LOCUS       sequence-242432-        2886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pseudomonas aeruginosa disaggregase ClpG(GI) cloned as 
            ClpG(GI)-6xHis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Lee C, Franke KB, Kamal SM, Kim H, Lunsdorf H, Jager J, Nimtz M, 
            Trcek J, Jansch L, Bukau B, Mogk A, Romling U
  TITLE     Stand-alone ClpG disaggregase confers superior heat tolerance to 
            bacteria.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Jan 9;115(2):E273-E282. doi: 
            10.1073/pnas.1712051115. Epub 2017 Dec 20.
  PUBMED    29263094
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1712051115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-01-9- 9"; volume: "115"; issue: "2"; pages: "E273-E282"
COMMENT     Sequence Label: pSG020
FEATURES             Location/Qualifiers
     source          1..2886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      248..257
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1061..1070
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2866..2883
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctttacagga gcatcagcat ggccagaaaa caatgccaag tctgcggcca gcccgccacg
       61 gtgcgggtgg aagccaatct caatggtcgc cacagcacca tgctgttgtg tgacgatcac
      121 tatcgccaac tggtgcgcca gcaaaagcgc accgtctcac cgctggaagc cttgttcggc
      181 tcgcgcagcg ggctgttcga agacttcctt ggcagcgact tcttccgcat cggtgacgac
      241 gcaccgtcca tggcggccga taccgacgag gtcgtcgatg cctcgttcgg cgaacccgcc
      301 ccggccggta cgggcaccgc gcgccgtcgc ggcagtgggc tcgccagccg tatcagcgaa
      361 cagtccgagg ccctattgca ggaggccgcc cgacacgctg cagagttcgg gcgcgccgaa
      421 gtcgataccg aacacctgct gctggcgcta tccgacagcg acgtggtcaa gaccatcctg
      481 gggcagttca agatcaaggt cgatgacctc aagcgccaga tcgaatccga agccaagcgc
      541 ggcgataagc cgttcgaggg cgagatcggc gtgtcgcccc gggtcaagga cgcgctcagc
      601 cgtgctttcg tggcctccaa cgaactcggc cactcttatg tcgggccgga gcatttcctg
      661 atcgggctcg ccgaggaagg cgaaggtttg gcggccaacc tgctgcgccg ttacggcctc
      721 acgccgcaag cgctgcgcca gcaggtaagc aaggtggtcg gcaaaggggc cgaggatggc
      781 cgcgccgaga cgccgaccaa cacgccggaa ctcgacaagt attcgcgcga cctcaccaag
      841 atggcgcgcg agggcaagct cgatccggtc atcggccgcg cgcaggagat cgagacgacc
      901 atcgaagtgc tggcccggcg caagaagaac aacccggtac tgatcggcga gcccggcgtc
      961 ggcaagaccg ccatcgtcga agggctggcg cagcgcatgg tcgccggcga agtgcccgag
     1021 acgctgcgcg acaagcgtct ggtcgaactc aacatcaacg ccatggtggc aggcgccaag
     1081 taccgcggcg agttcgagga gcgcgtgcag aaggtgctca aggaagtgac cgagcaccag
     1141 ggcgagctga ttctcttcat cgacgaggtg cacaccatcg tcggtgcagg ccagggtggc
     1201 ggcgaaggcg ggctggacgt ggccaacgtg ttcaagccga tgatggcgcg cggcgaactg
     1261 aacctgatcg gcgccaccac gctcaacgag tatcagaagt acatcgagaa ggacgccgcg
     1321 ctggagcgtc gcttccagcc ggtgatggtg cccgagccga cggtagcgca gaccatgatg
     1381 atcctgcgcg gcctgcgtga caccttcgag gcgcaccaca aggtcagcat caccgaggat
     1441 gcgatcatcg ccgccgccga gttgtcggac cgctacatca ccgcgcgctt tttgcctgac
     1501 aaggccatcg acctgctcga ccaggcggcc gcacgcgtga agctgtcggc cacggcccgc
     1561 ccggtggcgg tgcaagagct ggagtccgaa ctgcaccagc tgcggcgtga gcaggactat
     1621 gtggcctcgc gcaagcagta cgacaaggcc gccgagctgg gcaagcacat cgaggccaaa
     1681 gaggccgaac tcaagaagct tgtcgaggaa tgggaacgcg agcgcgcctc gggcagtgcc
     1741 gaagtcaaag ccgagcatgt cgcgcagatc gtctcgcgcc tgaccggcat tccggtcaac
     1801 gagctgacgg tggaagaacg cgagaagctg ctgcatctgg agcagcggct gcacgagcgc
     1861 ctcgtgggcc aggacgaagc ggtgcgcgcg gtggccgatg ccgtgcggtt gtcgcgcgcg
     1921 ggcctgcgcg aaggcagcaa gccggtggcg acttttctgt tcctcgggcc gaccggcgtg
     1981 ggcaagaccg agctcgccaa ggcgctggcc gagtccatct atggcgatga aggtgcgctg
     2041 ctgcgcatcg acatgtccga gtacggggaa cgccataccg tggcacgcct ggtgggggcg
     2101 cctccgggtt acgtcggcta cgacgagggt ggccagctca ccgagaaggt gcggcgcaaa
     2161 ccctacagcg tgttgctgct ggacgagatc gagaaggctc accccgacgt ctacaacatc
     2221 ctgctgcagg tgttcgacga cgggcggctc accgacggca agggccgggt ggtggatttc
     2281 accaatacca tcatcatcgc cacctcgaac ttgggctcgg acatcatcca gcgtcggctg
     2341 aaggcccgtg gcgccgccgg cgaggaatac gagaagacca agggcgaggt gatggacgtg
     2401 ctgcgcggac acttccgccc cgagttcctc aaccgcatcg acgagatcat cgtcttccat
     2461 gcgctgggca aggaggagat ccgccatatc gtcggcctgc agctcgatcg tgtggcccgc
     2521 aacgccgcca gccagggcgt gacgctgacc ttcgatcaga ccttgatcga tcacttcgcg
     2581 gaggaaggct acaagcccga gttcggcgcg cgtgagctca agcggctgat ccgcagcgag
     2641 ttggaaactg ctctggcgcg cgagatgctg ggtggcggta tcggcaaggc cgatcacgcc
     2701 agcgcccgct gggacgacaa ggccgaacgg gtggtcttcg agcgccagga gccacccgcg
     2761 aagccggccg agcctgagaa gcccgatgcc gcgaacgtgg ccgagacgcc gccgagcgac
     2821 gcgagcaagc ctgcgcgcaa gaagaagtca gcgggcggcg aatctcatca tcatcatcat
     2881 cactga
//
