LOCUS       sequence-242513-        2097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lineage specific over expression of a mutated TFEB in lung alveolar 
            epithelial cells, where TFEB is missing the first 30 amino acids of 
            the sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2097)
  TITLE     Kotton lab unpublished
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-hSPC-TFEB(delta30)eGFP-W
FEATURES             Location/Qualifiers
     source          1..2097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1372..1381
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1378..1380,1381..1383,1384..2097)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1378..1380,1381..1383,1384..2094)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1423..1444)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1684..1703)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2031..2052
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggattaca tgcagcagca gcagcagcag caacagcagc agctcggagg gccgcccacc
       61 ccggccatca atacccccgt ccacttccag tcgccaccac ctgtgcctgg ggaggtgttg
      121 aaggtgcagt cctacctgga gaatcccaca tcctaccatc tgcagcagtc gcagcatcag
      181 aaggtgcggg agtacctgtc cgagacctat gggaacaagt ttgctgccca catcagccca
      241 gcccagggct ctccgaaacc cccaccagcc gcctccccag gggtgcgagc tggacacgtg
      301 ctgtcctcct ccgctggcaa cagtgctccc aatagcccca tggccatgct gcacattggc
      361 tccaaccctg agagggagtt ggatgatgtc attgacaaca ttatgcgtct ggacgatgtc
      421 cttggctaca tcaatcctga aatgcagatg cccaacacgc tacccctgtc cagcagccac
      481 ctgaatgtgt acagcagcga cccccaggtc acagcctccc tggtgggcgt caccagcagc
      541 tcctgccctg cggacctgac ccagaagcga gagctcacag atgctgagag cagggccctg
      601 gccaaggagc ggcagaagaa agacaatcac aacttaattg aaaggagacg aaggttcaac
      661 atcaatgacc gcatcaagga gttgggaatg ctgatcccca aggccaatga cctggacgtg
      721 cgctggaaca agggcaccat cctcaaggcc tctgtggatt acatccggag gatgcagaag
      781 gacctgcaaa agtccaggga gctggagaac cactctcgcc gcctggagat gaccaacaag
      841 cagctctggc tccgtatcca ggagctggag atgcaggctc gagtgcacgg cctccctacc
      901 acctccccgt ccggcatgaa catggctgag ctggcccagc aggtggtgaa gcaggagctg
      961 cctagcgaag agggcccagg ggaggccctg atgctggggg ctgaggtccc tgaccctgag
     1021 ccactgccag ctctgccccc gcaagccccg ctgcccctgc ccacccagcc accatcccca
     1081 ttccatcacc tggacttcag ccacagcctg agctttgggg gcagggagga cgagggtccc
     1141 ccgggctacc ccgaacccct ggcgccgggg catggctccc cattccccag cctgtccaag
     1201 aaggatctgg acctcatgct cctggacgac tcactgctac cgctggcctc tgatccactt
     1261 ctgtccacca tgtcccccga ggcctccaag gccagcagcc gccggagcag cttcagcatg
     1321 gaggagggcg atgtgctgaa ggtaccgcgg gcccgggatc caccggtcgc ggccgccatg
     1381 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1441 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1501 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1561 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     1621 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1681 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1741 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1801 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     1861 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     1921 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1981 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     2041 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaa
//
