LOCUS       sequence-242734-        7899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. ovale protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 7899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Po_MSP10-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3063..3080
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3132..3187
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3186..3205)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5423..5441)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5509..5613
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5614..5682,5683..6474)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5832..5851)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6645..7233
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7134..7153
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7387..7404
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7521..7542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7557..7562,7563..7580,7581..7587)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7595..7611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7600..7622
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7619..7635
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7619..7635
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcct
     1201 acatcaacga cgtgaaggac accaacgacg tcaagatcta caagcaggac aacgccaaga
     1261 acagcgggaa caacaacaac gaggccgtga ccgacgacgg cgaggaatgg gacaacgacg
     1321 acaaccacgc cgacgagaac gacgagacag agaacatcga cgacatggat aacaacaaga
     1381 acaacctgaa caccgccaac gtgatcaaga tgatgcagaa gtactacgtc cggaagcggc
     1441 ccgacaacgc ccacaagaac gacatgtaca ccgtggaacc cttccacacc gcctacctcc
     1501 agaagtacaa gaacctgaag gaccacagca atatcgccaa cgacaacaat aatgccgatg
     1561 agaacgtgga aaacgatgcc aatcacgcct tcgactatat cgtcgagaac atgatcccat
     1621 ttctgctgag caaagaggac aacaatcagg ccgccaccgg cgaggacgac aacagagaaa
     1681 tggatggcgg caagggcacc aagagcaacg agggccctgg caacaaccgg atcgacaagg
     1741 acccctccaa agagcagagc ggcgagaaga acgagcggag cgacgacagc agcagcaact
     1801 ctgcccacag cgagggcgac agccccaatg gcaagagcac cagcaacgag aagcggcctc
     1861 ccaacgagga aagcctgcac gagcagaccc ctagagagga aaccagccac gccgagacaa
     1921 cccctaccag cagcaagagc agcgaagagg acacccagaa gaaagaaagc cagaccccaa
     1981 tggtggaccc cagcgagcag atcaagaaaa ccctgctgaa agagggcaag gacatcaaag
     2041 agacaaccag cctgatcgac aatgccgtgt acaatgtgga acaggtcatc ctcaagaccc
     2101 ggttctacag caccgccatc cggaacttcg tgaacttcaa agtgaaccac atctgcgagt
     2161 acagcaagtg cggcctgaac gcccggtgct acatcatcga gaaggacaaa gaggaatgcc
     2221 gctgccgggc caactacgag caggatacca gcgtggaata cttcaagtgc aagcccatga
     2281 ccaagtggga ctgcaccatc aacaacggca actgcgacaa gaacgccgag tgcaccatcg
     2341 agaacgagag gatcaagtgc cagtgcggct tcaactactt cggcgacggc atcttctgcg
     2401 tgatgggctc tggcgcgccg tcgacctcca tcacggccta taagagtgag ggggagtcag
     2461 cggagttctc cttcccactc aaccttggag aggaaagcct gcagggagag ttgagatgga
     2521 aggcagagaa ggctccttct tcccagtcct ggatcacctt ctccctaaag aaccaaaagg
     2581 tgtctgtgca gaagtctact agcaacccca agttccagct gtccgaaacg ctcccactca
     2641 cccttcagat accccaggtc tcccttcagt ttgctggttc tggcaacctg accctgactc
     2701 tggacagagg gatactgtat caggaagtga acctggtggt gatgaaagtg actcagcccg
     2761 acagcaacac tttgacctgt gaggtgatgg gacccacctc acccaagatg agactgatct
     2821 tgaagcagga gaatcaggag gccagggtct ccaggcagga gaaagtgatt caagtgcagg
     2881 cccctgaagc aggggtgtgg caatgtctac tgagtgaagg tgaagaggtc aagatggact
     2941 ccaagatcca ggttttatcc aaagggttga attccggcag cctgcaccac atcctggacg
     3001 cccagaagat gctgtggaac cacagggaca ggaacctgcc tcctctggcc cctctgggcc
     3061 cccaccacca tcaccaccat tgagtctaga cacgtgatta attaaggatc ccccgacctc
     3121 gacctctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     3181 tctctcactc ggaaggacat atgggagggc aaatcatttg gtcgagatcc ctcggagatc
     3241 tctagctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga catctctgcc
     3301 ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg
     3361 gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt
     3421 tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc
     3481 agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa
     3541 gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg
     3601 ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg
     3661 cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc
     3721 cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg
     3781 gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc
     3841 accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac
     3901 cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat
     3961 cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac
     4021 agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat
     4081 ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc
     4141 cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat
     4201 ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat
     4261 gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag
     4321 ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg
     4381 acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct
     4441 ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga
     4501 gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt
     4561 gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa
     4621 accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc
     4681 gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg
     4741 gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg
     4801 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
     4861 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     4921 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     4981 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     5041 atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc
     5101 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat
     5161 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     5221 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
     5281 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     5341 gctatcctaa tctgtatccg ggtagcatat gctatcctca tgataagctg tcaaacatga
     5401 gaattttctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     5461 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5521 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5581 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5641 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5701 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5761 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5821 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     5881 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5941 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     6001 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     6061 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     6121 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
     6181 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6241 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6301 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6361 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6421 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6481 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6541 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6601 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6661 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6721 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6781 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6841 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6901 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6961 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7021 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7081 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7141 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7201 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7261 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7321 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7381 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     7441 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     7501 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     7561 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     7621 ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacca attctcatgt
     7681 ttgacagctt atcatcgcag atccgggcaa cgttgttgcc attgctgcag gcgcagaact
     7741 ggtaggtatg gaagatctat acattgaatc aatattggca attagccata ttagtcattg
     7801 gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata
     7861 atatgtacat ttatattggc tcatgtccaa tatgaccgc
//
