LOCUS       sequence-242746-        7944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. ovale protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 7944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Po_MSP7.12-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3108..3125
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3177..3232
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3231..3250)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5468..5486)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5554..5658
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5659..5727,5728..6519)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5877..5896)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6690..7278
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7179..7198
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7432..7449
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7566..7587
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7602..7607,7608..7625,7626..7632)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7640..7656
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7645..7667
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7664..7680
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7664..7680
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 agagaaagat cacaacagtg ggccagggcg gcgactacaa cgagaacgtg gtcaacatcc
     1261 tgagccagaa actgggcaac gtgtacaacg ccgaccccag caacgccaac gaggccgtga
     1321 acaagaagta caagctgatc aagaaagaga tggaagaact gcagcagtac gagaaagaga
     1381 acgccgaaga ggacttccag cagaacttca tgaccgagac agacgagaca agcggcaaga
     1441 agaaaaccat ctttggcgtg gacgaggacg acctggacag ctacgacgag gaattcttcg
     1501 gccagagcaa gaagttcatc aagggcgagg ccagcggcga cgacgtgcac agcggaaata
     1561 gcctggaagc cgaggaaccc aaagaggtgg ccagcagccc tcctcatggc gatctggccg
     1621 gaacaggcct gacacctggc agacagagcg agatcgagca gctgaagggc gagagagtgg
     1681 aaaccggccc tgacgtgaag atcagcagca cccatgtgcg gagcgacggc tctcctgaga
     1741 gcgcccctaa agagagcctg cagcagccca acaccgccga cctggaagca gaaagacggg
     1801 ctggcgtgac caccccttct gacggccagc agagaacagt ggctgccggc cctacaccta
     1861 gcagcccacc tcccaatccc caggacccta gcaagaccag cgaccccaag ggcagcgaga
     1921 gccagagaaa cgtgcaggaa accagcgagg tccagagaga gaacctggaa agcagcaccg
     1981 gcaacctgga acctggcgtg ccatctgccg tggccgagga tacaaagcag cctcccgagc
     2041 agatcaccga ggccacagcc cctgtggtca agtacatgga cagcctgtac gacgagatca
     2101 tcaccaagag cggccagaac gagaaggccg agaacaccaa gaagcacagc aactacgcca
     2161 acctgaagaa aaagtacaac ttccccatga acggcaaaga gtacaacatg gtcaagcagc
     2221 tgttcggcga gtgctttaag aaagaggaca ccgccaacgt cgccgagaat gccgcctgtc
     2281 aggtggacgt gctggccaac gtgctgcagg acaaagagtt cagcgagcag ttcgacaact
     2341 tcatgcacgg cctgtacagc ttcgccaagc ggcacaccta cctgagcgac gccagactga
     2401 aggacgagaa cgccatcaac ggctttctga agaacgccct gagcctgctg aacacaggcg
     2461 cgccgtcgac ctccatcacg gcctataaga gtgaggggga gtcagcggag ttctccttcc
     2521 cactcaacct tggagaggaa agcctgcagg gagagttgag atggaaggca gagaaggctc
     2581 cttcttccca gtcctggatc accttctccc taaagaacca aaaggtgtct gtgcagaagt
     2641 ctactagcaa ccccaagttc cagctgtccg aaacgctccc actcaccctt cagatacccc
     2701 aggtctccct tcagtttgct ggttctggca acctgaccct gactctggac agagggatac
     2761 tgtatcagga agtgaacctg gtggtgatga aagtgactca gcccgacagc aacactttga
     2821 cctgtgaggt gatgggaccc acctcaccca agatgagact gatcttgaag caggagaatc
     2881 aggaggccag ggtctccagg caggagaaag tgattcaagt gcaggcccct gaagcagggg
     2941 tgtggcaatg tctactgagt gaaggtgaag aggtcaagat ggactccaag atccaggttt
     3001 tatccaaagg gttgaattcc ggcagcctgc accacatcct ggacgcccag aagatgctgt
     3061 ggaaccacag ggacaggaac ctgcctcctc tggcccctct gggcccccac caccatcacc
     3121 accattgagt ctagacacgt gattaattaa ggatcccccg acctcgacct ctggctaata
     3181 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     3241 gacatatggg agggcaaatc atttggtcga gatccctcgg agatctctag ctagaggatc
     3301 gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc ttcgcggggc
     3361 agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct gttccacatg
     3421 tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca tccttataaa
     3481 tggatgtgca catttgccaa cactgagtgg ctttcatcct ggagcagact ttgcagtctg
     3541 tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct tacaccaatg
     3601 ctgggggaca tgtacctccc aggggcccag gaagactacg ggaggctaca ccaacgtcaa
     3661 tcagaggggc ctgtgtagct accgataagc ggaccctcaa gagggcatta gcaatagtgt
     3721 ttataaggcc cccttgttaa ccctaaacgg gtagcatatg cttcccgggt agtagtatat
     3781 actatccaga ctaaccctaa ttcaatagca tatgttaccc aacgggaagc atatgctatc
     3841 gaattagggt tagtaaaagg gtcctaagga acagcgatat ctcccacccc atgagctgtc
     3901 acggttttat ttacatgggg tcaggattcc acgagggtag tgaaccattt tagtcacaag
     3961 ggcagtggct gaagatcaag gagcgggcag tgaactctcc tgaatcttcg cctgcttctt
     4021 cattctcctt cgtttagcta atagaataac tgctgagttg tgaacagtaa ggtgtatgtg
     4081 aggtgctcga aaacaaggtt tcaggtgacg cccccagaat aaaatttgga cggggggttc
     4141 agtggtggca ttgtgctatg acaccaatat aaccctcaca aaccccttgg gcaataaata
     4201 ctagtgtagg aatgaaacat tctgaatatc tttaacaata gaaatccatg gggtggggac
     4261 aagccgtaaa gactggatgt ccatctcaca cgaatttatg gctatgggca acacataatc
     4321 ctagtgcaat atgatactgg ggttattaag atgtgtccca ggcagggacc aagacaggtg
     4381 aaccatgttg ttacactcta tttgtaacaa ggggaaagag agtggacgcc gacagcagcg
     4441 gactccactg gttgtctcta acacccccga aaattaaacg gggctccacg ccaatggggc
     4501 ccataaacaa agacaagtgg ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc
     4561 agcccccaca cgccgccctg cggttttgga ctgtaaaata agggtgtaat aacttggctg
     4621 attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac taatggcacc
     4681 ccggggaata cctgcataag taggtgggcg ggccaagata ggggcgcgat tgctgcgatc
     4741 tggaggacaa attacacaca cttgcgcctg agcgccaagc acagggttgt tggtcctcat
     4801 attcacgagg tcgctgagag cacggtgggc taatgttgcc atgggtagca tatactaccc
     4861 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     4921 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     4981 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     5041 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctaat
     5101 agagattagg gtagtatatg ctatcctaat ttatatctgg gtagcatata ctacccaaat
     5161 atctggatag catatgctat cctaatctat atctgggtag catatgctat cctaatctat
     5221 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     5281 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     5341 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     5401 atccgggtag catatgctat cctcatgata agctgtcaaa catgagaatt ttcttgaaga
     5461 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     5521 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5581 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5641 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5701 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5761 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5821 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5881 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     5941 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6001 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6061 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6121 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6181 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     6241 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6301 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6361 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6421 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6481 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6541 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6601 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6661 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6721 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6781 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6841 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6901 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6961 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7021 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7081 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7141 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7201 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7261 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7321 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7381 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7441 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7501 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7561 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7621 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7681 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
     7741 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
     7801 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
     7861 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
     7921 ttggctcatg tccaatatga ccgc
//
