LOCUS       sequence-242668-        8785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  4x 1545 b long scaffolds divided by Zn-dependent self-cleaving 
            DNAzyme cassettes, from compatibility group 10, made by method IV, 
            yields linear 1033 b long scaffolds after Zn incubation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Engelhardt FAS, Praetorius F, Wachauf CH, Bruggenthies G, Kohler F, 
            Kick B, Kadletz KL, Pham PN, Behler KL, Gerling T, Dietz H
  TITLE     Custom-Size, Functional, and Durable DNA Origami with 
            Design-Specific Scaffolds.
  JOURNAL   ACS Nano. 2019 Apr 22. doi: 10.1021/acsnano.9b01025.
  PUBMED    30990672
REFERENCE   2  (bases 1 to 8785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Nano. 
            2019 Apr 22. doi: 10.1021/acsnano.9b01025."
COMMENT     Sequence Label: pre_IV_CS10_1545_v1
FEATURES             Location/Qualifiers
     source          1..8785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6965..7553)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7045..7064)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7724..8515,8516..8584))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8347..8366
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8585..8689)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8757..8775
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
       61 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggcaagct tgtcactccg
      121 gaagttagtt gagctgtcac agaatgtgac gttgaagact tccggcttaa gctctgaaag
      181 ggttctatat ctccaggtag atctgctccc aatgtaacat gctcgggacc tacaaggtgt
      241 caggatcgaa gattgcacga cgatgactta cggacagccg gacgtactcc ctgaacaatg
      301 cgattcgata tacacggtgg tgttctgttt gggccttctg actcaaagcc aagcctggat
      361 aacagtgtcc ttcgggtcct ccgtgttatg gagcacggct gcttagagca ttgccacgga
      421 gcctttgaag gcagcgaggg cgtggcctcc cgaccgcact agcacaacac aggagaggac
      481 cggcgacata cctgggtgga agtttcatcg gaatactcgt caaacgacta acccatcagc
      541 catcgatcgt atgaatatgt ataatccatc cgtacctaga gtcgcgggcc agcatcaacg
      601 catgtggtaa attggttgga tccgcgagca gtaagaaccc ttaaatcaag cttcccggca
      661 cagcgtcagt ggcggatacg cgcgatgtct gccgtgctga agtagggtaa gggccctcca
      721 tgcggacttc ttggtagctc caagagtgga ttccctcgta agttcggcta cgtcatttac
      781 tgcagcctct gcaagcggag gaactccact atgcacttag ggactactga tatcaaattc
      841 gccgccgaaa cccgcaagat ggtgctcttt aactggccaa tacatagccc agtgaaactg
      901 ttcgcatatg gcgaaccttt ctgcgccact tgtttcgcgc acgtttagga gcttattagc
      961 taaatgaacc agttctctgg agtgatacag acttgcggcg tcccgtaaac cgagagtaac
     1021 ttaaccataa agctggtggg tatagaacat atagcgatca gtacggccat ttgacggcaa
     1081 ataccgtctg gctggcaagt cgggctgatg aaatgcctga cagaacttga actgaccaga
     1141 agtgagcaaa ggcggtctca attaacactt tcctatacga cctcctcttc agagggtcat
     1201 gaggagtctc tacctcgcga agggagggaa gccgcagcag accctatcag ggatcataag
     1261 cgcgctattg cgaatttctc cgacagttgt agaggttgct caactagaca ttagtagcag
     1321 gcatgcaggg tgattaaacg ggcatcgcgt taggctttag catggcccat gaagcgtacg
     1381 tggtcagaat aaataaccta tgggcgcctt atgcgcgtgt gatgttagat atgattcacc
     1441 tgtcggtgca acgtaatata actctcgaga cacacataac gtcgcccgcg tctagctgca
     1501 catttcaaga cgctcatgcc agggcaggac gcgactgcgt tgaggctgta tgttggtgaa
     1561 cgcgtatctg taaaggtacg agtcatagga cactaattca tacaacggcg cttgcatact
     1621 ggtctaagac cgaatggaaa cgttactaag gaggcaccct cagacaggtt cgtcctgatc
     1681 tcgcctcgga tgaacgttga agcgttacct gttaggtaac gtagttgagc tgttcatccg
     1741 atagatccgg aagttagttg agctgtcaca gaatgtgacg ttgaagactt ccggcttaag
     1801 ctctgaaagg gttctatatc tccaggtaga tctgctccca atgtaacatg ctcgggacct
     1861 acaaggtgtc aggatcgaag attgcacgac gatgacttac ggacagccgg acgtactccc
     1921 tgaacaatgc gattcgatat acacggtggt gttctgtttg ggccttctga ctcaaagcca
     1981 agcctggata acagtgtcct tcgggtcctc cgtgttatgg agcacggctg cttagagcat
     2041 tgccacggag cctttgaagg cagcgagggc gtggcctccc gaccgcacta gcacaacaca
     2101 ggagaggacc ggcgacatac ctgggtggaa gtttcatcgg aatactcgtc aaacgactaa
     2161 cccatcagcc atcgatcgta tgaatatgta taatccatcc gtacctagag tcgcgggcca
     2221 gcatcaacgc atgtggtaaa ttggttggat ccgcgagcag taagaaccct taaatcaagc
     2281 ttcccggcac agcgtcagtg gcggatacgc gcgatgtctg ccgtgctgaa gtagggtaag
     2341 ggccctccat gcggacttct tggtagctcc aagagtggat tccctcgtaa gttcggctac
     2401 gtcatttact gcagcctctg caagcggagg aactccacta tgcacttagg gactactgat
     2461 atcaaattcg ccgccgaaac ccgcaagatg gtgctcttta actggccaat acatagccca
     2521 gtgaaactgt tcgcatatgg cgaacctttc tgcgccactt gtttcgcgca cgtttaggag
     2581 cttattagct aaatgaacca gttctctgga gtgatacaga cttgcggcgt cccgtaaacc
     2641 gagagtaact taaccataaa gctggtgggt atagaacata tagcgatcag tacggccatt
     2701 tgacggcaaa taccgtctgg ctggcaagtc gggctgatga aatgcctgac agaacttgaa
     2761 ctgaccagaa gtgagcaaag gcggtctcaa ttaacacttt cctatacgac ctcctcttca
     2821 gagggtcatg aggagtctct acctcgcgaa gggagggaag ccgcagcaga ccctatcagg
     2881 gatcataagc gcgctattgc gaatttctcc gacagttgta gaggttgctc aactagacat
     2941 tagtagcagg catgcagggt gattaaacgg gcatcgcgtt aggctttagc atggcccatg
     3001 aagcgtacgt ggtcagaata aataacctat gggcgcctta tgcgcgtgtg atgttagata
     3061 tgattcacct gtcggtgcaa cgtaatataa ctctcgagac acacataacg tcgcccgcgt
     3121 ctagctgcac atttcaagac gctcatgcca gggcaggacg cgactgcgtt gaggctgtat
     3181 gttggtgaac gcgtatctgt aaaggtacga gtcataggac actaattcat acaacggcgc
     3241 ttgcatactg gtctaagacc gaatggaaac gttactaagg aggcaccctc agacaggttc
     3301 gtcctgatct cgcctcggat gaacgttgaa gcgttacctg ttaggtaacg tagttgagct
     3361 gttcatccga tacttccgga agttagttga gctgtcacag aatgtgacgt tgaagacttc
     3421 cggcttaagc tctgaaaggg ttctatatct ccaggtagat ctgctcccaa tgtaacatgc
     3481 tcgggaccta caaggtgtca ggatcgaaga ttgcacgacg atgacttacg gacagccgga
     3541 cgtactccct gaacaatgcg attcgatata cacggtggtg ttctgtttgg gccttctgac
     3601 tcaaagccaa gcctggataa cagtgtcctt cgggtcctcc gtgttatgga gcacggctgc
     3661 ttagagcatt gccacggagc ctttgaaggc agcgagggcg tggcctcccg accgcactag
     3721 cacaacacag gagaggaccg gcgacatacc tgggtggaag tttcatcgga atactcgtca
     3781 aacgactaac ccatcagcca tcgatcgtat gaatatgtat aatccatccg tacctagagt
     3841 cgcgggccag catcaacgca tgtggtaaat tggttggatc cgcgagcagt aagaaccctt
     3901 aaatcaagct tcccggcaca gcgtcagtgg cggatacgcg cgatgtctgc cgtgctgaag
     3961 tagggtaagg gccctccatg cggacttctt ggtagctcca agagtggatt ccctcgtaag
     4021 ttcggctacg tcatttactg cagcctctgc aagcggagga actccactat gcacttaggg
     4081 actactgata tcaaattcgc cgccgaaacc cgcaagatgg tgctctttaa ctggccaata
     4141 catagcccag tgaaactgtt cgcatatggc gaacctttct gcgccacttg tttcgcgcac
     4201 gtttaggagc ttattagcta aatgaaccag ttctctggag tgatacagac ttgcggcgtc
     4261 ccgtaaaccg agagtaactt aaccataaag ctggtgggta tagaacatat agcgatcagt
     4321 acggccattt gacggcaaat accgtctggc tggcaagtcg ggctgatgaa atgcctgaca
     4381 gaacttgaac tgaccagaag tgagcaaagg cggtctcaat taacactttc ctatacgacc
     4441 tcctcttcag agggtcatga ggagtctcta cctcgcgaag ggagggaagc cgcagcagac
     4501 cctatcaggg atcataagcg cgctattgcg aatttctccg acagttgtag aggttgctca
     4561 actagacatt agtagcaggc atgcagggtg attaaacggg catcgcgtta ggctttagca
     4621 tggcccatga agcgtacgtg gtcagaataa ataacctatg ggcgccttat gcgcgtgtga
     4681 tgttagatat gattcacctg tcggtgcaac gtaatataac tctcgagaca cacataacgt
     4741 cgcccgcgtc tagctgcaca tttcaagacg ctcatgccag ggcaggacgc gactgcgttg
     4801 aggctgtatg ttggtgaacg cgtatctgta aaggtacgag tcataggaca ctaattcata
     4861 caacggcgct tgcatactgg tctaagaccg aatggaaacg ttactaagga ggcaccctca
     4921 gacaggttcg tcctgatctc gcctcggatg aacgttgaag cgttacctgt taggtaacgt
     4981 agttgagctg ttcatccgat acgtccggaa gttagttgag ctgtcacaga atgtgacgtt
     5041 gaagacttcc ggcttaagct ctgaaagggt tctatatctc caggtagatc tgctcccaat
     5101 gtaacatgct cgggacctac aaggtgtcag gatcgaagat tgcacgacga tgacttacgg
     5161 acagccggac gtactccctg aacaatgcga ttcgatatac acggtggtgt tctgtttggg
     5221 ccttctgact caaagccaag cctggataac agtgtccttc gggtcctccg tgttatggag
     5281 cacggctgct tagagcattg ccacggagcc tttgaaggca gcgagggcgt ggcctcccga
     5341 ccgcactagc acaacacagg agaggaccgg cgacatacct gggtggaagt ttcatcggaa
     5401 tactcgtcaa acgactaacc catcagccat cgatcgtatg aatatgtata atccatccgt
     5461 acctagagtc gcgggccagc atcaacgcat gtggtaaatt ggttggatcc gcgagcagta
     5521 agaaccctta aatcaagctt cccggcacag cgtcagtggc ggatacgcgc gatgtctgcc
     5581 gtgctgaagt agggtaaggg ccctccatgc ggacttcttg gtagctccaa gagtggattc
     5641 cctcgtaagt tcggctacgt catttactgc agcctctgca agcggaggaa ctccactatg
     5701 cacttaggga ctactgatat caaattcgcc gccgaaaccc gcaagatggt gctctttaac
     5761 tggccaatac atagcccagt gaaactgttc gcatatggcg aacctttctg cgccacttgt
     5821 ttcgcgcacg tttaggagct tattagctaa atgaaccagt tctctggagt gatacagact
     5881 tgcggcgtcc cgtaaaccga gagtaactta accataaagc tggtgggtat agaacatata
     5941 gcgatcagta cggccatttg acggcaaata ccgtctggct ggcaagtcgg gctgatgaaa
     6001 tgcctgacag aacttgaact gaccagaagt gagcaaaggc ggtctcaatt aacactttcc
     6061 tatacgacct cctcttcaga gggtcatgag gagtctctac ctcgcgaagg gagggaagcc
     6121 gcagcagacc ctatcaggga tcataagcgc gctattgcga atttctccga cagttgtaga
     6181 ggttgctcaa ctagacatta gtagcaggca tgcagggtga ttaaacgggc atcgcgttag
     6241 gctttagcat ggcccatgaa gcgtacgtgg tcagaataaa taacctatgg gcgccttatg
     6301 cgcgtgtgat gttagatatg attcacctgt cggtgcaacg taatataact ctcgagacac
     6361 acataacgtc gcccgcgtct agctgcacat ttcaagacgc tcatgccagg gcaggacgcg
     6421 actgcgttga ggctgtatgt tggtgaacgc gtatctgtaa aggtacgagt cataggacac
     6481 taattcatac aacggcgctt gcatactggt ctaagaccga atggaaacgt tactaaggag
     6541 gcaccctcag acaggttcgt cctgatctcg cctcggatga acgttgaagc gttacctgtt
     6601 aggtaacgta gttgagctgt tcatccgaca ttgaccggga gcgccctgta gcggcgcatt
     6661 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6721 gcccgctccc gggatcggaa ttccggccat cgccctgata gacggttttt cgccctttga
     6781 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaaa cgtctagcgg
     6841 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6901 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6961 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7141 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7381 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     7441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7741 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7801 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7861 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7921 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7981 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8041 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8101 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8161 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8221 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8281 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8341 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8401 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8461 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8521 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8581 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8641 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8701 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     8761 ggcgtatcac gaggcagaat ttcag
//
