LOCUS       sequence-242431-        2843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pseudomonas aeruginosa disaggregase ClpG cloned as ClpG-6xHis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Lee C, Franke KB, Kamal SM, Kim H, Lunsdorf H, Jager J, Nimtz M, 
            Trcek J, Jansch L, Bukau B, Mogk A, Romling U
  TITLE     Stand-alone ClpG disaggregase confers superior heat tolerance to 
            bacteria.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Jan 9;115(2):E273-E282. doi: 
            10.1073/pnas.1712051115. Epub 2017 Dec 20.
  PUBMED    29263094
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1712051115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-01-9- 9"; volume: "115"; issue: "2"; pages: "E273-E282"
COMMENT     Sequence Label: pSG019
FEATURES             Location/Qualifiers
     source          1..2843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2823..2840
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ccttccgtgg aaacaggagt cctcccatgg cccaggaact ttgcgctatc tgccacgaac
       61 gtcccgccgt cgcccgcgtc agcctggtac agaacggcca gcgccgcgaa ctcgccctct
      121 gcgaactgca ctaccgccag ttgatgcgcc agcagcgcat gcgctcgccg ctggagtcgc
      181 tgttcggcgg cggcagcccg ttcgacgaga tcttctccgg cttcggcgag cagagcccgg
      241 tcaccccggt gcgcgcccgc gagccggagg cggtggacat cgccgagtac ttcagcaagc
      301 agaccaccga gtacctgcaa cgcgccgcgc aggtcgccgc cgaattcggc aagcgcgaag
      361 tggacaccga gcacctgctc tacgccctgg ccgacgccga cgtggtgcag gcggtgctca
      421 agcagttcgg cctgtcgccg gccgacctca agcagtacat cgaggccaac gccgtgcgcg
      481 gcgccagcaa gggcgaggcg agcgaggaca tgaccatctc gccgcgggtg aagagcgcct
      541 tgcagcatgc cttcgccctg tcccgcgaac tcggccacag ctatgtcggc cccgagcacc
      601 tgttgctcgg cctggcggcg gtgccggaca gcttcgccgg gacgctcctg aagaagtacg
      661 gcctgaccga gcaggcgcta cggcagaaag cggtcaaggt ggtcggcaag ggcgccgagg
      721 acggccgcgt ggacggcccg agcaacactc cgcaactgga caagttcagc cgcgacctga
      781 cccggctggc ccgcgaaggc aagctcgatc cggtgatcgg ccgctcgaag gaagtcgaga
      841 ccaccatcga ggttctcgcc cggcgcaaga agaacaaccc ggtgctgatc ggcgagcccg
      901 gcgtcggcaa gaccgccatc gtcgaaggcc tggcccagcg catggtccag ggcgaggtgc
      961 cggaggtgct gcgcgacaag cgcctggtcg aactgaacat caacgccatg gtcgccggcg
     1021 ccaagtaccg cggcgagttc gaggaacgcc tcaagcaggt gatggacgaa ctgcaggcgg
     1081 cgcagagcga gatcatcctg ttcatcgacg aggtgcacac catcgtcggt gccggccagg
     1141 gcggcggcga aggcgggctg gacgtggcca acgtgctgaa gccggcgatg gcgcgtggcg
     1201 agatgaacct gatcggcgcc accaccctca acgagtacca gaagtacatc gagaaggacg
     1261 ccgcgctgga gcggcgcttc cagccggtgt tcgtccccga gccgacggtg gagcagacca
     1321 tctccatcct ccgtggcctg cgcgacaagc tcgaaggcca ccacaaggtg accatccgcg
     1381 acgaggcctt cgtcgccgcc gccgagctgt ccgatcgcta catcggcaac cgcttcctgc
     1441 cggacaaggc catcgacctg atcgaccagg ccgccgcccg cgtgcgcatc gccagcacct
     1501 cgcggccggc cgagatccag gaactggagg ccgagctggc gcagctcaag cgcgagcagg
     1561 actacgccgc cagccgcaag tggtacgacg aggcgaaggt cttcgagaaa cgcatccagg
     1621 aacgcaagga acacctcgag cagatcaccg agcgctggca gcagacccag gggtcgaaga
     1681 ccgaggaggt gcgggtcgag gacatcgccg agatcatctc caggctcacc ggcatcccgg
     1741 tcaccgaact gaccgccgag gagcgcgaga agctcctgca gatggaagag cgcctgcacc
     1801 agcgggtgat cggccagcag gaagcgatca ccgcggtcag cgacgccgtg cgcctggccc
     1861 gcgccgggct gcgccagggc agccggccga tcgccacctt cctgttcctc ggcccgaccg
     1921 gggtgggcaa gaccgagctg gccaaggccc tggccgaggt ggtgttcggc gacgaagacg
     1981 cgatgatccg catcgacatg agcgaataca tggagcgcca cgcggtgtcc cggctgatcg
     2041 gtgcgccgcc gggctacgtc ggctacgacg agggcggcca gctcaccgag cgggtgcgcc
     2101 ggcggccgta cagcgtgatc ctgctcgatg agatcgagaa ggcccacgcc gacgtcaaca
     2161 acatcctcct gcaggtgttc gacgacggcc gcctgaccga cggcaagggg cgcgtggtgg
     2221 acttcaccaa taccatcatc atcgccacca gcaacctcgg ctccgagctg atcatgaaga
     2281 acgcccaggc cggcgagttc gcccagccgc cggagaagct caagcgcgaa ctgatgacta
     2341 ccctgcgcgg acatttccgc ccggaattcc tcaaccgcct cgacgaggtg atcgtcttcg
     2401 agtcgctgag caaggcgcag atcgaggaca tcgtgcgcct gcaactggag cgggtgaagc
     2461 gcgccgcgca cgctcaggac atctacctgc acatcgacga cagcctggtc ggccacctcg
     2521 ccgaagaggc ctaccagccg gagttcggcg cccgcgagct gaagcggcag atccgccagc
     2581 aactggagac gcgcctggcc acggcgatgc tcaagggcga ggtgaaagag ggcgagacgg
     2641 tcaccttctt ctacgacgcc aaggacggcg tcggctaccg caagggcgcc gcgccgaagc
     2701 cggcggcacg caagaaatcc ggcgccggcg agacgcccaa gggccgcgcg acggcggcga
     2761 ggaagccggc ggcgaagaaa ggcgccgccg ccaagggcaa ggccgacaag ccgaaggcca
     2821 agcatcatca tcatcatcac tga
//
