LOCUS       sequence-24266-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  TITLE     T4 Lysozyme Plasmids - Tray 3
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme L99F M102L WT*
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    724..740
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(747..753,754..769,770..774))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 gctactgaaa aagcccgctc attaggcggg ctcagatctg ctcatgtttg acagcttatc
       61 atcgataagc ttcggcaata aattcttgat atgttttcat atgtttcctt taaatatttt
      121 aattagtaat tgtctattca agtaattgtg aatatactat cacaattcca agagaaagta
      181 aacagcttta tagattttta tacgcgtccc aagtgccagt tctaaacgtt gtaatgactc
      241 gttttgcgcg attaggtgtt tgattatacc atctactttt agctaagtta actgctgctt
      301 catcccagcg tttttgttga agcatacgta aagagttagt aaatcctgcc acaccggttt
      361 ctcccatttg gaaaaccaga ttaataaatg cagcgcgacg aaccgcatca agagaatcat
      421 aaaccggttt taatttagca tttctcagaa ttccgcgaac agcagcatca acatcctgat
      481 taaagagttt ttcagcctca tcttttgtaa ttacaccatt agtattacgc ccaatagctt
      541 tatctaattc agatttagca gcattaagtg atggactttt tgtaagcaaa tgaccgatgc
      601 caatagtgta atagccttct gtgtctttat agattttaag tctaagacct tcatctatac
      661 gtaacatttc aaatatattc ataatacctc ctaagcatcg atggatcctg tttcctgtgt
      721 gaattgttat ccgctcacaa tccacacatt atacgagccg atgattaatt gtcacagggg
      781 gangggagta ngctgatccg nttccgtgtg aatgtancgc tccaa
//
