LOCUS       sequence-242673-        1317 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  1317 b scaffold based on pSB1A3 backbone with RFP gene as insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Engelhardt FAS, Praetorius F, Wachauf CH, Bruggenthies G, Kohler F, 
            Kick B, Kadletz KL, Pham PN, Behler KL, Gerling T, Dietz H
  TITLE     Custom-Size, Functional, and Durable DNA Origami with 
            Design-Specific Scaffolds.
  JOURNAL   ACS Nano. 2019 Apr 22. doi: 10.1021/acsnano.9b01025.
  PUBMED    30990672
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Nano. 
            2019 Apr 22. doi: 10.1021/acsnano.9b01025."
COMMENT     Sequence Label: pre_IV_RFP_1317_v1
FEATURES             Location/Qualifiers
     source          1..1317
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      345..416
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      432..459
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     649..670
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     protein_bind    814..835
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(850..855,856..873,874..880)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    888..904
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             922..933
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc
       61 accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa
      121 ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg
      181 gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa
      241 gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa
      301 taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg
      361 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      421 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatacgtct ctcattgacc
      481 gggagcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
      541 cgctacactt gccagcgccc tagcgcccgc tcccgggatc ggaattccgg ccatcgccct
      601 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
      661 tccaaactgg aacaacactc aaccctatct cgggcaagct tgtcacagag acgcaatacg
      721 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
      781 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
      841 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
      901 acaatttcac acatactaga gaaagaggag aaatactaga tggcttcctc cgaagacgtt
      961 atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc
     1021 gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa
     1081 gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac
     1141 ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc
     1201 ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt
     1261 acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggt
//
