LOCUS       sequence-24267-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  TITLE     T4 Lysozyme Plasmids - Tray 3
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme L99F M102L V111I WT*
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(716..738)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    727..743
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(751..757,758..773,774..778))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 gctancgnaa aaagcccgct cattaggcgg gctcagatct gctcatgntt tgacagctta
       61 tcatcgataa gcttcggcaa taaattcttg atatgttttc atatgtttcc tttaaatatt
      121 ttaattagta attgtctatt caagtaattg tgaatatact atcacaattc caagagaaag
      181 taaacagctt tatagatttt tatacgcgtc ccaagtgcca gttctaaacg ttgtaatgac
      241 tcgttttgcg cgattaggtg tttgattata ccatctactt ttagctaagt taactgctgc
      301 ttcatcccag cgtttttgtt gaagcatacg taaagagtta gtaaatcctg caataccggt
      361 ttctcccatt tggaaaacca gattaataaa tgcagcgcga cgaaccgcat caagagaatc
      421 ataaaccggt tttaatttag catttctcag aattccgcga acagcagcat caacatcctg
      481 attaaagagt ttttcagcct catcttttgt aattacacca ttagtattac gcccaatagc
      541 tttatctaat tcagatttag cagcattaag tgatggactt tttgtaagca aatgaccgat
      601 gccaatagtg taatagcctt ctgtgtcttt atagatttta agtctaagac cttcatctat
      661 acgtaacatt tcaaatatat tcataatacc tcctaagcat cgatggatcc tgtttcctgt
      721 gtgaaattgt tatccgctca caattccaca cattatacga gccgatgatn aattgtcaca
      781 ggggatgggg agtagctgat ctgntcctgg tgaatgtanc gctccattca ccataa
//
