LOCUS       sequence-242730-        7806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. malariae protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Pm_MSP7.4-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2970..2987
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3039..3094
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3093..3112)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5330..5348)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5416..5520
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5521..5589,5590..6381)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5739..5758)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6552..7140
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7041..7060
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7294..7311
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7428..7449
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7464..7469,7470..7487,7488..7494)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7502..7518
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7507..7529
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7526..7542
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7526..7542
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 aggataagta cgaggacgag cagaagaact acaacaacca gcatgtgctg ctgaacatca
     1261 tcaaggacaa gctggaaaag ctgcagggcg gcatcatcac caacaaggtg tccgacaccc
     1321 tgaacaagga cttcaagagc ctgaagaaac ggatggaaga actcgagaag tacgagaaga
     1381 agtacaccga gggcaacatc gagaacatcc tgaagaacga ggccgaggaa agcctgggcg
     1441 acaagaaaca gatcctgggc gtggacgagg atgagtacaa ctgggacttc ctgggccagg
     1501 gcaacaccat gaccaagggc gaggacaacg aggaaggcgc cgacgacaac gaagatgaag
     1561 agggcgaaga ggacggcacc gccctggaac tgaagtctaa gggcgagcag gacacctcca
     1621 tgctggctca gggcgccgag aagatcagca ccgaactcca gggtggcgcc cagcctacca
     1681 gcgagtctag caacgaggtg caggaacaga gcggcggcac cgatgtgcgg accacaagag
     1741 atcctgccgt ggccgaggcc aagacccctc tggaaaattc ccctagcgcc cagagcaaca
     1801 cccctagcac aggcgagaat gaggccaagg ccgccagcca ggaccaggcc aaagaaagac
     1861 agcccgtgga aagcgccctg gtcaatgccg agcacggccc tacccagggc agcaatatcg
     1921 ccacccctaa ggtgcagtac ctggataagc tgttcgacga ctacctgatc gccagcgaca
     1981 aaaagaaaga gatcaacgtc cacaagtacc accggaagta caacgatttc aagaaggcct
     2041 acgagttcac catgaacgat aacgagtaca agatcgtgaa agagctgttt gacttctact
     2101 tcaagaaagg cgaggaagag tccagcaacg ccgccaacct gatcaacctg tttaagaagg
     2161 tgctggacga taacgtgttc cggaaagaat tcgacaacat catgcacggc atctacggct
     2221 tcgccaagcg gtacacctac ctgcggggcg acaggaccaa cgacgagaac gtgtacaaag
     2281 aactgttcaa gaacatgctg agcctgagca acatcgtggg cgcgccgtcg acctccatca
     2341 cggcctataa gagtgagggg gagtcagcgg agttctcctt cccactcaac cttggagagg
     2401 aaagcctgca gggagagttg agatggaagg cagagaaggc tccttcttcc cagtcctgga
     2461 tcaccttctc cctaaagaac caaaaggtgt ctgtgcagaa gtctactagc aaccccaagt
     2521 tccagctgtc cgaaacgctc ccactcaccc ttcagatacc ccaggtctcc cttcagtttg
     2581 ctggttctgg caacctgacc ctgactctgg acagagggat actgtatcag gaagtgaacc
     2641 tggtggtgat gaaagtgact cagcccgaca gcaacacttt gacctgtgag gtgatgggac
     2701 ccacctcacc caagatgaga ctgatcttga agcaggagaa tcaggaggcc agggtctcca
     2761 ggcaggagaa agtgattcaa gtgcaggccc ctgaagcagg ggtgtggcaa tgtctactga
     2821 gtgaaggtga agaggtcaag atggactcca agatccaggt tttatccaaa gggttgaatt
     2881 ccggcagcct gcaccacatc ctggacgccc agaagatgct gtggaaccac agggacagga
     2941 acctgcctcc tctggcccct ctgggccccc accaccatca ccaccattga gtctagacac
     3001 gtgattaatt aaggatcccc cgacctcgac ctctggctaa taaaggaaat ttattttcat
     3061 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     3121 tcatttggtc gagatccctc ggagatctct agctagagga tcgatccccg ccccggacga
     3181 actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc
     3241 agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca
     3301 aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc
     3361 aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat
     3421 tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc
     3481 ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag
     3541 ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt
     3601 aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct
     3661 aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa
     3721 gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg
     3781 ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca
     3841 aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc
     3901 taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg
     3961 tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta
     4021 tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac
     4081 attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat
     4141 gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca atatgatact
     4201 ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc
     4261 tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc
     4321 taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt
     4381 ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc
     4441 tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc
     4501 actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata
     4561 agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca
     4621 cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag
     4681 agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata
     4741 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     4801 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     4861 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     4921 tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata
     4981 tgctatccta atttatatct gggtagcata tactacccaa atatctggat agcatatgct
     5041 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agcataggct
     5101 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     5161 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     5221 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
     5281 atcctcatga taagctgtca aacatgagaa ttttcttgaa gacgaaaggg cctcgtgata
     5341 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     5401 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5461 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5521 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5581 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5641 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5701 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5761 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5821 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5881 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5941 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     6001 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     6061 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     6121 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     6181 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6241 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6301 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6361 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6421 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6481 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6541 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6601 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6661 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6721 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6781 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6841 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6901 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6961 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7021 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7081 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7141 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7201 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7261 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7321 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     7381 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     7441 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     7501 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
     7561 tagctagagg tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
     7621 tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat
     7681 attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc
     7741 cattgcatac gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat
     7801 gaccgc
//
