LOCUS       sequence-242462-        7841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Yan Q, Bartz S, Mao M, Li L, Kaelin WG
  TITLE     The hypoxia-inducible factor 2alpha N-terminal and C-terminal 
            transactivation domains cooperate to promote renal tumorigenesis in 
            vivo.
  JOURNAL   Mol Cell Biol. 2007 Mar . 27(6):2092-102.
  PUBMED    17220275
REFERENCE   2  (bases 1 to 7841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2007 Mar . 27(6):2092-102."
COMMENT     Sequence Label: HA-HIF2alpha-P405A/P531A-pBabe-puro
FEATURES             Location/Qualifiers
     source          1..7841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1288..1310
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1390..1416
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             1390..1416
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1396..1422
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     primer_bind     complement(4094..4114)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4099..4428
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4279..4414
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4438..5037
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4438..4457)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4934..4954
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5934..5951)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6105..6693)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6185..6204)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6853..7644,7645..7713))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7476..7495
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7714..7818)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatcgaa gactaagctt
     1381 accatggcct acccctacga cgtgcccgac tacgcctccc tcggatccgc caccatgaca
     1441 gctgacaagg agaagaaaag gagtagctcg gagaggagga aggagaagtc ccgggatgct
     1501 gcgcggtgcc ggcggagcaa ggagacggag gtgttctatg agctggccca tgagctgcct
     1561 ctgccccaca gtgtgagctc ccatctggac aaggcctcca tcatgcgact ggcaatcagc
     1621 ttcctgcgaa cacacaagct cctctcctca gtttgctctg aaaacgagtc cgaagccgaa
     1681 gctgaccagc agatggacaa cttgtacctg aaagccttgg agggtttcat tgccgtggtg
     1741 acccaagatg gcgacatgat ctttctgtca gaaaacatca gcaagttcat gggacttaca
     1801 caggtggagc taacaggaca tagtatcttt gacttcactc atccctgcga ccatgaggag
     1861 attcgtgaga acctgagtct caaaaatggc tctggttttg ggaaaaaaag caaagacatg
     1921 tccacagagc gggacttctt catgaggatg aagtgcacgg tcaccaacag aggccgtact
     1981 gtcaacctca agtcagccac ctggaaggtc ttgcactgca cgggccaggt gaaagtctac
     2041 aacaactgcc ctcctcacaa tagtctgtgt ggctacaagg agcccctgct gtcctgcctc
     2101 atcatcatgt gtgaaccaat ccagcaccca tcccacatgg acatccccct ggatagcaag
     2161 accttcctga gccgccacag catggacatg aagttcacct actgtgatga cagaatcaca
     2221 gaactgattg gttaccaccc tgaggagctg cttggccgct cagcctatga attctaccat
     2281 gcgctagact ccgagaacat gaccaagagt caccagaact tgtgcaccaa gggtcaggta
     2341 gtaagtggcc agtaccggat gctcgcaaag catgggggct acgtgtggct ggagacccag
     2401 gggacggtca tctacaaccc tcgcaacctg cagccccagt gcatcatgtg tgtcaactac
     2461 gtcctgagtg agattgagaa gaatgacgtg gtgttctcca tggaccagac tgaatccctg
     2521 ttcaagcccc acctgatggc catgaacagc atctttgata gcagtggcaa gggggctgtg
     2581 tctgagaaga gtaacttcct attcaccaag ctaaaggagg agcccgagga gctggcccag
     2641 ctggctgcta ccccaggaga cgccatcatc tctctggatt tcgggaatca gaacttcgag
     2701 gagtcctcag cctatggcaa ggccatcctg cccccgagcc agccatgggc cacggagttg
     2761 aggagccaca gcacccagag cgaggctggg agcctgcctg ccttcaccgt gccccaggca
     2821 gctgccccgg gcagcaccac ccccagtgcc accagcagca gcagcagctg ctccacgccc
     2881 aatagccctg aagactatta cacatctttg gataacgacc tgaagattga agtgattgag
     2941 aagctcttcg ccatggacac agaggccaag gaccaatgca gtacccagac ggatttcaat
     3001 gagctggact tggagacact ggcagcttat atccccatgg acggggaaga cttccagcta
     3061 agccccatct gccccgagga gcggctcttg gcggagaacc cacagtccac cccccagcac
     3121 tgcttcagtg ccatgacaaa catcttccag ccactggccc ctgtagcccc gcacagtccc
     3181 ttcctcctgg acaagtttca gcagcagctg gagagcaaga agacagagcc cgagcaccgg
     3241 cccatgtcct ccatcttctt tgatgccgga agcaaagcat ccctgccacc gtgctgtggc
     3301 caggccagca cccctctctc ttccatgggg ggcagatcca atacccagtg gcccccagat
     3361 ccaccattac attttgggcc cacaaagtgg gccgtcgggg atcagcgcac agagttcttg
     3421 ggagcagcgc cgttggggcc ccctgtctct ccaccccatg tctccacctt caagacaagg
     3481 tctgcaaagg gttttggggc tcgaggccca gacgtgctga gtccggccat ggtagccctc
     3541 tccaacaagc tgaagctgaa gcgacagctg gagtatgaag agcaagcctt ccaggacctg
     3601 agcggggggg acccacctgg tggcagcacc tcacatttga tgtggaaacg gatgaagaac
     3661 ctcaggggtg ggagctgccc tttgatgccg gacaagccac tgagcgcaaa tgtacccaat
     3721 gataagttca cccaaaaccc catgaggggc ctgggccatc ccctgagaca tctgccgctg
     3781 ccacagcctc catctgccat cagtcccggg gagaacagca agagcaggtt ccccccacag
     3841 tgctacgcca cccagtacca ggactacagc ctgtcgtcag cccacaaggt gtcaggcatg
     3901 gcaagccggc tgctcgggcc ctcatttgag tcctacctgc tgcccgaact gaccagatat
     3961 gactgtgagg tgaacgtgcc cgtgctggga agctccacgc tcctgcaagg aggggacctc
     4021 ctcagagccc tggaccaggc cacctgacaa ttcgatatct cgagtaattc gccagcacag
     4081 tggtcgaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     4141 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4201 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4261 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4321 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     4381 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttaccatg
     4441 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     4501 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     4561 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     4621 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     4681 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     4741 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     4801 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     4861 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     4921 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     4981 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
     5041 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcgataaaa
     5101 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt
     5161 ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata
     5221 gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata
     5281 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
     5341 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     5401 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
     5461 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
     5521 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
     5581 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
     5641 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
     5701 tgactacccg tcagcggggt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
     5761 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcacatgca gcatgtatca
     5821 aaattaattt ggtttttttt cttaagtatt tacattaaat ggccatagtt gcattaatga
     5881 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5941 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6001 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6061 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6121 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6181 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6241 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6301 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6361 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6421 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6481 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6541 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6601 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6661 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6721 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6781 aggatcttca cctagatcct tttgcggccg caaatcaatc taaagtatat atgagtaaac
     6841 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6901 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6961 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7021 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7081 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7141 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7201 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7261 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7321 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7381 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7441 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7501 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7561 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7621 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7681 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7741 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7801 acaaataggg gttccgcgca catttccccg aaaagtgcca c
//
