LOCUS       sequence-242831-        6714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 6714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-ERBB4
FEATURES             Location/Qualifiers
     source          1..6714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(82..109)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(201..287)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     336..358
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     350..367
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     351..367
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    383..482
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4407..4506)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4523..4542)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4524..4542)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4547..4563)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4547..4563)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4994..5785
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5878..6466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6367..6386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6620..6637
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca
       61 ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga
      121 tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg
      181 ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag
      241 ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt
      301 ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac
      361 ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag tgacctgttc
      421 gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca aaaaagttgg
      481 caccatgaag ccggcgacag gactttgggt ctgggtgagc cttctcgtgg cggcggggac
      541 cgtccagccc agcgattctc agtcagtgtg tgcaggaacg gagaataaac tgagctctct
      601 ctctgacctg gaacagcagt accgagcctt gcgcaagtac tatgaaaact gtgaggttgt
      661 catgggcaac ctggagataa ccagcattga gcacaaccgg gacctctcct tcctgcggtc
      721 tgttcgagaa gtcacaggct acgtgttagt ggctcttaat cagtttcgtt acctgcctct
      781 ggagaattta cgcattattc gtgggacaaa actttatgag gatcgatatg ccttggcaat
      841 atttttaaac tacagaaaag atggaaactt tggacttcaa gaacttggat taaagaactt
      901 gacagaaatc ctaaatggtg gagtctatgt agaccagaac aaattccttt gttatgcaga
      961 caccattcat tggcaagata ttgttcggaa cccatggcct tccaacttga ctcttgtgtc
     1021 aacaaatggt agttcaggat gtggacgttg ccataagtcc tgtactggcc gttgctgggg
     1081 acccacagaa aatcattgcc agactttgac aaggacggtg tgtgcagaac aatgtgacgg
     1141 cagatgctac ggaccttacg tcagtgactg ctgccatcga gaatgtgctg gaggctgctc
     1201 aggacctaag gacacagact gctttgcctg catgaatttc aatgacagtg gagcatgtgt
     1261 tactcagtgt ccccaaacct ttgtctacaa tccaaccacc tttcaactgg agcacaattt
     1321 caatgcaaag tacacatatg gagcattctg tgtcaagaaa tgtccacata actttgtggt
     1381 agattccagt tcttgtgtgc gtgcctgccc tagttccaag atggaagtag aagaaaatgg
     1441 gattaaaatg tgtaaacctt gcactgacat ttgcccaaaa gcttgtgatg gcattggcac
     1501 aggatcattg atgtcagctc agactgtgga ttccagtaac attgacaaat tcataaactg
     1561 taccaagatc aatgggaatt tgatctttct agtcactggt attcatgggg acccttacaa
     1621 tgcaattgaa gccatagacc cagagaaact gaacgtcttt cggacagtca gagagataac
     1681 aggtttcctg aacatacagt catggccacc aaacatgact gacttcagtg ttttttctaa
     1741 cctggtgacc attggtggaa gagtactcta tagtggcctg tccttgctta tcctcaagca
     1801 acagggcatc acctctctac agttccagtc cctgaaggaa atcagcgcag gaaacatcta
     1861 tattactgac aacagcaacc tgtgttatta tcataccatt aactggacaa cactcttcag
     1921 cacaatcaac cagagaatag taatccggga caacagaaaa gctgaaaatt gtactgctga
     1981 aggaatggtg tgcaaccatc tgtgttccag tgatggctgt tggggacctg ggccagacca
     2041 atgtctgtcg tgtcgccgct tcagtagagg aaggatctgc atagagtctt gtaacctcta
     2101 tgatggtgaa tttcgggagt ttgagaatgg ctccatctgt gtggagtgtg acccccagtg
     2161 tgagaagatg gaagatggcc tcctcacatg ccatggaccg ggtcctgaca actgtacaaa
     2221 gtgctctcat tttaaagatg gcccaaactg tgtggaaaaa tgtccagatg gcttacaggg
     2281 ggcaaacagt ttcattttca agtatgctga tccagatcgg gagtgccacc catgccatcc
     2341 aaactgcacc caagggtgta acggtcccac tagtcatgac tgcatttact acccatggac
     2401 gggccattcc actttaccac aacatgctag aactcccctg attgcagctg gagtaattgg
     2461 tgggctcttc attctggtca ttgtgggtct gacatttgct gtttatgtta gaaggaagag
     2521 catcaaaaag aaaagagcct tgagaagatt cttggaaaca gagttggtgg aaccattaac
     2581 tcccagtggc acagcaccca atcaagctca acttcgtatt ttgaaagaaa ctgagctgaa
     2641 gagggtaaaa gtccttggct caggtgcttt tggaacggtt tataaaggta tttgggtacc
     2701 tgaaggagaa actgtgaaga ttcctgtggc tattaagatt cttaatgaga caactggtcc
     2761 caaggcaaat gtggagttca tggatgaagc tctgatcatg gcaagtatgg atcatccaca
     2821 cctagtccgg ttgctgggtg tgtgtctgag cccaaccatc cagctggtta ctcaacttat
     2881 gccccatggc tgcctgttgg agtatgtcca cgagcacaag gataacattg gatcacaact
     2941 gctgcttaac tggtgtgtcc agatagctaa gggaatgatg tacctggaag aaagacgact
     3001 cgttcatcgg gatttggcag cccgtaatgt cttagtgaaa tctccaaacc atgtgaaaat
     3061 cacagatttt gggctagcca gactcttgga aggagatgaa aaagagtaca atgctgatgg
     3121 aggaaagatg ccaattaaat ggatggctct ggagtgtata cattacagga aattcaccca
     3181 tcagagtgac gtttggagct atggagttac tatatgggaa ctgatgacct ttggaggaaa
     3241 accctatgat ggaattccaa cgcgagaaat ccctgattta ttagagaaag gagaacgttt
     3301 gcctcagcct cccatctgca ctattgacgt ttacatggtc atggtcaaat gttggatgat
     3361 tgatgctgac agtagaccta aatttaagga actggctgct gagttttcaa ggatggctcg
     3421 agaccctcaa agatacctag ttattcaggg tgatgatcgt atgaagcttc ccagtccaaa
     3481 tgacagcaag ttctttcaga atctcttgga tgaagaggat ttggaagata tgatggatgc
     3541 tgaggagtac ttggtccctc aggctttcaa catcccacct cccatctata cttccagagc
     3601 aagaattgac tcgaatagga gtgaaattgg acacagccct cctcctgcct acacccccat
     3661 gtcaggaaac cagtttgtat accgagatgg aggttttgct gctgaacaag gagtgtctgt
     3721 gccctacaga gccccaacta gcacaattcc agaagctcct gtggcacagg gtgctactgc
     3781 tgagattttt gatgactcct gctgtaatgg caccctacgc aagccagtgg caccccatgt
     3841 ccaagaggac agtagcaccc agaggtacag tgctgacccc accgtgtttg ccccagaacg
     3901 gagcccacga ggagagctgg atgaggaagg ttacatgact cctatgcgag acaaacccaa
     3961 acaagaatac ctgaatccag tggaggagaa cccttttgtt tctcggagaa aaaatggaga
     4021 ccttcaagca ttggataatc ccgaatatca caatgcatcc aatggtccac ccaaggccga
     4081 ggatgagtat gtgaatgagc cactgtacct caacaccttt gccaacacct tgggaaaagc
     4141 tgagtacctg aagaacaaca tactgtcaat gccagagaag gccaagaaag cgtttgacaa
     4201 ccctgactac tggaaccaca gcctgccacc tcggagcacc cttcagcacc cagactacct
     4261 gcaggagtac agcacaaaat atttttataa acagaatggg cggatccggc ctattgtggc
     4321 agagaatcct gaatacctct ctgagttctc cctgaagcca ggcactgtgc tgccgcctcc
     4381 accttacaga caccggaata ctgtggtgcc aactttcttg tacaaagttg gcattataag
     4441 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     4501 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     4561 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     4621 atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc tgctacccaa
     4681 ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc
     4741 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
     4801 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     4861 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     4921 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     4981 aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     5041 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     5101 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     5161 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     5221 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     5281 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     5341 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     5401 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     5461 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     5521 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     5581 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     5641 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     5701 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     5761 gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat cccttaacgt
     5821 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5881 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5941 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6001 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6061 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6121 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6181 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6241 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6301 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6361 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6421 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6481 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6541 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6601 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     6661 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acag
//
