LOCUS       sequence-242420-        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of ilux for bioluminescence imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Gregor C, Gwosch KC, Sahl SJ, Hell SW
  TITLE     Strongly enhanced bacterial bioluminescence with the ilux operon for
            single-cell imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 
            10.1073/pnas.1715946115. Epub 2018 Jan 16.
  PUBMED    29339494
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1715946115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-01-30- 30"; volume: "115"; issue: "5"; pages: "962-967"
COMMENT     Sequence Label: ilux pQE(-)
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     80..98
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    142..158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             177..188
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
       61 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc acctcgagaa
      121 atcataaaaa atttatttgc tttgtgagcg gataacaatt tcacacagaa ttcattaaag
      181 aggagaaatt aactggatcc atgactaaaa aaatttcatt cattattacc ggccaggttg
      241 aaatctttcc cgaaagcgat gatttagtgc aatccattaa ttttggtgat aatagtgttt
      301 acctgccaat attgaatgac tctcatgtaa aaaacattat tgattgtaat ggaaataacg
      361 aattacggtt gcatgacatt gtcaattttc tctatacggt agggcaaaga tggaaaaatg
      421 atgaatactc aagacgcagg acatacattc gtgacttaaa aaaatatatg ggatattcag
      481 aagaaatggc taagctagag gccaattgga tatctatgat attatgttct aaaggcggcc
      541 tttatgatgt tgtagaaaat gaacttggtt ctcgccatat catggatgaa tggctacctc
      601 aggatgaaag ttatgttcgg gcttttccga aaggtaaatc tgtacatctg ttggcaggta
      661 atgttccatt atctgggatc atgtctatat tacgcgcaat tttaactaag aatcagtgta
      721 ttataaaaac atcgtcaacc gatcctttta ccgctaatgc attagcgtta agttttattg
      781 atgtagaccc taatcatccg ataacgcgct ctttatctgt tatatattgg ccccaccaag
      841 gtgatacatc actcgcaaaa gaaattatgc gacatgcgga tgttattgtc gcttggggag
      901 ggccagatgc gattaattgg gcggtagagc atgcgccatc ttatgctgat gtgattaaat
      961 ttggtcctaa aaagagtctt tgcattatcg ataatcctgt tgatttgacg tctgcagcga
     1021 caggtgcggc tcatgatgtt tgtttttacg atcagcgagc ttgntttt
//
