LOCUS       sequence-242536-        2943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral bicistronic exprression of both human alpha-1 anitrypsin 
            and ZsGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Wilson AA, Kwok LW, Hovav AH, Ohle SJ, Little FF, Fine A, Kotton DN
  TITLE     Sustained expression of alpha1-antitrypsin after transplantation of 
            manipulated hematopoietic stem cells.
  JOURNAL   Am J Respir Cell Mol Biol. 2008 Aug . 39(2):133-41.
  PUBMED    18323534
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Respir
            Cell Mol Biol. 2008 Aug . 39(2):133-41."
COMMENT     Sequence Label: pHAGE-UBC-hAAT-IRES-ZsGreen-W
FEATURES             Location/Qualifiers
     source          1..2943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..399
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     misc_feature    1667..2234
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1684..2236
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1828..1845)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2055..2074
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2248..2943
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggc ggccgcatgc cgtcttctgt
      421 ctcgtggggc atcctcctgc tggcaggcct gtgctgcctg gtccctgtct ccctggctga
      481 ggatccccag ggagatgctg cccagaagac agatacatcc caccatgatc aggatcaccc
      541 aaccttcaac aagatcaccc ccaacctggc tgagttcgcc ttcagcctat accgccagct
      601 ggcacaccag tccaacagca ccaatatctt cttctcccca gtgagcatcg ctacagcctt
      661 tgcaatgctc tccctgggga ccaaggctga cactcacgat gaaatcctgg agggcctgaa
      721 tttcaacctc acggagattc cggaggctca gatccatgaa ggcttccagg aactcctccg
      781 taccctcaac cagccagaca gccagctcca gctgaccacc ggcaatggcc tgttcctcag
      841 cgagggcctg aagctagtgg ataagttttt ggaggatgtt aaaaagttgt accactcaga
      901 agccttcact gtcaacttcg gggacaccga agaggccaag aaacagatca acgattacgt
      961 ggagaagggt actcaaggga aaattgtgga tttggtcaag gagcttgaca gagacacagt
     1021 ttttgctctg gtgaattaca tcttctttaa aggcaaatgg gagagaccct ttgaagtcaa
     1081 ggacaccgag gaagaggact tccacgtgga ccaggtgacc accgtgaagg tgcctatgat
     1141 gaagcgttta ggcatgttta acatccagca ctgtaagaag ctgtccagct gggtgctgct
     1201 gatgaaatac ctgggcaatg ccaccgccat cttcttcctg cctgatgagg ggaaactaca
     1261 gcacctggaa aatgaactca cccacgatat catcaccaag ttcctggaaa atgaagacag
     1321 aaggtctgcc agcttacatt tacccaaact gtccattact ggaacctatg atctgaagag
     1381 cgtcctgggt caactgggca tcactaaggt cttcagcaat ggggctgacc tctccggggt
     1441 cacagaggag gcacccctga agctctccaa ggccgtgcat aaggctgtgc tgaccatcga
     1501 cgagaaaggg actgaagctg ctggggccat gtttttagag gccataccca tgtctatccc
     1561 ccccgaggtc aagttcaaca aaccctttgt cttcttaatg attgaacaaa ataccaagtc
     1621 tcccctcttc atgggaaaag tggtgaatcc cacccaaaaa taaagatccc tccccccccc
     1681 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     1741 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     1801 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     1861 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     1921 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     1981 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     2041 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     2101 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     2161 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     2221 aaacacgatg ataatatggc cacacatatg gcccagtcca agcacggcct gaccaaggag
     2281 atgaccatga agtaccgcat ggagggctgc gtggacggcc acaagttcgt gatcaccggc
     2341 gagggcatcg gctacccctt caagggcaag caggccatca acctgtgcgt ggtggagggc
     2401 ggccccttgc ccttcgccga ggacatcttg tccgccgcct tcatgtacgg caaccgcgtg
     2461 ttcaccgagt acccccagga catcgtcgac tacttcaaga actcctgccc cgccggctac
     2521 acctgggacc gctccttcct gttcgaggac ggcgccgtgt gcatctgcaa cgccgacatc
     2581 accgtgagcg tggaggagaa ctgcatgtac cacgagtcca agttctacgg cgtgaacttc
     2641 cccgccgacg gccccgtgat gaagaagatg accgacaact gggagccctc ctgcgagaag
     2701 atcatccccg tgcccaagca gggcatcttg aagggcgacg tgagcatgta cctgctgctg
     2761 aaggacggtg gccgcttgcg ctgccagttc gacaccgtgt acaaggccaa gtccgtgccc
     2821 cgcaagatgc ccgactggca cttcatccag cacaagctga cccgcgagga ccgcagcgac
     2881 gccaagaacc agaagtggca cctgaccgag cacgccatcg cctccggctc cgccttgccc
     2941 tga
//
