LOCUS       sequence-242828-       11285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HA and YFP tagged Rictor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11285)
  AUTHORS   Jain A, Arauz E, Aggarwal V, Ikon N, Chen J, Ha T
  TITLE     Stoichiometry and assembly of mTOR complexes revealed by 
            single-molecule pulldown.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Dec 16;111(50):17833-8. doi: 
            10.1073/pnas.1419425111. Epub 2014 Dec 1.
  PUBMED    25453101
REFERENCE   2  (bases 1 to 11285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1419425111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-12-16- 16"; volume: "111"; issue: "50"; pages: "17833-8"
COMMENT     Sequence Label: pRK5-HA-YFP-rictor
FEATURES             Location/Qualifiers
     source          1..11285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..30
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    147..168
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(183..188,189..206,207..213)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    221..237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     226..248
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     245..261
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     245..261
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        290..669
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        670..873
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     824..844
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     870..894
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1104..1122
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1104..1121
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1135..1154
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1239..1248
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1251..1277
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      1308..1317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1314..1316,1317..1319,1320..2030)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1359..1380)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1620..1639)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1967..1988
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      7861..7870
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    7874..8008
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7911..7930)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7965..7984
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        8077..8434
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8077..8268
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8100..8120)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8285..8420
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8454..8471)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8454..8470)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8463..8485)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8683..9138
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8770..8789)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8980..9001
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9155..9174)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9274..9296
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9334..9352)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9420..9524
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9525..9593,9594..10385)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9743..9762)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10556..11144
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11045..11064
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
       61 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
      121 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      181 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      241 cacacaggaa acagctatga catgattacg aattaattcg agctcgcccg acattgatta
      301 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      361 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      421 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      481 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      541 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      601 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      661 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      721 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      781 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      841 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      901 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc
      961 ggccgggaac ggtgcattgg aacgcggatt ccccgtgcca agagtgacgt aagtaccgcc
     1021 tatagagtct ataggcccac ccccttggct tcgttagaac gcggctacaa ttaatacata
     1081 accttatgta tcatacacat acgatttagg tgacactata gaataacatc cactttgcct
     1141 ttctctccac aggtgtccac tcccaggtcc aactgcacct cggttctatc gattgaattc
     1201 ccgaaccgac agtcggtctc ttcaccaagg ccattcgcgc caccatgggc tacccctatg
     1261 atgtgcctga ctacgcagat cgcaatggtg gtggtggtgg accggtcgcc accatggtga
     1321 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1381 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1441 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1501 ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     1561 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1621 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1681 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1741 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     1801 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     1861 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1921 gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1981 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca
     2041 gatctggtgg agggcctggt ggaggtgggc ctggaggtgg aggacctggt ggtggtgggt
     2101 cgacgatggc ggcgatcggt cgtggccgct ctctgaagaa cctccgagta cgagggcgga
     2161 atgacagcgg cgaggagaac gtcccgctgg atctgacccg agaaccttct gataacttaa
     2221 gagagattct ccaaaatgtg gccagattgc agggagtatc aaatatgaga aagctaggcc
     2281 atctgaataa ctttactaag cttctttgtg atattggcca cagtgaagaa aaactgggct
     2341 ttcactatga ggatatcata atttgtttgc ggttagcttt attaaatgaa gcaaaagaag
     2401 tgcgagcagc agggctacga gcgcttcgat atctcatcca agactccagt attctccaga
     2461 aggtgctaaa attgaaagtg gactatttaa tagctaggtg cattgacata caacagagca
     2521 acgaggtaga gaggacacaa gcacttcgat tagtcagaaa gatgattact gtgaatgctt
     2581 ccttgtttcc tagttctgtg accaactcat taattgcagt tggaaatgat ggacttcaag
     2641 aaagagacag aatggtccga gcatgcattg ccattatctg tgaactagca cttcagaatc
     2701 cagaggtggt ggcccttcga ggtggactaa acaccatctt gaaaaatgtg atcgattgcc
     2761 aattaagtcg aataaatgag gccctaatta ctacaatttt gcaccttctt aatcatccaa
     2821 agactcgaca gtatgtgcga gctgatgtag aattagagag aattttagca ccctatactg
     2881 attttcacta cagacatagc ccagatacag ctgaaggaca gctcaaagaa gacagagaag
     2941 cacgatttct agccagtaaa atgggaatca tagcaacatt ccgatcatgg gcaggtatta
     3001 ttaatttatg taaacctgga aattctggga tccagtctct aataggagta ctttgcatac
     3061 caaatatgga aataaggcga ggtctacttg aagtgcttta tgatatattt cgtcttcctc
     3121 tacctgttgt gactgaggag ttcatagaag cactactcag tgtagatcca gggaggttcc
     3181 aagacagttg gaggctttca gatggctttg tggcagctga ggcaaaaact attcttcctc
     3241 atcgtgccag atccaggcca gacctcatgg ataattattt ggcactgata ctctctgcat
     3301 ttattcgtaa tggactttta gagggtctag ttgaagtgat aacaaacagt gatgatcata
     3361 tctcagttag agctaccatc cttttaggag agcttttaca tatggcaaac acaattcttc
     3421 ctcattcaca tagccatcat ttacactgct tgccaaccct aatgaatatg gctgcatcct
     3481 ttgatatccc caaggaaaag agactgcgag ccagtgcagc cttgaactgt ttaaaacgct
     3541 tccatgaaat gaagaaacga ggacctaagc cttatagtct tcatttagac cacattattc
     3601 agaaagcaat tgcaacacac cagaaacggg atcagtatct ccgagttcag aaagatatat
     3661 ttatccttaa ggatacagag gaagctcttt taattaacct tagagatagc caagtccttc
     3721 aacataaaga gaatcttgaa tggaattgga atcttatagg gaccattctt aagtggccaa
     3781 atgtaaatct aagaaactat aaagatgaac agttacacag gtttgtacga agactacttt
     3841 atttttacaa gcccagcagt aaattatatg ccaacctgga tctggatttt gccaaggcca
     3901 aacagctcac ggttgtaggt tgccagttta cagaatttct tcttgaatct gaagaggatg
     3961 ggcaaggcta cttagaagat ctagtaaagg atattgttca gtggctcaat gcttcatctg
     4021 gaatgaaacc cgaaagaagt cttcaaaata atggtttatt gaccaccctt agtcaacact
     4081 actttttatt tattggaaca ctttcttgcc accctcatgg agttaaaatg ctggaaaaat
     4141 gcagtgtatt tcagtgtctc cttaatcttt gctccttgaa aaaccaagat cacttgctaa
     4201 aacttactgt ttctagcttg gactatagca gagatggatt ggctagagtc atcctttcca
     4261 aaattttaac tgcagctact gatgcctgca gactctatgc aacaaaacat ttaagggtat
     4321 tattgagagc taatgttgaa ttctttaata attggggaat tgagttgtta gtgacccagc
     4381 tacatgataa aaacaaaacg atttcctctg aagctcttga tatcctcgat gaagcatgtg
     4441 aagacaaggc caatcttcat gctctcattc agatgaaacc agcgttatcc caccttggag
     4501 acaagggttt gcttctcctg ctgagatttc tctccattcc aaaaggattt tcctatctga
     4561 atgaaagagg ttatgtagca aaacaattgg aaaagtggca cagggaatac aactccaaat
     4621 atgttgactt gattgaggaa caactcaatg aagcacttac tacttaccgg aagcctgttg
     4681 atggtgataa ctatgttcgt cggagtaacc aaagattaca gcgtcctcac gtctacctgc
     4741 ctatacacct ttatggacaa ctagtacacc ataaaacagg ctgccatttg ttggaagtac
     4801 agaatattat tacagaactc tgtcgtaatg ttcgtacacc agatttggat aagtgggaag
     4861 aaattaaaaa actgaaagca tctctttggg ccttgggaaa tatcggctca tcaaattggg
     4921 gtctcaattt gctacaggaa gaaaacgtga ttccagatat actaaaactt gcaaaacagt
     4981 gtgaagttct ttccatcaga gggacctgtg tatatgtact tgggctcata gctaaaacca
     5041 aacaaggctg tgatattcta aaatgtcaca actgggatgc tgtgaggcat agtcgcaaac
     5101 atctgtggcc agtggttcca gatgatgtgg aacaactctg taatgaactt tcatctatcc
     5161 caagcactct aagtttgaac tcggagtcaa ccagctctag acataatagt gaaagtgaat
     5221 ctgtgccatc gagtatgttc atattggagg atgaccggtt tggcagcagc tctactagta
     5281 catttttcct tgatatcaat gaagatacag agccaacatt ttatgaccga tctggaccca
     5341 taaaggataa aaattcattc cctttctttg cttctagtaa acttgtgaag aatcgtatct
     5401 taaattcgct tactttgcct aacaaaaaac atcgtagtag cagtgatcca aaaggaggga
     5461 aattatcatc tgaaagtaag acaagcaaca ggcgaatcag aacacttacg gagcccagtg
     5521 ttgattttaa tcatagtgat gattttacac ccatatccac tgtacagaaa acattacaat
     5581 tagagacttc atttatgggg aataagcaca ttgaagacac tggtagtaca ccaagcattg
     5641 gagaaaatga cttaaaattc accaagaatt ttggtacaga gaatcacaga gaaaatacaa
     5701 gccgagagag gttagtagta gaaagttcaa cgagctcaca tatgaagata cgtagccaaa
     5761 gtttcaatac agacactaca acaagtggca taagttcaat gagctcaagt ccttcacgag
     5821 agacagtagg tgtagatgct acaactatgg acacagactg tggaagcatg agtactgtgg
     5881 taagtactaa aactattaag acaagccact atttgacgcc acagtctaac catctgtctc
     5941 tctccaaatc aaattcggtg tccctggtgc ctccaggttc ttctcatacg cttcctagaa
     6001 gagcacagtc ccttaaagca ccctctattg ctacaattaa aagtctagca gattgtaact
     6061 ttagttacac aagttctaga gatgcttttg gctatgctac actgaaaaga ctacagcaac
     6121 aaagaatgca tccatcctta tctcactctg aagctttggc atctccagca aaagatgtgc
     6181 tatttactga taccatcacc atgaaggcca acagttttga gtccagatta acaccaagca
     6241 ggttcatgaa agccttaagt tatgcatcat tagataaaga agatttattg agtcctatta
     6301 atcaaaatac cctgcaacga tcttcctcag tgcggtccat ggtgtccagt gccacatatg
     6361 ggggttcaga tgattacatt ggtcttgctc tcccggtgga tataaatgat atattccagg
     6421 taaaggatat tccctatttt cagacaaaaa acataccacc acatgatgat cgaggtgcaa
     6481 gagcatttgc ccatgatgca ggaggtcttc catctggaac tggaggtctt gtaaaaaatt
     6541 cttttcactt gctacgacag cagatgagtc ttacggaaat aatgaattca atccattcag
     6601 atgcctctct gtttttagaa agtacagaag acactggact acaggaacat acagatgata
     6661 actgccttta ttgtgtctgt attgaaattc tgggtttcca gcccagcaac caactgagtg
     6721 caatatgtag tcattcagac tttcaagata ttccatattc tgattggtgt gagcagacta
     6781 tccataatcc tttagaagtg gttccctcta agttttcggg gatttctgga tgcagtgatg
     6841 gggtgtctca agaaggctca gctagcagca ccaaaagcac agaattgtta ctaggtgtta
     6901 aaacaattcc agatgataca ccaatgtgcc gtatactcct tcgcaaagaa gttctaagat
     6961 tagtcattaa tttgagtagt tcagtttcaa ctaaatgtca tgagactggg cttttaacaa
     7021 ttaaggagaa gtatcctcaa acatttgatg acatatgcct ttactctgag gtttcccatt
     7081 tgctgtcaca ctgcacattc agacttccgt gtcggaggtt catacaagaa ttatttcaag
     7141 atgtacagtt tctacaaatg catgaagaag cagaggctgt gttggcaaca ccaccaaagc
     7201 aacctatagt tgatacatct gctgaatcct gacctcatat ttatgatgga tatagataca
     7261 tactatatat attcatattt gtggatttcc taaaagcctc agaaaatacg actgactagg
     7321 cagcaaagac aggagtatct tctgtacact gttccgcagt tactggtaca tgaacagttg
     7381 gaactgctga ctttcctaac caaaacaact tccttctctc ctttgttgag ccttttgagg
     7441 ggttcatgat tcattaccac agttttaaga gtttcagtta ccattgtatg caagagccaa
     7501 gcactgaata cctacatagg ttttctattt tctttcattt taaaagcgta atgacagtgg
     7561 aacaataatg ggatatgcag aagcaccctt cacaagttat ttctgaatga tttttagggt
     7621 aaataataca gatgccttgt ttgttaacta acttgtggaa agcaggaatc agtgtctcta
     7681 aggctgcatc ctattaccac aatggggtgt gctataactg ctggtattag agagggaact
     7741 ttggcccttt cacgtttttc ttaatgtttg taacactact tcagaggttt ataacctcaa
     7801 agcagaagaa gagcctcaac aacccgggcg gccgctaagt aagtaaggat ccccagcttg
     7861 gccgccatgg cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7921 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7981 catcaatgta tcttatcatg tctggatcgg gaattaattc ggcgcagcac catggcctga
     8041 aataacctct gaaagaggaa cttggttagg taccttctga ggcggaaaga accagctgtg
     8101 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8161 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     8221 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     8281 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8341 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     8401 aggaggcttt tttggaggcc taggcttttg caaaaagctg ttaacagctt ggcactggcc
     8461 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     8521 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     8581 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
     8641 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     8701 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     8761 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     8821 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     8881 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     8941 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     9001 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     9061 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     9121 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     9181 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     9241 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     9301 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     9361 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     9421 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     9481 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     9541 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     9601 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     9661 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     9721 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     9781 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     9841 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     9901 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     9961 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    10021 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    10081 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    10141 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    10201 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    10261 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    10321 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    10381 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    10441 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    10501 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    10561 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    10621 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    10681 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    10741 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    10801 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    10861 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    10921 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    10981 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    11041 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    11101 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    11161 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    11221 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    11281 gccga
//
