LOCUS       sequence-242708-       12014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of PYL1-SfGFP-Coilin, lentivirus vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12014)
  AUTHORS   Wang H, Xu X, Nguyen CM, Liu Y, Gao Y, Lin X, Daley T, Kipniss NH, 
            La Russa M, Qi LS
  TITLE     CRISPR-Mediated Programmable 3D Genome Positioning and Nuclear 
            Organization.
  JOURNAL   Cell. 2018 Nov 15;175(5):1405-1417.e14. doi: 
            10.1016/j.cell.2018.09.013. Epub 2018 Oct 11.
  PUBMED    30318144
REFERENCE   2  (bases 1 to 12014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.013"; journalName: "Cell"; date: 
            "2018-11-15- 15"; volume: "175"; issue: "5"; pages: "1405-1417.e14"
COMMENT     Sequence Label: pHR-PGK-PYL1-sfGFP-Coilin
FEATURES             Location/Qualifiers
     source          1..12014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(830..850)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        835..1164
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1015..1150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2298..2363
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2493..2513
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2938..3072
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2975..2994)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3029..3048
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             3240..3873
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3920..4045
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4542..4775
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4960..5004
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5289..5406
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5471..5970
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5506..5529)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5903..5922
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6604..7314
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             6604..7314
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(6631..6659)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7254..7274
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    9117..9705
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9170..9190)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9588..9599)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9708..9724)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9709..9725)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9815..10048
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(10073..10091)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10074..10091)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10381..10400)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10614..10633)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10733..10755
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10793..10811)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10879..10983
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10984..11052,11053..11844)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11202..11221)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgtcggcc gattcattaa tgcagctgtg gaatgtgtgt
      841 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      901 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      961 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     1021 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     1081 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     1141 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
     1201 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     1261 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     1321 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     1381 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     1441 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
     1501 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     1561 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     1621 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     1681 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     1741 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
     1801 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     1861 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     1921 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
     1981 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
     2041 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
     2101 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
     2161 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
     2221 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     2281 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     2341 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     2401 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     2461 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     2521 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     2581 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     2641 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
     2701 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
     2761 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
     2821 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     2881 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     2941 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3001 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3061 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     3121 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     3181 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     3241 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     3301 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     3361 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     3421 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     3481 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     3541 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     3601 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     3661 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     3721 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     3781 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     3841 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     3901 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     3961 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     4021 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     4081 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     4141 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     4201 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     4261 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     4321 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     4381 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     4441 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     4501 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     4561 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     4621 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     4681 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     4741 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     4801 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     4861 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     4921 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     4981 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     5041 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     5101 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     5161 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     5221 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
     5281 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     5341 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     5401 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
     5461 gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     5521 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     5581 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     5641 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     5701 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     5761 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     5821 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     5881 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     5941 ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacg cgtctgcagg
     6001 atatcaagct tgcggtacga ctgagtcatg ggtgggggcg cgccaactca agacgaattc
     6061 acccaactct cccaatcaat cgccgagttc cacacgtacc aactcggtaa cggccgttgc
     6121 tcatctctcc tagctcagcg aatccacgcg ccgccggaaa cagtatggtc cgtggtgaga
     6181 cgtttcgata ggccacagat ttacaaacac ttcatcaaaa gctgtaacgt gagtgaagat
     6241 ttcgagatgc gagtgggatg cacgcgcgac gtgaacgtga taagtggatt accggcgaat
     6301 acgtctcgag agagattaga tctgttggac gatgatcgga gagtgactgg gtttagtata
     6361 accggtggtg aacataggct gaggaattat aaatcggtta cgacggttca tagatttgag
     6421 aaagaagaag aagaagaaag gatctggacc gttgttttgg aatcttatgt tgttgatgta
     6481 ccggaaggta attcggagga agatacgaga ttgtttgctg atacggttat tagattgaat
     6541 cttcagaaac ttgcttcgat cactgaagct atgaacgtgg atccaggtgg aggtggaagc
     6601 ggtagcaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
     6661 gatgttaatg ggcacaaatt ttctgtccgt ggagagggtg aaggtgatgc tacaaacgga
     6721 aaactcaccc ttaaatttat ttgcactact ggaaaactac ctgttccgtg gccaacactt
     6781 gtcactactc tgacctatgg tgttcaatgc ttttcccgtt atccggatca catgaaacgg
     6841 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc
     6901 aaagatgacg ggacctacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt
     6961 aatcgtatcg agttaaaggg tattgatttt aaagaagatg gaaacattct tggacacaaa
     7021 ctcgagtaca actttaactc acacaatgta tacatcacgg cagacaaaca aaagaatgga
     7081 atcaaagcta acttcaaaat tcgccacaac gttgaagatg gttccgttca actagcagac
     7141 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     7201 ctgtcgacac aatctgtcct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt
     7261 cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta caaaggtgga
     7321 ggtcggaccg caagcaagat ggcagcttcc gagacggtta ggctacggct tcaatttgat
     7381 tacccgccgc cagctacccc gcactgtacg gccttctggc ttctggtcga cttgaacaga
     7441 tgccgagtcg tcacagatct cattagtctc atccgccagc gcttcggctt cagttctggg
     7501 gccttcctag gcctctacct ggaggggggg ctcttgcccc ccgccgagag cgcgcgcctt
     7561 gtgagagaca acgactgcct cagagttaaa ttagaagaga gaggagttgc tgagaattct
     7621 gtagtcatca gtaatggtga cattaattta tctcttagaa aagcaaagaa gcgggcattt
     7681 cagttagagg agggtgaaga aactgaacca gattgcaaat attcaaagaa gcattggaag
     7741 agtcgagaga acaataacaa taatgagaag gtcttggatc tggaaccaaa agctgtcaca
     7801 gatcagactg tcagcaaaaa aaacaagaga aaaaataaag caacctgtgg cacagtgggt
     7861 gatgataacg aagaggccaa aagaaaatca ccaaagaaaa aggagaaatg tgaatataaa
     7921 aaaaaggcta agaatcccaa gtctccgaaa gtacaggcag tgaaagactg ggccaatcag
     7981 agatgtagtt ctccaaaagg ttctgctaga aacagccttg ttaaagccaa aaggaaaggt
     8041 agtgtaagcg tttgctcaaa agagagtccc agttcctcct cggagtctga gtcttgtgat
     8101 gaatctatca gtgatggtcc cagcaaagtc actttggagg ccagaaattc ctcagagaaa
     8161 ttaccaactg agttatcaaa ggaagaaccc tctaccaaaa atacaactgc agacaaactg
     8221 gctataaaac ttggctttag ccttaccccc agcaagggca agacctctgg aacaacatct
     8281 tccagttcag actctagtgc agagtcagac gaccaatgct tgatgtcatc gagcaccccg
     8341 gagtgtgctg cgggtttctt aaagacagta ggcctttttg caggaagagg tcgtccaggc
     8401 ccagggctgt catcacagac tgcaggtgct gctggatgga ggcgttctgg ctcaaatggt
     8461 ggtggacagg ctcctggtgc ttctcccagt gtgtctctcc ctgctagttt aggaagagga
     8521 tggggtagag aagagaacct tttttcttgg aagggagcta agggacgggg catgcggggg
     8581 agaggtcgag gacgagggca tcctgtttcc tgtgttgtaa atagaagcac tgacaaccag
     8641 aggcaacagc aattaaatga cgtggtaaaa aattcatcta ctattatcca gaatccagta
     8701 gagacaccca agaaggacta tagtctgtta ccactgttag cagctgcccc tcaagttgga
     8761 gaaaagattg catttaagct tttggagcta acatccagtt actctcctga tgtctctgac
     8821 tacgaggaag gaagaatatt aagccacaat ccagagaccc agcaagtaga tatagaaatt
     8881 ctttcatcct tacctgcctt gagagaacct gggaaatttg atttagttta tcacaatgaa
     8941 aatggagctg aggtagtgga gtacgctgtg acacaggaga gcaagatcac tgtattttgg
     9001 aaagagttga ttgacccaag actgattatt gaatctccaa gtaacacatc aagtacagaa
     9061 cctgcctgag tatgacctcc ctgcaggcat gcaagcttga tatcaagctt atcgataatc
     9121 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     9181 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     9241 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     9301 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     9361 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     9421 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     9481 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     9541 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     9601 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     9661 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     9721 ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
     9781 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     9841 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     9901 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     9961 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    10021 ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt
    10081 gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta
    10141 acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat
    10201 ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta
    10261 acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga
    10321 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
    10381 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
    10441 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
    10501 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
    10561 gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc
    10621 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
    10681 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
    10741 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
    10801 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
    10861 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
    10921 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
    10981 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
    11041 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
    11101 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
    11161 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
    11221 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
    11281 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
    11341 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
    11401 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
    11461 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
    11521 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    11581 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    11641 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    11701 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    11761 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    11821 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    11881 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    11941 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    12001 atcaaaggat cttc
//
