LOCUS       sequence-242463-        9085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter plasmid with Constitutively expressed mCherry and 
            CggR-regulated YFP (eCitrine).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   Monteiro F, Hubmann G, Takhaveev V, Vedelaar SR, Norder J, Hekelaar 
            J, Saldida J, Litsios A, Wijma HJ, Schmidt A, Heinemann M
  TITLE     Measuring glycolytic flux in single yeast cells with an orthogonal 
            synthetic biosensor.
  JOURNAL   Mol Syst Biol. 2019 Dec;15(12):e9071. doi: 10.15252/msb.20199071.
  PUBMED    31885198
REFERENCE   2  (bases 1 to 9085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2019-12"; volume: "15(12):e9071. doi"; pages: " 
            10.15252/msb.20199071"
COMMENT     Sequence Label: pCggRO-reporter
FEATURES             Location/Qualifiers
     source          1..9085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    69..571
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        608..712
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(713..781,782..1573)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(931..950)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1744..2332
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2233..2252
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2486..2503
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2620..2641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2656..2661,2662..2679,2680..2686)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2694..2710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2699..2721
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2718..2734
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2718..2734
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2753..2773
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2755..2773
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2826..2859
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            2914..4270
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        2914..3257
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             3258..4067
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFPPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3325..3344)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      4073..4270
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    4333..4366
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4688..5404
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(5407..5423)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5408..5424)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      5426..5673
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5442..5460)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      complement(5691..5856)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     5777..5801
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(5879..6589)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6157..6176)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6426..6444
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(6602..7000)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             6692..6703
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7032..7065)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7101..7120)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7102..7120)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7130..7147)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7130..7146)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7139..7161)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7287..7742
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7374..7393)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7584..7605
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7873..8676)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8677..8892)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(8916..8935)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9035..9057
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgga tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa
      121 tttgggaatt tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta
      181 aataaagaag gtagaagagt tacggaatga agaaaaaaaa taaacaaagg tttaaaaaat
      241 ttcaacaaaa agcgtacttt acatatatat ttattagaca agaaaagcag attaaataga
      301 tatacattcg attaacgata agtaaaatgt aaaatcacag gattttcgtg tgtggtcttc
      361 tacacagaca agatgaaaca attcggcatt aatacctgag accaggaaga gcaagataaa
      421 aggtagtatt tgttggcgat ccccctagag tcttttacat cttcggaaaa caaaaactat
      481 tttttcttta atttcttttt ttactttcta tttttaattt atatatttat attaaaaaat
      541 ttaaattata attattttta tagcacgtga tgaaaaggac ccaggtggca cttttcgggg
      601 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      661 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
      721 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
      781 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
      841 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
      901 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
      961 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     1021 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     1081 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     1141 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     1201 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     1261 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     1321 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     1381 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     1441 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     1501 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     1561 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     1621 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     1681 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1741 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1801 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga gggtaactgg
     1861 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     1921 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1981 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2041 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2101 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2161 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2221 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2281 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2341 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2401 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     2461 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     2521 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     2581 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     2641 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     2701 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     2761 ccctcactaa agggaacaaa agctgcagct gaagcttcgt acgctgcagg tcgacaaccc
     2821 ttaatataac ttcgtataat gtatgctata cgaagttatt aggtctagag atctgtttag
     2881 ctagcctcgt ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt
     2941 gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc
     3001 atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc
     3061 aaaaattacg gctcctcgct gcagacctgc gagcagggaa acgctcccct cacagacgcg
     3121 ttgaattgtc cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg
     3181 aggttcttct ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca
     3241 tccgaacata aacaaccatg ggtaaggaaa agactcacgt ttcgaggccg cgattaaatt
     3301 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     3361 gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     3421 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     3481 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     3541 tcaccactgc gatccccggc aaaacagcat tccaggtatt agaagaatat cctgattcag
     3601 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     3661 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     3721 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     3781 aagtctggaa agaaatgcat aagcttttgc cattcccacc ggattcagtc gtcactcatg
     3841 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     3901 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     3961 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     4021 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gtactgacaa
     4081 taaaaagatt cttgttttca agaacttgtc atttgtatag tttttttata ttgtagttgt
     4141 tctattttaa tcaaatgtta gcgtgattta tatttttttt cgcctcgaca tcatctgccc
     4201 agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt
     4261 cgctatactg ctgtcgattc gatactaacg ccgccatcca gtgtcgaaaa cgagctctcg
     4321 agaaccctta atataacttc gtataatgta tgctatacga agttattagg gacatcgtaa
     4381 caacaccaaa ccaaaacttg gcgacgggac caggactgtc ttaccgggac gtttactgtc
     4441 gctcctgttg agtgaatcat atatatagaa agcgggtttt tttaaattcc taacgggact
     4501 ttttttgtca gtccgggact ttaattgtcc gaaaaaataa aaaagtgata gggtatatat
     4561 ttttatataa acgaggttaa gtgatcgcgg gactttccct gtcatcgcgg gactaccctt
     4621 gtcatttgag attaaaaact atacttctat agacacacaa acacaaatac acacactaaa
     4681 ttaataaatg tctaaaggtg aagaattatt cactggtgtt gtcccaattt tggttgaatt
     4741 agatggtgat gttaatggtc acaaattttc tgtctccggt gaaggtgaag gtgatgctac
     4801 ttacggtaaa ttgaccttaa aatttatttg tactactggt aaattgccag ttccatggcc
     4861 aaccttagtc actactttag gttatggttt gatgtgtttt gctagatacc cagatcatat
     4921 gaaacaacat gactttttca agtctgccat gccagaaggt tatgttcaag aaagaactat
     4981 ttttttcaaa gatgacggta actacaagac cagagctgaa gtcaagtttg aaggtgatac
     5041 cttagttaat agaatcgaat taaaaggtat tgattttaaa gaagatggta acattttagg
     5101 tcacaaattg gaatacaact ataactctca caatgtttac atcatggctg acaaacaaaa
     5161 gaatggtatc aaagttaact tcaaaattag acacaacatt gaagatggtt ctgttcaatt
     5221 agctgaccat tatcaacaaa atactccaat tggtgatggt ccagtcttgt taccagacaa
     5281 ccattactta tcctatcaat ctgccttatc caaagatcca aacgaaaaga gagaccacat
     5341 ggtcttgtta gaatttgtta ctgctgctgg tattacccat ggtatggatg aattgtacaa
     5401 ataaatcgat accgtcgacc tcgagtcatg taattagtta tgtcacgctt acattcacgc
     5461 cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
     5521 cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt
     5581 cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag
     5641 aaggttttgg gacgctcgaa ggctttaatt tgcggccggt accagcgtac gagcgacctc
     5701 atgctatacc tgagaaagca acctgaccta caggaaagag ttactcaaga ataagaattt
     5761 tcgttttaaa acctaagagt cactttaaaa tttgtataca cttatttttt ttataactta
     5821 tttaataata aaaatcataa atcataagaa attcgcttat ttagaagtgt caacaacgct
     5881 acttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt
     5941 ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg
     6001 cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt
     6061 ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg
     6121 ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt acggggccgt
     6181 cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg tcctgcaggg
     6241 aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg cgctcccact
     6301 tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg tgcttcacgt
     6361 aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag ggcagggggc
     6421 cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg ccctcgccct
     6481 cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc ttgaagcgca
     6541 tgaactcctt gatgatggcc atgttatcct cctcgccctt gctcaccatt tttctagaaa
     6601 acttagatta gattgctatg ctttctttct aatgagcaag aggtaaaaaa agttgtagta
     6661 gagcaagaaa agtgggactg aaacttggga aattgaaggc cgtttattaa cttaaatatc
     6721 gatgggaggt catcgaaaga gaaaaaaatc aaaaaaagaa ttttcaagaa aaagaaacgt
     6781 gataaaaatt tttattgcct ttttcgacga agaaaaagaa acgaggcggt ctcttttttc
     6841 ttttccaaac ctttagtacg ggtaattaac gacaccctag aggaagaaag aggggaaatt
     6901 tagtatgctg tgcttgggtg ttttgaagtg gtacggcgat gcgcggagtc cgagaagatc
     6961 tggaagagta aaagaggagt agaaacgttt cgaagctatg cataggccac tagtggatct
     7021 gatatcacct aataacttcg tatagcatac attatacgaa gttatattaa gggttctcga
     7081 gagctcgggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     7141 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     7201 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     7261 agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg
     7321 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7381 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7441 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7501 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     7561 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     7621 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     7681 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     7741 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catagggtaa
     7801 taactgatat aattaaattg aagctctaat ttgtgagttt agtatacatg catttactta
     7861 taatacagtt ttttagtttt gctggccgca tcttctcaaa tatgcttccc agcctgcttt
     7921 tctgtaacgt tcaccctcta ccttagcatc ccttcccttt gcaaatagtc ctcttccaac
     7981 aataataatg tcagatcctg tagagaccac atcatccacg gttctatact gttgacccaa
     8041 tgcgtctccc ttgtcatcta aacccacacc gggtgtcata atcaaccaat cgtaaccttc
     8101 atctcttcca cccatgtctc tttgagcaat aaagccgata acaaaatctt tgtcgctctt
     8161 cgcaatgtca acagtaccct tagtatattc tccagtagat agggagccct tgcatgacaa
     8221 ttctgctaac atcaaaaggc ctctaggttc ctttgttact tcttctgccg cctgcttcaa
     8281 accgctaaca atacctgggc ccaccacacc gtgtgcattc gtaatgtctg cccattctgc
     8341 tattctgtat acacccgcag agtactgcaa tttgactgta ttaccaatgt cagcaaattt
     8401 tctgtcttcg aagagtaaaa aattgtactt ggcggataat gcctttagcg gcttaactgt
     8461 gccctccatg gaaaaatcag tcaagatatc cacatgtgtt tttagtaaac aaattttggg
     8521 acctaatgct tcaactaact ccagtaattc cttggtggta cgaacatcca atgaagcaca
     8581 caagtttgtt tgcttttcgt gcatgatatt aaatagcttg gcagcaacag gactaggatg
     8641 agtagcagca cgttccttat atgtagcttt cgacatgatt tatcttcgtt tcctgcaggt
     8701 ttttgttctg tgcagttggg ttaagaatac tgggcaattt catgtttctt caacactaca
     8761 tatgcgtata tataccaatc taagtctgtg ctccttcctt cgttcttcct tctgttcgga
     8821 gattaccgaa tcaaaaaaat ttcaaggaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa
     8881 tgatgaattg aaaaggtggt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     8941 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     9001 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     9061 tcaccgtcat caccgaaacg cgcga
//
