LOCUS       sequence-242510-        2187 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lineage specific over expression of TFEB in lung alveolar epithelial
            cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2187)
  TITLE     Kotton lab unpublished
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-hSPC-TFEBeGFP-W
FEATURES             Location/Qualifiers
     source          1..2187
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1462..1471
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1468..1470,1471..1473,1474..2187)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1468..1470,1471..1473,1474..2184)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1513..1534)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1774..1793)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2121..2142
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggcgtcac gcatagggtt gcgcatgcag ctcatgcggg agcaggcgca gcaggaggag
       61 caacgggagc gcatgcagca acaggctgtc atgcattaca tgcagcagca gcagcagcag
      121 caacagcagc agctcggagg gccgcccacc ccggccatca atacccccgt ccacttccag
      181 tcgccaccac ctgtgcctgg ggaggtgttg aaggtgcagt cctacctgga gaatcccaca
      241 tcctaccatc tgcagcagtc gcagcatcag aaggtgcggg agtacctgtc cgagacctat
      301 gggaacaagt ttgctgccca catcagccca gcccagggct ctccgaaacc cccaccagcc
      361 gcctccccag gggtgcgagc tggacacgtg ctgtcctcct ccgctggcaa cagtgctccc
      421 aatagcccca tggccatgct gcacattggc tccaaccctg agagggagtt ggatgatgtc
      481 attgacaaca ttatgcgtct ggacgatgtc cttggctaca tcaatcctga aatgcagatg
      541 cccaacacgc tacccctgtc cagcagccac ctgaatgtgt acagcagcga cccccaggtc
      601 acagcctccc tggtgggcgt caccagcagc tcctgccctg cggacctgac ccagaagcga
      661 gagctcacag atgctgagag cagggccctg gccaaggagc ggcagaagaa agacaatcac
      721 aacttaattg aaaggagacg aaggttcaac atcaatgacc gcatcaagga gttgggaatg
      781 ctgatcccca aggccaatga cctggacgtg cgctggaaca agggcaccat cctcaaggcc
      841 tctgtggatt acatccggag gatgcagaag gacctgcaaa agtccaggga gctggagaac
      901 cactctcgcc gcctggagat gaccaacaag cagctctggc tccgtatcca ggagctggag
      961 atgcaggctc gagtgcacgg cctccctacc acctccccgt ccggcatgaa catggctgag
     1021 ctggcccagc aggtggtgaa gcaggagctg cctagcgaag agggcccagg ggaggccctg
     1081 atgctggggg ctgaggtccc tgaccctgag ccactgccag ctctgccccc gcaagccccg
     1141 ctgcccctgc ccacccagcc accgtcccca ttccatcacc tggacttcag ccacagcctg
     1201 agctttgggg gcagggagga cgagggtccc ccgggctacc ccgaacccct ggcgccgggg
     1261 catggctccc cattccccag cctgtccaag aaggatctgg acctcatgct cctggacgac
     1321 tcactgctac cgctggcctc tgatccactt ctgtccacca tgtcccccga ggcctccaag
     1381 gccagcagcc gccggagcag cttcagcatg gaggagggcg atgtgctgaa ggtaccgcgg
     1441 gcccgggatc caccggtcgc ggccgccatg gtgagcaagg gcgaggagct gttcaccggg
     1501 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1561 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1621 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     1681 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1741 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1801 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1861 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1921 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     1981 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     2041 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     2101 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     2161 ctcggcatgg acgagctgta caagtaa
//
