LOCUS       sequence-242740-        7524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. ovale protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7524)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 7524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Po_P12-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2688..2705
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2757..2812
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2811..2830)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5048..5066)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5134..5238
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5239..5307,5308..6099)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5457..5476)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6270..6858
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6759..6778
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7012..7029
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7146..7167
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7182..7187,7188..7205,7206..7212)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7220..7236
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7225..7247
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7244..7260
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7244..7260
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 agtgtgattt cacaagaggc agcctggacg tgtcccgcag cggcaacaac aaggtggccc
     1261 tgttcgagag agaggaagag gcctacgaca gcaacgtgaa gcactgcgtg aagttcacca
     1321 agggcctgga aatcatgacc ttcatctgcc ccaagcggag catccactac gaggccatcg
     1381 agatccggcc caccgactgc ttcgtgaaag tgcgcgtgaa cggcatcgag gaacggctga
     1441 acgatttcat gaagggcgtg atcttcgaga gcagagagaa cgacctgagc aacatccgga
     1501 aggtgttcat ccctcccacc atctacgagg atatcgtgtt cgagtgcacc tgtgacaaca
     1561 gcctgacctt cggcgacaac cagatcggca cccggggcat catgcgggtg cacctgaaga
     1621 aaaacctggt gttcggctgc gacttcgact acgacgtgat gaacagccgg aagcggagcg
     1681 ccttcgtgtc cttctacgag aagaacgaga tcaagcccgg cgaagagatc gtgtgcaacg
     1741 tcaagatcac caagaaagag gtgtacctgg gcctgatctg ccccgagggc tacaagatgt
     1801 accctctgac ctgcttcgag aacgtgctgc acgagaaaga agtcgtgaag atcaacgagc
     1861 tggtgcagca tgacgtgaag ctgcacatgg acgtgcacaa gcagatcagc ttcgccacct
     1921 tcaccctgaa caagaatgag aaccccaaca gcttcagctg ccactgtgtg aaggacggcg
     1981 accacgccag catcctgcag gccaacttcg cctacgccaa ctacgagagc gcctctggcg
     2041 cgccgtcgac ctccatcacg gcctataaga gtgaggggga gtcagcggag ttctccttcc
     2101 cactcaacct tggagaggaa agcctgcagg gagagttgag atggaaggca gagaaggctc
     2161 cttcttccca gtcctggatc accttctccc taaagaacca aaaggtgtct gtgcagaagt
     2221 ctactagcaa ccccaagttc cagctgtccg aaacgctccc actcaccctt cagatacccc
     2281 aggtctccct tcagtttgct ggttctggca acctgaccct gactctggac agagggatac
     2341 tgtatcagga agtgaacctg gtggtgatga aagtgactca gcccgacagc aacactttga
     2401 cctgtgaggt gatgggaccc acctcaccca agatgagact gatcttgaag caggagaatc
     2461 aggaggccag ggtctccagg caggagaaag tgattcaagt gcaggcccct gaagcagggg
     2521 tgtggcaatg tctactgagt gaaggtgaag aggtcaagat ggactccaag atccaggttt
     2581 tatccaaagg gttgaattcc ggcagcctgc accacatcct ggacgcccag aagatgctgt
     2641 ggaaccacag ggacaggaac ctgcctcctc tggcccctct gggcccccac caccatcacc
     2701 accattgagt ctagacacgt gattaattaa ggatcccccg acctcgacct ctggctaata
     2761 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     2821 gacatatggg agggcaaatc atttggtcga gatccctcgg agatctctag ctagaggatc
     2881 gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc ttcgcggggc
     2941 agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct gttccacatg
     3001 tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca tccttataaa
     3061 tggatgtgca catttgccaa cactgagtgg ctttcatcct ggagcagact ttgcagtctg
     3121 tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct tacaccaatg
     3181 ctgggggaca tgtacctccc aggggcccag gaagactacg ggaggctaca ccaacgtcaa
     3241 tcagaggggc ctgtgtagct accgataagc ggaccctcaa gagggcatta gcaatagtgt
     3301 ttataaggcc cccttgttaa ccctaaacgg gtagcatatg cttcccgggt agtagtatat
     3361 actatccaga ctaaccctaa ttcaatagca tatgttaccc aacgggaagc atatgctatc
     3421 gaattagggt tagtaaaagg gtcctaagga acagcgatat ctcccacccc atgagctgtc
     3481 acggttttat ttacatgggg tcaggattcc acgagggtag tgaaccattt tagtcacaag
     3541 ggcagtggct gaagatcaag gagcgggcag tgaactctcc tgaatcttcg cctgcttctt
     3601 cattctcctt cgtttagcta atagaataac tgctgagttg tgaacagtaa ggtgtatgtg
     3661 aggtgctcga aaacaaggtt tcaggtgacg cccccagaat aaaatttgga cggggggttc
     3721 agtggtggca ttgtgctatg acaccaatat aaccctcaca aaccccttgg gcaataaata
     3781 ctagtgtagg aatgaaacat tctgaatatc tttaacaata gaaatccatg gggtggggac
     3841 aagccgtaaa gactggatgt ccatctcaca cgaatttatg gctatgggca acacataatc
     3901 ctagtgcaat atgatactgg ggttattaag atgtgtccca ggcagggacc aagacaggtg
     3961 aaccatgttg ttacactcta tttgtaacaa ggggaaagag agtggacgcc gacagcagcg
     4021 gactccactg gttgtctcta acacccccga aaattaaacg gggctccacg ccaatggggc
     4081 ccataaacaa agacaagtgg ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc
     4141 agcccccaca cgccgccctg cggttttgga ctgtaaaata agggtgtaat aacttggctg
     4201 attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac taatggcacc
     4261 ccggggaata cctgcataag taggtgggcg ggccaagata ggggcgcgat tgctgcgatc
     4321 tggaggacaa attacacaca cttgcgcctg agcgccaagc acagggttgt tggtcctcat
     4381 attcacgagg tcgctgagag cacggtgggc taatgttgcc atgggtagca tatactaccc
     4441 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     4501 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     4561 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     4621 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctaat
     4681 agagattagg gtagtatatg ctatcctaat ttatatctgg gtagcatata ctacccaaat
     4741 atctggatag catatgctat cctaatctat atctgggtag catatgctat cctaatctat
     4801 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     4861 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     4921 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     4981 atccgggtag catatgctat cctcatgata agctgtcaaa catgagaatt ttcttgaaga
     5041 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     5101 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5161 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5221 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5281 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5341 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5401 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5461 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     5521 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5581 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5641 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5701 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5761 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     5821 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5881 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5941 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6001 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6061 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6121 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6181 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6241 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6301 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6361 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6421 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6481 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6541 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6601 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6661 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6721 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6781 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6841 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6901 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6961 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7021 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7081 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7141 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7201 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7261 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
     7321 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
     7381 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
     7441 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
     7501 ttggctcatg tccaatatga ccgc
//
