LOCUS       sequence-242732-        8889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. ovale protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 8889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Po_GAMA-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4053..4070
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4122..4177
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4176..4195)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6413..6431)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6499..6603
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6604..6672,6673..7464)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6822..6841)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7635..8223
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8124..8143
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8377..8394
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8511..8532
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8547..8552,8553..8570,8571..8577)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8585..8601
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8590..8612
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8609..8625
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8609..8625
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccc
     1201 tgatcagaaa ccccaacagc aatagccagg ccctggtgcc cgagcagaac aacaaccaga
     1261 acggcaagcc caacggcctg agcaacaaca atgacaatac ctgcgagatc cagaaaatgg
     1321 ccgaggaaat gatggaaaag ctgctgaacg agaaggacgt gttcaccagc atcatggaac
     1381 ccctgcagag caagctgaat gacgactacc tgtgcaccca gcccaagtac accaacatct
     1441 gcatcaacga gaaagacaag acccctctga ccttcccctg cacctcccct aagtacgaga
     1501 acctgatcca cgagttcacc tacaagaagc tgtgccacag caagatcgcc ttcagcaaca
     1561 ccctgctgaa gtccttcatc gaccagaaca aagaggaaaa caccttcaac aatatcgccc
     1621 agaactacaa taccctgctc aactgcatcg acgacgacct gaaagagatc tacaagagca
     1681 gcatcgacct gttcagcgat ctgaaggaca gcgtggtgga aatcaccgag aaactgtggt
     1741 ccaagaccat caccgacgtg ctgaagaaga gagagcagct ggtcaccggc atcttttgcg
     1801 agctgagaaa cggcaacaag gccagcctgg tcgagaacag cctgagctac gagaatctgg
     1861 gcatcctgaa gatcaacaac gtgcagctgc tcaatgaggc ctacagcgcc ttctccgact
     1921 actactatta cttccccatc ttcgccacca agctgctcga gaagggcggc ctcgtggaac
     1981 ggctggtgtc catccacgaa aacctggcca actaccggac ccggaacatc ctcaagaaga
     2041 ttaacgacca gtccaagaac gaggtgctga acaacgaaga actgctgaat agcctcagca
     2101 actacaagca ccacaatcag aagaacaacg gcgccttcgc ccagctgaag ggcatcaaga
     2161 tctctgccct ggaagccgcc agcaatggca acaacgaggc tgcctctgga tccgctggcg
     2221 gcagagccaa tgagaacatc aatggcgccg acaacaccgc cagaagcgtg aacgccaacg
     2281 aaaacgccgc ccaggcccag aatgagcagc agaatcagcc acagaaccag cagcagaacc
     2341 acgctcagaa ccaggcacag aatcaggccc agaaccaggt gcagaaccag gcccagaatc
     2401 aggctcagaa tcagatgcag aatcacgctc agaatcaggc tcagaaccag gctcagaatc
     2461 aggcacagaa ccaggcccag aaccaggcac agaacgaagc tcagaatgaa gcccagaacg
     2521 aagcacagaa ccagccccag aatcagatgc agaaccaggc tcagaaccag gcccagaacc
     2581 aggtgcagaa tcaggctcag aacgaagccc agaatcagcc tcagaacgag gcacaccacc
     2641 ggcagcagaa tgaggccacc aatctggccg ccagagtgga acagaacaat cacatcagca
     2701 gctccaccaa ccgggtcctg aacaatcccc tgtacagcac cagctccctg aaggccaaga
     2761 atgtggccca gctcgtgaag gatctgctgc agtccatcaa catcgtgaag tttgagaaca
     2821 atgagcccac cggccagatc gacgaagagg gaatcaagaa actgattcag aacagcttcc
     2881 tggacctgag cgataacacc atgctcgtgc ggctgctgat caagccccag gccgtgatcc
     2941 tgttcatcat ccagagcttt atcatgatga cccctagccc cacccgggac gccaagacct
     3001 tctgtagaaa ggccctcgtg aatggccagc tgatcgacag ctccgacctg gacgtggcca
     3061 gcgaggaaga ggacctgatc aactcctttg ccagcaagta caacctcatc tacgaaaaga
     3121 ttaagatgga agaactccgc gagaacgaac agaacaagaa ggccctgaaa accagcaaga
     3181 cccacctgtc cgctctggaa gtgcggaaca cccagaacat tgcccagggc atctctaccg
     3241 agaacaaact gaacagcgcc cggaacgccc cactgatcgc cgtggtcaca gatagcgcca
     3301 gcgaaaccac cgacgacatc attcagaaca acgtggacct cagcgccctg agccccgacg
     3361 tcgagcagac ctttcagaca ctgaatgcca agggcggatc tggcgcgccg tcgacctcca
     3421 tcacggccta taagagtgag ggggagtcag cggagttctc cttcccactc aaccttggag
     3481 aggaaagcct gcagggagag ttgagatgga aggcagagaa ggctccttct tcccagtcct
     3541 ggatcacctt ctccctaaag aaccaaaagg tgtctgtgca gaagtctact agcaacccca
     3601 agttccagct gtccgaaacg ctcccactca cccttcagat accccaggtc tcccttcagt
     3661 ttgctggttc tggcaacctg accctgactc tggacagagg gatactgtat caggaagtga
     3721 acctggtggt gatgaaagtg actcagcccg acagcaacac tttgacctgt gaggtgatgg
     3781 gacccacctc acccaagatg agactgatct tgaagcagga gaatcaggag gccagggtct
     3841 ccaggcagga gaaagtgatt caagtgcagg cccctgaagc aggggtgtgg caatgtctac
     3901 tgagtgaagg tgaagaggtc aagatggact ccaagatcca ggttttatcc aaagggttga
     3961 attccggcag cctgcaccac atcctggacg cccagaagat gctgtggaac cacagggaca
     4021 ggaacctgcc tcctctggcc cctctgggcc cccaccacca tcaccaccat tgagtctaga
     4081 cacgtgatta attaaggatc ccccgacctc gacctctggc taataaagga aatttatttt
     4141 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     4201 aaatcatttg gtcgagatcc ctcggagatc tctagctaga ggatcgatcc ccgccccgga
     4261 cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc
     4321 ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca cgggggggga
     4381 ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat gtgcacattt
     4441 gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac tgcaacacaa
     4501 cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac
     4561 ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg
     4621 tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt
     4681 gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac
     4741 cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta
     4801 aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt tttatttaca
     4861 tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag tggctgaaga
     4921 tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc tccttcgttt
     4981 agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca
     5041 aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg tggcattgtg
     5101 ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt gtaggaatga
     5161 aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc gtaaagactg
     5221 gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt gcaatatgat
     5281 actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca tgttgttaca
     5341 ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc cactggttgt
     5401 ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata aacaaagaca
     5461 agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc ccacacgccg
     5521 ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt aaccccgcta
     5581 accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg gaatacctgc
     5641 ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag gacaaattac
     5701 acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca cgaggtcgct
     5761 gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata tctggatagc
     5821 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
     5881 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc
     5941 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     6001 atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga ttagggtagt
     6061 atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg gatagcatat
     6121 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagcatag
     6181 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     6241 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     6301 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
     6361 gctatcctca tgataagctg tcaaacatga gaattttctt gaagacgaaa gggcctcgtg
     6421 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     6481 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     6541 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     6601 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     6661 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6721 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6781 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6841 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6901 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6961 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7021 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7081 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7141 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7201 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7261 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     7321 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     7381 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     7441 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     7501 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7561 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7621 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7681 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7741 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     7801 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7861 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7921 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7981 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8041 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8101 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8161 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8221 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8281 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     8341 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     8401 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     8461 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     8521 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     8581 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     8641 ctctagctag aggtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
     8701 cgttgttgcc attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc
     8761 aatattggca attagccata ttagtcattg gttatatagc ataaatcaat attggctatt
     8821 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
     8881 tatgaccgc
//
