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     5401 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     5461 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     5521 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     5581 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
     5641 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac
     5701 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     5761 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     5821 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac
     5881 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5941 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6001 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6061 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca
     6721 tgcat
//
