LOCUS       sequence-242762-       10538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10538)
  TITLE     Howley lab plasmids
REFERENCE   2  (bases 1 to 10538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p1203 PML2d HPV-16
FEATURES             Location/Qualifiers
     source          1..10538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(7988..8007)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      complement(8694..9282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8774..8793)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9453..10244,10245..10313))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10076..10095
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10314..10418)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10486..10504
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttatcatcga taagctccgg atccccatgt accaatgttg cagtaaatcc aggtgattgt
       61 ccaccattag agttaataaa cacagttatt caggatggtg atatggttca tactggcttt
      121 ggtgctatgg actttactac attacaggct aacaaaagtg aagttccact ggatatttgt
      181 acatctattt gcaaatatcc agattatatt aaaatggtgt cagaaccata tggcgacagc
      241 ttattttttt atttacgaag ggaacaaatg tttgttagac atttatttaa tagggctggt
      301 actgttggtg aaaatgtacc agacgattta tacattaaag gctctgggtc tactgcaaat
      361 ttagccagtt caaattattt tcctacacct agtggttcta tggttacctc tgatgcccaa
      421 atattcaata aaccttattg gttacaacga gcacagggcc acaataatgg catttgttgg
      481 ggtaaccaac tatttgttac tgttgttgat actacacgca gtacaaatat gtcattatgt
      541 gctgccatat ctacttcaga aactacatat aaaaatacta actttaagga gtacctacga
      601 catggggagg aatatgattt acagtttatt tttcaactgt gcaaaataac cttaactgca
      661 gacgttatga catacataca ttctatgaat tccactattt tggaggactg gaattttggt
      721 ctacaacctc ccccaggagg cacactagaa gatacttata ggtttgtaac atcccaggca
      781 attgcttgtc aaaaacatac acctccagca cctaaagaag atccccttaa aaaatacact
      841 ttttgggaag taaatttaaa ggaaaagttt tctgcagacc tagatcagtt tcctttagga
      901 cgcaaatttt tactacaagc aggattgaag gccaaaccaa aatttacatt aggaaaacga
      961 aaagctacac ccaccacctc atctacctct acaactgcta aacgcaaaaa acgtaagctg
     1021 taagtattgt atgtatgttg aattagtgtt gtttgttgtg tatatgtttg tatgtgcttg
     1081 tatgtgcttg taaatattaa gttgtatgtg tgtttgtatg tatggtataa taaacacgtg
     1141 tgtatgtgtt tttaaatgct tgtgtaacta ttgtgtcatg caacataaat aaacttattg
     1201 tttcaacacc tactaattgt gttgtggtta ttcattgtat ataaactata tttgctacat
     1261 cctgtttttg ttttatatat actatatttt gtagcgccag cggccatttt gtagcttcaa
     1321 ccgaattcgg ttgcatgctt tttggcacaa aatgtgtttt tttaaatagt tctatgtcag
     1381 caactatggt ttaaacttgt acgtttcctg cttgccatgc gtgccaaatc cctgttttcc
     1441 tgacctgcac tgcttgccaa ccattccatt gttttttaca ctgcactatg tgcaactact
     1501 gaatcactat gtacattgtg tcatataaaa taaatcacta tgcgccaacg ccttacatac
     1561 cgctgttagg cacatatttt tggcttgttt taactaacct aattgcatat ttggcataag
     1621 gtttaaactt ctaaggccaa ctaaatgtca ccctagttca tacatgaact gtgtaaaggt
     1681 tagtcataca ttgttcattt gtaaaactgc acatgggtgt gtgcaaaccg ttttgggtta
     1741 cacatttaca agcaacttat ataataatac taaactacaa taattcatgt ataaaactaa
     1801 gggcgtaacc gaaatcggtt gaaccgaaac cggttagtat aaaagcagac attttatgca
     1861 ccaaaagaga actgcaatgt ttcaggaccc acaggagcga cccagaaagt taccacagtt
     1921 atgcacagag ctgcaaacaa ctatacatga tataatatta gaatgtgtgt actgcaagca
     1981 acagttactg cgacgtgagg tatatgactt tgcttttcgg gatttatgca tagtatatag
     2041 agatgggaat ccatatgctg tatgtgataa atgtttaaag ttttattcta aaattagtga
     2101 gtatagacat tattgttata gtttgtatgg aacaacatta gaacagcaat acaacaaacc
     2161 gttgtgtgat ttgttaatta ggtgtattaa ctgtcaaaag ccactgtgtc ctgaagaaaa
     2221 gcaaagacat ctggacaaaa agcaaagatt ccataatata aggggtcggt ggaccggtcg
     2281 atgtatgtct tgttgcagat catcaagaac acgtagagaa acccagctgt aatcatgcat
     2341 ggagatacac ctacattgca tgaatatatg ttagatttgc aaccagagac aactgatctc
     2401 tactgttatg agcaattaaa tgacagctca gaggaggagg atgaaataga tggtccagct
     2461 ggacaagcag aaccggacag agcccattac aatattgtaa ccttttgttg caagtgtgac
     2521 tctacgcttc ggttgtgcgt acaaagcaca cacgtagaca ttcgtacttt ggaagacctg
     2581 ttaatgggca cactaggaat tgtgtgcccc atctgttctc agaaaccata atctaccatg
     2641 gctgatcctg caggtaccaa tggggaagag ggtacgggat gtaatggatg gttttatgta
     2701 gaggctgtag tggaaaaaaa aacaggggat gctatatcag atgacgagaa cgaaaatgac
     2761 agtgatacag gtgaagattt ggtagatttt atagtaaatg ataatgatta tttaacacag
     2821 gcagaaacag agacagcaca tgcgttgttt actgcacagg aagcaaaaca acatagagat
     2881 gcagtacagg ttctaaaacg aaagtatttg ggtagtccac ttagtgatat tagtggatgt
     2941 gtagacaata atattagtcc tagattgaaa gctatatgta tagaaaaaca aagtagagct
     3001 gcaaaaagga gattatttga aagcgaagac agcgggtatg gcaatactga agtggaaact
     3061 cagcagatgt tacaggtaga agggcgccat gagactgaaa caccatgtag tcagtatagt
     3121 ggtggaagtg ggggtggttg cagtcagtac agtagtggaa gtgggggaga gggtgttagt
     3181 gaaagacaca ctatatgcca aacaccactt acaaatattt taaatgtact aaaaactagt
     3241 aatgcaaagg cagcaatgtt agcaaaattt aaagagttat acggggtgag tttttcagaa
     3301 ttagtaagac catttaaaag taataaatca acgtgttgcg attggtgtat tgctgcattt
     3361 ggacttacac ccagtatagc tgacagtata aaaacactat tacaacaata ttgtttatat
     3421 ttacacattc aaagtttagc atgttcatgg ggaatggttg tgttactatt agtaagatat
     3481 aaatgtggaa aaaatagaga aacaattgaa aaattgctgt ctaaactatt atgtgtgtct
     3541 ccaatgtgta tgatgataga gcctccaaaa ttgcgtagta cagcagcagc attatattgg
     3601 tataaaacag gtatgtcaaa tattagtgaa gtgtatggag acacgccaga atggatacaa
     3661 agacaaacag tattacaaca tagttttaat gattgtacat ttgaattatc acagatggta
     3721 caatgggcct acgataatga catagtagac gatagtgaaa ttgcatataa atatgcacaa
     3781 ttggcagaca ctaatagtaa tgcaagtgcc tttctaaaaa gtaattcaca ggcaaaaatt
     3841 gtaaaggatt gtgcaacaat gtgtagacat tataaacgag cagaaaaaaa acaaatgagt
     3901 atgagtcaat ggataaaata tagatgtgat agggtagatg atggaggtga ttggaagcaa
     3961 attgttatgt ttttaaggta tcaaggtgta gagtttatgt catttttaac tgcattaaaa
     4021 agatttttgc aaggcatacc taaaaaaaat tgcatattac tatatggtgc agctaacaca
     4081 ggtaaatcat tatttggtat gagtttaatg aaatttctgc aagggtctgt aatatgtttt
     4141 gtaaattcta aaagccattt ttggttacaa ccattagcag atgccaaaat aggtatgtta
     4201 gatgatgcta cagtgccctg ttggaactac atagatgaca atttaagaaa tgcattggat
     4261 ggaaatttag tttctatgga tgtaaagcat agaccattgg tacaactaaa atgccctcca
     4321 ttattaatta catctaacat taatgctggt acagattcta ggtggcctta tttacataat
     4381 agattggtgg tgtttacatt tcctaatgag tttccatttg acgaaaacgg aaatccagtg
     4441 tatgagctta atgataagaa ctggaaatcc tttttctcaa ggacgtggtc cagattaagt
     4501 ttgcacgagg acgaggacaa ggaaaacgat ggagactctt tgccaacgtt taaatgtgtg
     4561 tcaggacaaa atactaacac attatgaaaa tgatagtaca gacctacgtg accatataga
     4621 ctattggaaa cacatgcgcc tagaatgtgc tatttattac aaggccagag aaatgggatt
     4681 taaacatatt aaccaccagg tggtgccaac actggctgta tcaaagaata aagcattaca
     4741 agcaattgaa ctgcaactaa cgttagaaac aatatataac tcacaatata gtaatgaaaa
     4801 gtggacatta caagacgtta gccttgaagt gtatttaact gcaccaacag gatgtataaa
     4861 aaaacatgga tatacagtgg aagtgcagtt tgatggagac atatgcaata caatgcatta
     4921 tacaaactgg acacatatat atatttgtga agaagcatca gtaactgtgg tagagggtca
     4981 agttgactat tatggtttat attatgttca tgaaggaata cgaacatatt ttgtgcagtt
     5041 taaagatgat gcagaaaaat atagtaaaaa taaagtatgg gaagttcatg cgggtggtca
     5101 ggtaatatta tgtcctacat ctgtgtttag cagcaacgaa gtatcctctc ctgaaattat
     5161 taggcagcac ttggccaacc accccgccgc gacccatacc aaagccgtcg ccttgggcac
     5221 cgaagaaaca cagacgacta tccagcgacc aagatcagag ccagacaccg gaaacccctg
     5281 ccacaccact aagttgttgc acagagactc agtggacagt gctccaatcc tcactgcatt
     5341 taacagctca cacaaaggac ggattaactg taatagtaac actacaccca tagtacattt
     5401 aaaaggtgat gctaatactt taaaatgttt aagatataga tttaaaaagc attgtacatt
     5461 gtatactgca gtgtcgtcta catggcattg gacaggacat aatgtaaaac ataaaagtgc
     5521 aattgttaca cttacatatg atagtgaatg gcaacgtgac caatttttgt ctcaagttaa
     5581 aataccaaaa actattacag tgtctactgg atttatgtct atatgacaaa tcttgatact
     5641 gcatccacaa cattactggc gtgctttttg ctttgctttt gtgtgctttt gtgtgtctgc
     5701 ctattaatac gtccgctgct tttgtctgtg tctacataca catcattaat aatattggta
     5761 ttactattgt ggataacagc agcctctgcg tttaggtgtt ttattgtata tattatattt
     5821 gtttatatac cattattttt aatacataca catgcacgct ttttaattac ataatgtata
     5881 tgtacataat gtaattgtta catataattg ttgtatacca taacttacta ttttttcttt
     5941 tttattttca tatataattt ttttttttgt ttgtttgttt gttttttaat aaactgttat
     6001 tacttaacaa tgcgacacaa acgttctgca aaacgcacaa aacgtgcatc ggctacccaa
     6061 ctttataaaa catgcaaaca ggcaggtaca tgtccacctg acattatacc taaggttgaa
     6121 ggcaaaacta ttgctgatca aatattacaa tatggaagta tgggtgtatt ttttggtggg
     6181 ttaggaattg gaacagggtc gggtacaggc ggacgcactg ggtatattcc attgggaaca
     6241 aggcctccca cagctacaga tacacttgct cctgtaagac cccctttaac agtagatcct
     6301 gtgggccctt ctgatccttc tatagtttct ttagtggaag aaactagttt tattgatgct
     6361 ggtgcaccaa catctgtacc ttccattccc ccagatgtat caggatttag tattactact
     6421 tcaactgata ccacacctgc tatattagat attaataata ctgttactac tgttactaca
     6481 cataataatc ccactttcac tgacccatct gtattgcagc ctccaacacc tgcagaaact
     6541 ggagggcatt ttacactttc atcatccact attagtacac ataattatga agaaattcct
     6601 atggatacat ttattgttag cacaaaccct aacacagtaa ctagtagcac acccatacca
     6661 gggtctcgcc cagtggcacg cctaggatta tatagtcgca caacacaaca ggttaaagtt
     6721 gtagaccctg cttttgtaac cactcccact aaacttatta catatgataa tcctgcatat
     6781 gaaggtatag atgtggataa tacattatat ttttctagta atgataatag tattaatata
     6841 gctccagatc ctgacttttt ggatatagtt gctttacata ggccagcatt aacctctagg
     6901 cgtactggca ttaggtacag tagaattggt aataaacaaa cactacgtac tcgtagtgga
     6961 aaatctatag gtgctaaggt acattattat tatgatttaa gtactattga tcctgcagaa
     7021 gaaatagaat tacaaactat aacaccttct acatatacta ccacttcaca tgcagcctca
     7081 cctacttcta ttaataatgg attatatgat atttatgcag atgactttat tacagatact
     7141 tctacaaccc cggtaccatc tgtaccctct acatctttat caggttatat tcctgcaaat
     7201 acaacaattc cttttggtgg tgcatacaat attcctttag tatcaggtcc tgatataccc
     7261 attaatataa ctgaccaagc tccttcatta attcctatag ttccagggtc tccacaatat
     7321 acaattattg ctgatgcagg tgacttttat ttacatccta gttattacat gttacgaaaa
     7381 cgacgtaaac gtttaccata ttttttttca gatgtctctt tggctgccta gtgaggccac
     7441 tgtctacttg cctcctgtcc cagtatctaa ggttgtaagc acggatgaat atgttgcacg
     7501 cacaaacata tattatcatg caggaacatc cagactactt gcagttggac atccctattt
     7561 tcctattaaa aaacctaaca ataacaaaat attagttcct aaagtatcag gattacaata
     7621 cagggtattt agaatacatt tacctgaccc caataagttt ggttttcctg acacctcatt
     7681 ttataatcca gatacacagc ggctggtttg ggcctgtgta ggtgttgagg taggtcgtgg
     7741 tcagccatta ggtgtgggca ttagtggcca tcctttatta aataaattgg atgacacaga
     7801 aaatgctagt gcttatgcag caaatgcagg tgtggataat agagaatgta tatctatgga
     7861 ttacaaacaa acacaattgt gtttaattgg ttgcaaacca cctatagggg aacactgggg
     7921 caaaggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     7981 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     8041 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     8101 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     8161 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga
     8221 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     8281 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
     8341 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
     8401 ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
     8461 gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg
     8521 ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc
     8581 gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag
     8641 cttcaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8701 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8761 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8821 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8881 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8941 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9001 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9061 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9121 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9181 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9241 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9301 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9361 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9421 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9481 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9541 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9601 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9661 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9721 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
     9781 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9841 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9901 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9961 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10021 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
    10081 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10141 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10201 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10261 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10321 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10381 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10441 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
    10501 aggccctttc gtcttcaaga attctcatgt ttgacagc
//
