LOCUS       sequence-242747-        7758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. ovale protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 7758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Po_MSP7.8-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2922..2939
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2991..3046
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3045..3064)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5282..5300)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5368..5472
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5473..5541,5542..6333)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5691..5710)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6504..7092
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6993..7012
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7246..7263
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7380..7401
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7416..7421,7422..7439,7440..7446)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7454..7470
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7459..7481
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7478..7494
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7478..7494
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 aagtgaccgc caagaacaag cagaagaact acgccaaaga gggcttcctg aacctgctga
     1261 acaagaagct ggaaaacctg aacaacgccg tgtccggcga ggtgtccgcc aacttcaaca
     1321 aggacatcga gctgctgaag aaagagatcg aggaactgca gaagtacgag aaggcctacg
     1381 gcgccgagga tttcgacgag aacctggaag atgagagcgg cgacggcctg ggcaagaaga
     1441 aaaagatcct gggcgtggac gaggacgagc tggacaacta cgacgaggaa ttcttcggcc
     1501 agagcaagac caccatgaag ggcgaggact accaggaaga taccgacgct caggacgaca
     1561 ccgacgagca gggcgagaca gatgcccagg atgacagcag cggcgaggat caccacatgg
     1621 tgcctggcga gatcgccgag ggcaatagcc ctctggaagg caccaacgac aagggcgctg
     1681 cccagaagtc tggcgagttc ccacccaaag agacaagcaa cggcaaggtg tccctggatg
     1741 gcgccgaccc tcacaacgag gccagcaatg gacatgatgc cccacctggc gaggtgccac
     1801 cttacggcgt ggaaagcacc gatcctgagg tggcccctgg aaagcagaag gcccctgagc
     1861 agggcttcaa caccgccaac gccgaggtgc agtacctgga caacctgtac gacgacgcca
     1921 tcagagagac aaacaagaac aaccaggtgc acaaccctca gcaccacacc aactacgata
     1981 ccctgaacaa aaactacgac ttccccatga ccagcaaaga atacgacatg gtcaagatgc
     2041 tgttcggcga ctgcttcaag atgggcaacg acgagaatag caacaccgtg tgcctgatcg
     2101 acctgttcaa gaagattctc gaggacaaga tctttatgaa gcagttcgag aacttcatgc
     2161 acagcatcta cagcttcgcc aagcggcaca actacctggg ccacgagaag atgaagaacg
     2221 aggaactcta caaagagctg ttcaacagcg ccctgaagct gctcaacaca ggcgcgccgt
     2281 cgacctccat cacggcctat aagagtgagg gggagtcagc ggagttctcc ttcccactca
     2341 accttggaga ggaaagcctg cagggagagt tgagatggaa ggcagagaag gctccttctt
     2401 cccagtcctg gatcaccttc tccctaaaga accaaaaggt gtctgtgcag aagtctacta
     2461 gcaaccccaa gttccagctg tccgaaacgc tcccactcac ccttcagata ccccaggtct
     2521 cccttcagtt tgctggttct ggcaacctga ccctgactct ggacagaggg atactgtatc
     2581 aggaagtgaa cctggtggtg atgaaagtga ctcagcccga cagcaacact ttgacctgtg
     2641 aggtgatggg acccacctca cccaagatga gactgatctt gaagcaggag aatcaggagg
     2701 ccagggtctc caggcaggag aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc
     2761 aatgtctact gagtgaaggt gaagaggtca agatggactc caagatccag gttttatcca
     2821 aagggttgaa ttccggcagc ctgcaccaca tcctggacgc ccagaagatg ctgtggaacc
     2881 acagggacag gaacctgcct cctctggccc ctctgggccc ccaccaccat caccaccatt
     2941 gagtctagac acgtgattaa ttaaggatcc cccgacctcg acctctggct aataaaggaa
     3001 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     3061 tgggagggca aatcatttgg tcgagatccc tcggagatct ctagctagag gatcgatccc
     3121 cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca
     3181 tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac
     3241 ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg
     3301 tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact
     3361 gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg
     3421 gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag
     3481 gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa
     3541 ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc
     3601 cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta
     3661 gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt
     3721 ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt
     3781 ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct
     3841 ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc
     3901 tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt
     3961 ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg
     4021 taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg
     4081 taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg
     4141 caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat
     4201 gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc
     4261 actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa
     4321 acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc
     4381 cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta
     4441 accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg
     4501 aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg
     4561 acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac
     4621 gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat
     4681 ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     4741 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
     4801 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     4861 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat
     4921 tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg
     4981 atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     5041 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     5101 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     5161 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     5221 gtagcatatg ctatcctcat gataagctgt caaacatgag aattttcttg aagacgaaag
     5281 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     5341 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5401 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5461 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5521 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5581 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5641 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5701 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5761 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5821 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5881 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5941 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6001 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6061 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6121 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6181 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6241 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6301 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6361 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6421 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6481 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6541 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6601 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6661 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6721 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6781 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6841 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6901 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6961 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7021 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7081 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7141 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7201 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7261 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7321 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     7381 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     7441 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     7501 tacgccaagc tctagctaga ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga
     7561 tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata
     7621 cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata
     7681 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
     7741 catgtccaat atgaccgc
//
