LOCUS       sequence-242729-        7941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. malariae protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7941)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 7941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Pm_MSP7.1-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3105..3122
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3174..3229
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3228..3247)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5465..5483)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5551..5655
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5656..5724,5725..6516)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5874..5893)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6687..7275
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7176..7195
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7429..7446
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7563..7584
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7599..7604,7605..7622,7623..7629)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7637..7653
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7642..7664
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7661..7677
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7661..7677
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 gaaaccagaa ccctcccatc cctctgaacg aggccctgaa cgagaaccag aatgaggccc
     1261 agcacggcaa ccagaaaaac caggacgtgg acgtgctgga aaccctgacc aagaatatcg
     1321 ccaccgacga gttcaacagc ttcaacaacg ccgacgagaa agagcagaaa ggcaaagaaa
     1381 ccatgaagat ctacaagggc gagagcgagg ccaaagagga aacaaacgag gaacccgaga
     1441 agaaagagaa gttcctggaa aacgtggaca gcgtgctgaa gcacaacagc aatgtgtttc
     1501 tgggcaagaa cgccaagggc gtgtccaccg aagtgatcgc ctccgacgtg aacagcgccg
     1561 atggcaccgt gaccagccct agcgtgaagc tgtctaaggg cgtggacgag gtgccagcct
     1621 ctgccggacc agcagataag caggtccgac aggacaaccc cggcaacgac aatgccggca
     1681 atgataaccc cggaaatgat aatcctggga acgacaacac cgacaaggac aatcctggcg
     1741 agaagggcga caccgagggc aagcctgagg aatccagcct gaccgtgccc gaccagagcc
     1801 cacctatcca gcctcaggac agcaccagcg agacactgga ctctgccgcc ctgcggaaca
     1861 accagatcga gggcaatggc cccagatcca ccgtgtccag agaacctcag gtccgccacg
     1921 ccgatcctac cgccgccaca gtgaatagcg cccctagccc caacagcggc atcgagatca
     1981 gcaatggcgc caccagcacc cacaacagcg ccatcaacac catcaagcac ctggaccggc
     2041 tgtacgacga gatcctggcc ggcaccgaga tgacccagag agtgcggcac agccaccgga
     2101 tgagcgagag aaaccacaac tccgccaaca acatcaacac taagtacgag cagttcaaga
     2161 aagagttcaa cgagtacatc atgaatgaga aagaatacga gatgatgaag aacctgatcg
     2221 acaccgtgtt cagcaagggc aaagtgggca cagagaagaa gaacgccctg cacgagctgt
     2281 tcaaaaacgc cctggaaaat gacaacttca aagaacagtt taagaacgtg atgtacggga
     2341 tctactcctt caccaagaga cacagctacc tgcggggcga cgtgaccacc aacaacgagt
     2401 tctacgacaa gttttacgac aacgccgtgt ccctgctgga cacactgctg attggcgcgc
     2461 cgtcgacctc catcacggcc tataagagtg agggggagtc agcggagttc tccttcccac
     2521 tcaaccttgg agaggaaagc ctgcagggag agttgagatg gaaggcagag aaggctcctt
     2581 cttcccagtc ctggatcacc ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta
     2641 ctagcaaccc caagttccag ctgtccgaaa cgctcccact cacccttcag ataccccagg
     2701 tctcccttca gtttgctggt tctggcaacc tgaccctgac tctggacaga gggatactgt
     2761 atcaggaagt gaacctggtg gtgatgaaag tgactcagcc cgacagcaac actttgacct
     2821 gtgaggtgat gggacccacc tcacccaaga tgagactgat cttgaagcag gagaatcagg
     2881 aggccagggt ctccaggcag gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt
     2941 ggcaatgtct actgagtgaa ggtgaagagg tcaagatgga ctccaagatc caggttttat
     3001 ccaaagggtt gaattccggc agcctgcacc acatcctgga cgcccagaag atgctgtgga
     3061 accacaggga caggaacctg cctcctctgg cccctctggg cccccaccac catcaccacc
     3121 attgagtcta gacacgtgat taattaagga tcccccgacc tcgacctctg gctaataaag
     3181 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     3241 atatgggagg gcaaatcatt tggtcgagat ccctcggaga tctctagcta gaggatcgat
     3301 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     3361 gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga
     3421 cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg
     3481 atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     3541 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     3601 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     3661 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     3721 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
     3781 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
     3841 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
     3901 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
     3961 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
     4021 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
     4081 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
     4141 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
     4201 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
     4261 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
     4321 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
     4381 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
     4441 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
     4501 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     4561 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     4621 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     4681 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
     4741 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
     4801 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
     4861 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     4921 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     4981 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     5041 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga
     5101 gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc
     5161 tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     5221 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     5281 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     5341 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     5401 cgggtagcat atgctatcct catgataagc tgtcaaacat gagaattttc ttgaagacga
     5461 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     5521 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5581 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5641 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5701 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5761 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5821 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5881 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     5941 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6001 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6061 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6121 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6181 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6241 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6301 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6361 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6421 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6481 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6541 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6601 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6661 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6721 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6781 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6841 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6901 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6961 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7021 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7081 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7141 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7201 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7261 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7321 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7381 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7441 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7501 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     7561 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     7621 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     7681 gattacgcca agctctagct agaggtcgac caattctcat gtttgacagc ttatcatcgc
     7741 agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct
     7801 atacattgaa tcaatattgg caattagcca tattagtcat tggttatata gcataaatca
     7861 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg
     7921 gctcatgtcc aatatgaccg c
//
