LOCUS       sequence-242745-        8814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. ovale protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8814)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 8814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Po_MSP3.6-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3978..3995
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4047..4102
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4101..4120)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6338..6356)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6424..6528
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6529..6597,6598..7389)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6747..6766)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7560..8148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8049..8068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8302..8319
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8436..8457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8472..8477,8478..8495,8496..8502)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8510..8526
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8515..8537
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8534..8550
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8534..8550
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 aggacgtgga caatatccac aacgccgcca acaacaacaa gggcaagccc aacctgcgga
     1261 acggcttcag cttcaagaac ttctttccac agaacgagca gaacgacctg gaagaggaag
     1321 atgacgacga cgagtacaac accgacgaga acaatgccga gatcattgcc aacggcgcca
     1381 aagagaaggc cgagaaggtc gaggaagcca agaacgccgg cgagaaggcc atcaagagag
     1441 ccaaagaggc cacctacagc gccatcgaga aagtgcggaa cgcccgggat gccattgaag
     1501 aggccaacaa ggccgtgaaa gagaaagaac agaacgccaa agaaaaagtg accaaggccg
     1561 aaaaggacac caacgacgcc atggaagatg ctaacaaagc caacaaagct gccgagatta
     1621 tcaagcagat caaggccgcc attgaggccg agagcgacga ggccaagtac aacgtggccc
     1681 tggaaaacat gctgaagaaa gtgaagtcca aggccaaaga agccgagatc gaggccgaaa
     1741 tcgaagctga gatccccaag gccctggccg ccaaaacaga ggccctgatc gccaagatcg
     1801 acgccgagaa agccaaaaac gaagccgtga atatcgccaa aaaggccaag gatgccgtgg
     1861 aaaaggccca ggtggccgct aagaaggcta aagaggccgc caataccgcc accgaacacg
     1921 ccctgcaggc tgagaaggct gaaaaagagg aaaagaccac cgagacactg gaaaaagtca
     1981 agaacattgc cgacgccgcc gtgggagccg ctggcgaggc taaaaaagct aagctgagcg
     2041 ccgagattgg cctgaagctg accaaagccc aggaagctaa agaagaggcc gaagaagcag
     2101 aagaggctgt cgaggctgtg aacgccgcaa aagaagaagt gaacaaagca gagcagacca
     2161 ccgccgaaga gacagccaac gaggtggcca agagcgtggt caagatcgcc cagaccaaag
     2221 tgacaaaagc cattgagaac gccgctgcca aggcaaagga cgccgcctct gccgcagaga
     2281 aggcaaagac catcgaaacc ctgcccgaag tgaccgccga ggcccagaca gctaagaatg
     2341 ccgcccacga ggcaaacaaa gtgaaagagc gggctgagat cgccgccaag attgcaaaag
     2401 aggtggcaaa ggccgaagtg gcccggaaga aggccaagct ggctaaggaa gccgccgaca
     2461 aggctaagga tgaggtggaa cggatcgcca gagcccctaa ctccgccgag aagattaagc
     2521 aggccgctaa cgaggctctg caggccgcaa agagcgccga aacaaaagaa aaggaagtgg
     2581 aagtcaaggc tatcgacgcc ttcgaagagg caaagaaaga gaagtataag accgaggccg
     2641 aggtcgccac agaagtggcc aaaatcgagg gcgccaagac cgagacagag agcctgctga
     2701 tcaaggctca ggccgataag gccgtggcca ccgccacagc cgtcgagaag gtggaaacag
     2761 ccaccgccgg caccaagaac tacaaagaaa aggccagcct ggtggacgcc gccctgaatt
     2821 ctgccaatgc cgccgtgaca caggaagaag aagccgtcaa gcaggccctg gatatcgcta
     2881 tcgccgacaa ccccaacatg gtggaactcg agtacaccag cctgggcgag aacgaggaca
     2941 ccacctacca cgtggaagat gtgtccgagg aacaggaaga gggcagcgag gacgagagca
     3001 aggacaaaca gggcgtggtg cagaagtacg aggaagagga ggaaggcgag gaagaagagg
     3061 tcgaggaaga ggacgaggaa aacgaagagg acgagggcga cgaagagaaa gtgaaagaag
     3121 tggtcgagaa agtcaacgtg gcaaagggcg agtccaagga ccagaaagaa aacaagaacg
     3181 atggcgcccc tctgaacgac gacaacgcct acaccttcct ggaaggcaac tacaagaatg
     3241 acgactccac caagaaagcc gacggcgccc tgttcgagac aatcagcgac ctggtcatga
     3301 tcacccagca gctcctcatc ccttctggcg cgccgtcgac ctccatcacg gcctataaga
     3361 gtgaggggga gtcagcggag ttctccttcc cactcaacct tggagaggaa agcctgcagg
     3421 gagagttgag atggaaggca gagaaggctc cttcttccca gtcctggatc accttctccc
     3481 taaagaacca aaaggtgtct gtgcagaagt ctactagcaa ccccaagttc cagctgtccg
     3541 aaacgctccc actcaccctt cagatacccc aggtctccct tcagtttgct ggttctggca
     3601 acctgaccct gactctggac agagggatac tgtatcagga agtgaacctg gtggtgatga
     3661 aagtgactca gcccgacagc aacactttga cctgtgaggt gatgggaccc acctcaccca
     3721 agatgagact gatcttgaag caggagaatc aggaggccag ggtctccagg caggagaaag
     3781 tgattcaagt gcaggcccct gaagcagggg tgtggcaatg tctactgagt gaaggtgaag
     3841 aggtcaagat ggactccaag atccaggttt tatccaaagg gttgaattcc ggcagcctgc
     3901 accacatcct ggacgcccag aagatgctgt ggaaccacag ggacaggaac ctgcctcctc
     3961 tggcccctct gggcccccac caccatcacc accattgagt ctagacacgt gattaattaa
     4021 ggatcccccg acctcgacct ctggctaata aaggaaattt attttcattg caatagtgtg
     4081 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttggtcga
     4141 gatccctcgg agatctctag ctagaggatc gatccccgcc ccggacgaac taaacctgac
     4201 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     4261 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     4321 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     4381 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     4441 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     4501 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     4561 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     4621 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     4681 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     4741 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     4801 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     4861 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
     4921 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
     4981 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
     5041 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
     5101 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
     5161 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
     5221 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
     5281 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
     5341 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
     5401 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
     5461 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
     5521 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
     5581 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
     5641 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
     5701 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     5761 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     5821 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     5881 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     5941 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
     6001 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     6061 atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     6121 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     6181 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     6241 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgata
     6301 agctgtcaaa catgagaatt ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     6361 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     6421 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     6481 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     6541 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     6601 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     6661 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     6721 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     6781 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     6841 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     6901 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     6961 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7021 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     7081 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7141 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7201 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7261 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7321 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7381 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     7441 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7501 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7561 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7621 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7681 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     7741 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7801 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7861 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7921 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7981 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     8041 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     8101 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8161 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     8221 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     8281 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     8341 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     8401 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     8461 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     8521 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc
     8581 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
     8641 tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag
     8701 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     8761 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgc
//
