LOCUS       sequence-242743-        8058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. ovale protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 8058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Po_MSP3.5-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1596..1605
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3222..3239
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3291..3346
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3345..3364)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5582..5600)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5668..5772
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5773..5841,5842..6633)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5991..6010)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6804..7392
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7293..7312
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7546..7563
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7680..7701
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7716..7721,7722..7739,7740..7746)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7754..7770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7759..7781
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7778..7794
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7778..7794
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 aggacatcga ccacatcagc aacctggcca acaaagagaa gccccagctg cggaacggcc
     1261 tgagcagcaa gaatatcgcc acccagaacg gcaagtccaa cctggaaatc cggaacaact
     1321 acgacaacca cgccgacaag aacaacgaga tcagcggcaa catcgagatg gtggaaaaga
     1381 gcgccgagag cctggaacag ctggaaaagt tccaggtgga agcccaggac gacgctaaca
     1441 aagaggataa gaccaaagag atcgagcgcg ccaagaaggc caagaaccag gccgagatcc
     1501 tgctgaagaa agccgaggaa tacgtgaaga gagccggcaa ggccgtcgag agggccaaga
     1561 gcgtggaaag cgtgaacgag aaagccaagc tgagcgccgc catggtggaa gaggaagaga
     1621 acaacgccaa gaaagccctg gaagtggcca agaagctggt cgaggaaatc ggcatcgaga
     1681 tcgaggccac cagaaccgcc cctagcctgg acattgccaa gaaaaaggcc aacaacgccg
     1741 aaaccagcct gaagctggtg gaacagcagg cccagattgc cgaggtggcc gccgtgaatg
     1801 ccgaactggc cgtgtctcac gtggaacggg cctggattga ggccaagatc gccagctacc
     1861 tggaaaagat tgaggtgctg aatgagaggg ctaagaaggc ccagcagaaa accagagagg
     1921 ccaaagagcg ggccgataag gccgtgcagg ccgtgacaag cgtggaagcc atcaacgaga
     1981 acgctaagaa cgccgccacc cggaccagag agaaggccag agctgtcgag gcctccctga
     2041 agattgtgga agccgaagtg gctgtggccg agaaggcctc cattgaggcc gagaaagctg
     2101 ccaccctgaa gggcgagatc ggcaaagaac tggaacagac cgagatcacc accgaagaga
     2161 agcgggccaa agtggaagag acagtgaagg gcaagacaag agaggtcgaa gaggccaccg
     2221 ccaaggtcga ggccgccaca gaagaagccg aaaaggccgc caaagaggct gaggccgcct
     2281 ccaaagagac agagtccgcc agcaaagaag ctgagacacc ctccgaggaa gccattacca
     2341 ccagcgagca ggccagcacc gccgagtctc agaatagagc ccaggtcgga aacaacgctg
     2401 ccaaagaacg ggacgacacc gacgtgcaca aaggcaagga caaagagcag atcgacaagg
     2461 gcgaaatcaa agagaaaagc ctgcagcaga agaacggcga ggaagtcaag aacgaccagc
     2521 agcaggacaa gtccagcatc tacgagttca tcaccatgct gatcacccct ggcgcgccgt
     2581 cgacctccat cacggcctat aagagtgagg gggagtcagc ggagttctcc ttcccactca
     2641 accttggaga ggaaagcctg cagggagagt tgagatggaa ggcagagaag gctccttctt
     2701 cccagtcctg gatcaccttc tccctaaaga accaaaaggt gtctgtgcag aagtctacta
     2761 gcaaccccaa gttccagctg tccgaaacgc tcccactcac ccttcagata ccccaggtct
     2821 cccttcagtt tgctggttct ggcaacctga ccctgactct ggacagaggg atactgtatc
     2881 aggaagtgaa cctggtggtg atgaaagtga ctcagcccga cagcaacact ttgacctgtg
     2941 aggtgatggg acccacctca cccaagatga gactgatctt gaagcaggag aatcaggagg
     3001 ccagggtctc caggcaggag aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc
     3061 aatgtctact gagtgaaggt gaagaggtca agatggactc caagatccag gttttatcca
     3121 aagggttgaa ttccggcagc ctgcaccaca tcctggacgc ccagaagatg ctgtggaacc
     3181 acagggacag gaacctgcct cctctggccc ctctgggccc ccaccaccat caccaccatt
     3241 gagtctagac acgtgattaa ttaaggatcc cccgacctcg acctctggct aataaaggaa
     3301 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     3361 tgggagggca aatcatttgg tcgagatccc tcggagatct ctagctagag gatcgatccc
     3421 cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca
     3481 tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac
     3541 ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg
     3601 tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact
     3661 gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg
     3721 gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag
     3781 gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa
     3841 ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc
     3901 cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta
     3961 gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt
     4021 ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt
     4081 ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct
     4141 ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc
     4201 tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt
     4261 ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg
     4321 taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg
     4381 taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg
     4441 caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat
     4501 gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc
     4561 actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa
     4621 acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc
     4681 cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta
     4741 accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg
     4801 aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg
     4861 acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac
     4921 gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat
     4981 ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     5041 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
     5101 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     5161 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat
     5221 tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg
     5281 atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     5341 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     5401 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     5461 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     5521 gtagcatatg ctatcctcat gataagctgt caaacatgag aattttcttg aagacgaaag
     5581 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     5641 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5701 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5761 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5821 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5881 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5941 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6001 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6061 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6121 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6181 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6241 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6301 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6361 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6421 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6481 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6541 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6601 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6661 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6721 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6781 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6841 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6901 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6961 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7021 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7081 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7141 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7201 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7261 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7321 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7381 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7441 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7501 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7561 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7621 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     7681 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     7741 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     7801 tacgccaagc tctagctaga ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga
     7861 tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata
     7921 cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata
     7981 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
     8041 catgtccaat atgaccgc
//
