LOCUS       sequence-24252-e         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  TITLE     T4 Lysozyme Plasmids - Tray 3
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme A98C WT*
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(701..723)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    712..728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cccgctcatt aggcgggctc agatctgctc atgtttgaca gcttatcatc gataagcttc
       61 ggcaataaat tcttgatatg ttttcatatg tttcctttaa atattttaat tagtaattgt
      121 ctattcaagt aattgtgaat atactatcac aattccaaga gaaagtaaac agctttatag
      181 atttttatac gcgtcccaag tgccagttct aaacgttgta atgactcgtt ttgcgcgatt
      241 aggtgtttga ttataccatc tacttttagc taagttaact gctgcttcat cccagcgttt
      301 ttgttgaagc atacgtaaag agttagtaaa tcctgccaca ccggtttctc ccatttggaa
      361 aaccatatta atcaaacaag cgcgacgaac cgcatcaaga gaatcataaa ccggttttaa
      421 tttagcattt ctcagaattc cgcgaacagc agcatcaaca tcctgattaa agagtttttc
      481 agcctcatct tttgtaatta caccattagt attacgccca atagctttat ctaattcaga
      541 tttagcagca ttaagtgatg gactttttgt aagcaaatga ccgatgccaa tagtgtaata
      601 gccttctgtg tctttataga ttttaagtct aanancttca tctatacgta acatttcaaa
      661 tatattcata atacctccta agcatcgatg gatcctgttt cctgtgtgaa attgttatcc
      721 gctcacaa
//
