LOCUS       sequence-242817-        3596 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  complementation Vector for Phaeodactylum tricornutum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Madhuri S, Rio Bartulos C, Serif M, Lepetit B, Kroth PG.
  TITLE     A strategy to complement PtAUREO1a in TALEN knockout strains of 
            Phaeodactylum tricornutum.
  JOURNAL   Algal Research 2019 May;39:101469
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Algal 
            Research"; date: "2019-05"; volume: "39"; pages: "10146"
COMMENT     Sequence Label: pM9-4Compln
FEATURES             Location/Qualifiers
     source          1..3596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        44..62
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     44..61
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1293..1312)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1294..1312)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1399..1416)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1570..2158)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1650..1669)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2329..3120,3121..3189))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2952..2971
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3190..3294)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3362..3380
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3418..3440)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3540..3559
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg tgacactata
       61 gaaccagatc ccccaagctt gtttaaacga acagttggga gtctctatcc ttccttaaaa
      121 atttaatttt cattagttgc agtcactccg ctttggtttc acagtcagga ataacactag
      181 ctcgtcttca ccatggatgc caatctcgcc tattcatggt gtataaaagt tcaacatcca
      241 aagctagaac ttttggaaag agaaagaata tccgaatagg gcacggcgtg ccgtattgtt
      301 ggagtggact agcagaaagt gaggaaggca caggatgagt tttctcgaga cataccttca
      361 gcgtcgtctt cactgtcaca gtcaactgac agtaatcgtt gatccggaga gattcaaaat
      421 tcaatctgtt tggacctgga taagacacaa gagcgacatc ctgacatgaa cgccgtaaac
      481 agcaaatcct ggttgaacac gtatcctttt gggggcctcc cgctacgacg ctcgctccag
      541 ctggggcttc cttactatac acagcgcgca tatttcacgg ttgccagatg tcaagacaat
      601 tggtttatga aaacgtttaa tatctcgcaa caagaccttg aacttgtcga agtcgcaacg
      661 gaaaaaatta cgatgcttta cgaagacaat aaacatcatg tcggagcagc aattcgaacg
      721 aaaacgggag aaattatttc ggcagtccat attgaagcat acattggacg agtcacggtc
      781 tgcgcagaag caattgcaat tggatcggca gtctcgaatg gacaaaaaga ctttgacacg
      841 attgtcgcag tccgacatcc atactcggac gaagtcgatc gatcgattcg agtcgtctcg
      901 ccatgcggaa tgtgccgaga acttatttcg gactacgcac cagactgctt tgtccttatt
      961 gaaatgaatg gaaaacttgt caaaacgacg attgaagaac ttattccact taaatacacg
     1021 cgaaattgaa aaccaggact gaaccttcct taaaaattta attttcatta gttgcagtca
     1081 ctccgctttg gtttcacagt caggaataac actagctcgt cttcaccatg gatgccaatc
     1141 tcgcctattc atggtgtata aaagttcaac atccaaagct agaacttttg gaaagagaaa
     1201 gaatatccga atagggcacg gcgtgccgta ttgttggagt ggactagcag aaagtgagga
     1261 aggcacagga tgagttttct cgaggccggt ctccctatag tgagtcgtat taatttcgat
     1321 aagccaggtt aacctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     1381 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1441 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1501 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1561 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1621 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1681 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1741 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1801 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1861 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1921 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1981 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2041 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2101 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     2161 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2221 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2281 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     2341 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2401 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2461 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2521 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2581 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2641 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2701 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2761 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2821 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2881 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2941 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     3001 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     3061 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     3121 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     3181 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3241 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     3301 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     3361 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     3421 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     3481 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     3541 ggcatcagag cagattgtac tgagagtgca ccatatggac atattgtcgt tagaac
//
