LOCUS       sequence-242721-        7776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. malariae protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 7776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Pm_MSP10-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2940..2957
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3009..3064
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3063..3082)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5300..5318)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5386..5490
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5491..5559,5560..6351)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5709..5728)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6522..7110
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7011..7030
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7264..7281
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7398..7419
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7434..7439,7440..7457,7458..7464)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7472..7488
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7477..7499
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7496..7512
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7496..7512
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 acaccaacga catcaaggac aagaaaaaag aggatgtctc caacaaagag aacaacgccg
     1261 tcgacaacga cgaccacgag aacctggcca tcgagggcgg caacggcaac gataacgaca
     1321 acgccttcgc cggcaacaat gccggcaaga tcaacatcca ggacgacgag aacgccgagt
     1381 tcagccccac cggcatcttc aatgccttcg agaagatctt tctgcggaag cggaaggaca
     1441 acatcgacga gagcgacatg cagagcgaca acagcaatga caacaagaag aacaagaaca
     1501 gcctgaacgc cgccgacgac gatgacgagg aagaggacgg cgagcggagc atcaaggatg
     1561 agatctacca cgccttcaac tacgtgaagg acaagttcgt gccattcttc agcagcaaga
     1621 atcaggccat caaggccgag gatgacgaca ccaccaaccc cgccgaggac aacaaggaca
     1681 tcaacaagca gaagcaggaa cccagcgtcg acgacgccgc caagaaggac cctcccgaga
     1741 gcaaagagtc caacagcaaa gaagtgacca gccagaaagg cggcgagagc atggccaaca
     1801 ccgcccctta cagctacgac aacttcttca agaacaaaga aacccggaag cccaagcccg
     1861 aggtgtccga gcacatccag aaaaccctgc tgaaagaggg gaacgatatc aaagagacaa
     1921 cctcccagat cgacaacgtg gtgtacaact tcgagcaggt catcctcaag accaagttct
     1981 acaccaaggc cattaagaac ttcgtgcact tcaagatgta ccacatctgc gagtacagca
     2041 agtgcggcgc caacgcccgg tgctacatcg tggacaagga caaagaagag tgccgctgcc
     2101 gggccaatta cgtgcaggac accagcgtgg actacttcaa gtgcatcccc atgaccacca
     2161 aggactgcaa gaagaagaac ggcaactgcg acaagaatgc cgactgcagc accgacaaga
     2221 acaacaacat ccggtgccag tgcaagcacg actacttcgg cgacggcatc ttttgcgtga
     2281 tgggctctgg cgcgccgtcg acctccatca cggcctataa gagtgagggg gagtcagcgg
     2341 agttctcctt cccactcaac cttggagagg aaagcctgca gggagagttg agatggaagg
     2401 cagagaaggc tccttcttcc cagtcctgga tcaccttctc cctaaagaac caaaaggtgt
     2461 ctgtgcagaa gtctactagc aaccccaagt tccagctgtc cgaaacgctc ccactcaccc
     2521 ttcagatacc ccaggtctcc cttcagtttg ctggttctgg caacctgacc ctgactctgg
     2581 acagagggat actgtatcag gaagtgaacc tggtggtgat gaaagtgact cagcccgaca
     2641 gcaacacttt gacctgtgag gtgatgggac ccacctcacc caagatgaga ctgatcttga
     2701 agcaggagaa tcaggaggcc agggtctcca ggcaggagaa agtgattcaa gtgcaggccc
     2761 ctgaagcagg ggtgtggcaa tgtctactga gtgaaggtga agaggtcaag atggactcca
     2821 agatccaggt tttatccaaa gggttgaatt ccggcagcct gcaccacatc ctggacgccc
     2881 agaagatgct gtggaaccac agggacagga acctgcctcc tctggcccct ctgggccccc
     2941 accaccatca ccaccattga gtctagacac gtgattaatt aaggatcccc cgacctcgac
     3001 ctctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     3061 ctcactcgga aggacatatg ggagggcaaa tcatttggtc gagatccctc ggagatctct
     3121 agctagagga tcgatccccg ccccggacga actaaacctg actacgacat ctctgcccct
     3181 tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc
     3241 ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga
     3301 catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga
     3361 ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct
     3421 cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta
     3481 caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat
     3541 tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg
     3601 gtagtagtat atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa
     3661 gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc
     3721 ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat
     3781 tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt
     3841 cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt
     3901 aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg
     3961 gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt
     4021 gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca
     4081 tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta tggctatggg
     4141 caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga
     4201 ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg
     4261 ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca
     4321 cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg
     4381 ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta
     4441 ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc
     4501 actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg
     4561 attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt
     4621 gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag
     4681 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     4741 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     4801 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     4861 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     4921 tgctatccta atagagatta gggtagtata tgctatccta atttatatct gggtagcata
     4981 tactacccaa atatctggat agcatatgct atcctaatct atatctgggt agcatatgct
     5041 atcctaatct atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     5101 atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt agcataggct
     5161 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     5221 atcctaatct gtatccgggt agcatatgct atcctcatga taagctgtca aacatgagaa
     5281 ttttcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     5341 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5401 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5461 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5521 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5581 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5641 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5701 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     5761 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5821 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5881 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5941 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6001 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     6061 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6121 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6181 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6241 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6301 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6361 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6421 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6481 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6541 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6601 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6661 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6721 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6781 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6841 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6901 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6961 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7021 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7081 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7141 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7201 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7261 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7321 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     7381 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     7441 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     7501 aacagctatg accatgatta cgccaagctc tagctagagg tcgaccaatt ctcatgtttg
     7561 acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg cagaactggt
     7621 aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
     7681 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
     7741 tgtacattta tattggctca tgtccaatat gaccgc
//
