LOCUS       sequence-242498-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive, ubiquitous, nuclear-localized GFP expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Tang W, Das A, Pegoraro AF, Han YL, Huang J, Roberts DA, Yang H, 
            Fredberg JJ, Kotton DN, Bi D, Guo M
  TITLE     Collective curvature sensing and fluidity in three-dimensional 
            multicellular systems
  JOURNAL   Nat. Phys. 18, 1371–1378 (2022).
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Phys. 
            18, 1371–1378 (2022)."
COMMENT     Sequence Label: pHAGE-EF1aL-nlsGFP-W
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..27
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(28..30,31..741)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(28..30,31..741)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(70..91)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(331..350)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     678..699
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             742..759
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggccccca agaagaagcg caaggtggtg agcaagggcg aggagctgtt caccggggtg
       61 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      121 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      181 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
      241 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      301 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      361 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      421 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
      481 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
      541 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      601 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
      661 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      721 ggcatggacg agctgtacaa gcatcatcac catcaccatt gac
//
