LOCUS       sequence-242728-        8193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. malariae protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8193)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 8193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Pm_MSP3.10-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3357..3374
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3426..3481
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3480..3499)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5717..5735)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5803..5907
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5908..5976,5977..6768)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6126..6145)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6939..7527
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7428..7447
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7681..7698
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7815..7836
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7851..7856,7857..7874,7875..7881)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7889..7905
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7894..7916
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7913..7929
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7913..7929
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 agatgcccag agaggtggac aacaccaaga acatcggcaa catgaagcgg agcctgcccc
     1261 acaacttcta cgtgaagaat atcgccctgc aggacgagga agagaagcgg accctccagg
     1321 tggaccctcc cagccaggaa gaggccagaa gcgtccagac accccagagc gagctgaacc
     1381 ccgatcagaa agaggcccgg acagagaacg gccaggccgg actggaagcc ctgccttctg
     1441 agggccagaa tctgcagggc cggaccctgt ctaccaacgc cgccgtgaac ggcatcatca
     1501 acgagcccaa cgtggacacc caggaagaac accacggcag cacccagaac ctgaaggaca
     1561 aagagcagca gcccgacgcc gagaacagcc ggtccatcgg aaatatcgtg ggagcccagg
     1621 atacccagga actgagccag gaaaaagaga gcaacaccgc catcagcaga ggcgagcaca
     1681 gcgagaaaga gatcaccaaa gagacagcca ccggcaagac cagcaacggc cacaccgaga
     1741 cagccgagat ctctgccgga aaggccctga agcccagcgt gcagcctaat gccctgcctg
     1801 ccgataaggc cgagagaagc ctggaaaccg ccaccggcct gaacaaggat cagcctgcca
     1861 gccctagcgg cgaggaaccc ctgatcaagc ctgaagtggg caccaccctg aacagcaaga
     1921 gccccgagga agatcggggc gaaaaagaaa agggccccga gccccacaga gccagcgagt
     1981 ctgaacagct gccagccgag aagtccaccg accagaaact gagcgtgtcc gagggcgacc
     2041 tgcccagcac actggatgtg aaggccccaa gcggcccaca gaacgaggac ctgagcacac
     2101 aggaacccca gtccgtcgac aacgagcagg gcaaagaaga ggacgaagag gaacgcaagg
     2161 gcgtcgagga acatcaggaa gagagcccca gcagcctgtc cgagaaagtg gacaaccaga
     2221 acatcctgga acccgtgaac ctgccccagg gcaagaccca caacaccgtg gatgaggatg
     2281 agaaagtgat cgaggaaaat gaggaaaagc agaaagaaga agatgaggaa gatatcatca
     2341 aggccgaaga ggtgcagaac atcgtcaaag agattctgca ggaaggggag gaagagggaa
     2401 aagaggaggg cgaggaagag gaagaggacg aggaacaggc cggcgagtac aactcccacg
     2461 gcaagcagga taagaccgag ggcgtgaaca acgagctgct gaaagagaaa gaggaaagca
     2521 cccctcctcc caacgacgcc accctgtaca agtacttcag catggaatac gaggacgaca
     2581 atgaggccaa gaagttcgcc gacgagctga tcaacaccct ggtcaccagc gtgggcggcg
     2641 acaccagcac cgtgaacacc ctgaaggatc tggaaaagga tatgcggcag ttcttcctca
     2701 acattggcgc gccgtcgacc tccatcacgg cctataagag tgagggggag tcagcggagt
     2761 tctccttccc actcaacctt ggagaggaaa gcctgcaggg agagttgaga tggaaggcag
     2821 agaaggctcc ttcttcccag tcctggatca ccttctccct aaagaaccaa aaggtgtctg
     2881 tgcagaagtc tactagcaac cccaagttcc agctgtccga aacgctccca ctcacccttc
     2941 agatacccca ggtctccctt cagtttgctg gttctggcaa cctgaccctg actctggaca
     3001 gagggatact gtatcaggaa gtgaacctgg tggtgatgaa agtgactcag cccgacagca
     3061 acactttgac ctgtgaggtg atgggaccca cctcacccaa gatgagactg atcttgaagc
     3121 aggagaatca ggaggccagg gtctccaggc aggagaaagt gattcaagtg caggcccctg
     3181 aagcaggggt gtggcaatgt ctactgagtg aaggtgaaga ggtcaagatg gactccaaga
     3241 tccaggtttt atccaaaggg ttgaattccg gcagcctgca ccacatcctg gacgcccaga
     3301 agatgctgtg gaaccacagg gacaggaacc tgcctcctct ggcccctctg ggcccccacc
     3361 accatcacca ccattgagtc tagacacgtg attaattaag gatcccccga cctcgacctc
     3421 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     3481 actcggaagg acatatggga gggcaaatca tttggtcgag atccctcgga gatctctagc
     3541 tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct
     3601 tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg
     3661 ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat
     3721 ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt
     3781 tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt
     3841 acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac
     3901 caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag
     3961 caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta
     4021 gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca
     4081 tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca
     4141 tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt
     4201 agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc
     4261 ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag
     4321 gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac
     4381 ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg
     4441 caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg
     4501 ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa
     4561 cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca
     4621 agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg
     4681 acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc
     4741 caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg
     4801 gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata
     4861 acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact
     4921 aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt
     4981 gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt
     5041 ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat
     5101 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc
     5161 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     5221 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     5281 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     5341 tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac
     5401 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc
     5461 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     5521 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
     5581 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     5641 ctaatctgta tccgggtagc atatgctatc ctcatgataa gctgtcaaac atgagaattt
     5701 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     5761 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5821 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5881 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5941 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6001 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6061 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6121 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     6181 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6241 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6301 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6361 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6421 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     6481 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6541 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6601 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6661 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6721 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6781 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6841 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6901 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6961 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7021 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7081 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7141 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7201 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7261 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7321 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7381 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7441 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7501 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7561 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7621 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7681 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     7741 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     7801 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     7861 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     7921 agctatgacc atgattacgc caagctctag ctagaggtcg accaattctc atgtttgaca
     7981 gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag aactggtagg
     8041 tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc attggttata
     8101 tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt
     8161 acatttatat tggctcatgt ccaatatgac cgc
//
