LOCUS       sequence-243098-       11702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3CB N553S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11702)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CB-N553S
FEATURES             Location/Qualifiers
     source          1..11702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             3656..3664
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(4963..4974)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6662..6686)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6694..6712
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6737..6756)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6738..6756)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6762..6782)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6836..6877
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6898..7465
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6915..7467
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7059..7076)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7286..7305
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7479..8078
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7479..7498)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7975..7995
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8092..8680
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8145..8165)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8563..8574)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8755..8988
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9107..9125)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9193..9297
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9298..9366,9367..10158)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9516..9535)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10329..10917
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10818..10837
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11071..11088
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11328..11349
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11364..11369,11370..11387,11388..11394)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11402..11418
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11407..11429
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11426..11442
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11426..11442
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11492..11511
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg tgcttcagtt tcataatgcc tcctgctatg
     3481 gcagacatcc ttgacatctg ggcggtggat tcacagatag catctgatgg ctccatacct
     3541 gtggatttcc ttttgcccac tgggatttat atccagttgg aggtacctcg ggaagctacc
     3601 atttcttata ttaagcagat gttatggaag caagttcaca attacccaat gttcaacctc
     3661 cttatggata ttgactccta tatgtttgca tgtgtgaatc agactgctgt atatgaggag
     3721 cttgaagatg aaacacgaag actctgtgat gtcagacctt ttcttccagt tctcaaatta
     3781 gtgacaagaa gttgtgaccc aggggaaaaa ttagactcaa aaattggagt ccttatagga
     3841 aaaggtctgc atgaatttga ttccttgaag gatcctgaag taaatgaatt tcgaagaaaa
     3901 atgcgcaaat tcagcgagga aaaaatcctg tcacttgtgg gattgtcttg gatggactgg
     3961 ctaaaacaaa catatccacc agagcatgaa ccatccatcc ctgaaaactt agaagataaa
     4021 ctttatgggg gaaagctcat cgtagctgtt cattttgaaa actgccagga cgtgtttagc
     4081 tttcaagtgt ctcctaatat gaatcctatc aaagtaaatg aattggcaat ccaaaaacgt
     4141 ttgactattc atgggaagga agatgaagtt agcccctatg attatgtgtt gcaagtcagc
     4201 gggagagtag aatatgtttt tggtgatcat ccactaattc agttccagta tatccggaac
     4261 tgtgtgatga acagagccct gccccatttt atacttgtgg aatgctgcaa gatcaagaaa
     4321 atgtatgaac aagaaatgat tgccatagag gctgccataa atcgaaattc atctaatctt
     4381 cctcttccat taccaccaaa gaaaacacga attatttctc atgtttggga aaataacaac
     4441 cctttccaaa ttgtcttggt taagggaaat aaacttaaca cagaggaaac tgtaaaagtt
     4501 catgtcaggg ctggtctttt tcatggtact gagctcctgt gtaaaaccat cgtaagctca
     4561 gaggtatcag ggaaaaatga tcatatttgg aatgaaccac tggaatttga tattaatatt
     4621 tgtgacttac caagaatggc tcgattatgt tttgctgttt atgcagtttt ggataaagta
     4681 aaaacgaaga aatcaacgaa aactattaat ccctctaaat atcagaccat caggaaagct
     4741 ggaaaagtgc attatcctgt agcgtgggta aatacgatgg tttttgactt taaaggacaa
     4801 ttgagaactg gagacataat attacacagc tggtcttcat ttcctgatga actcgaagaa
     4861 atgttgaatc caatgggaac tgttcaaaca aatccatata ctgaaaatgc aacagctttg
     4921 catgttaaat ttccagagaa taaaaaacaa ccttattatt accctccctt cgataagatt
     4981 attgaaaagg cagctgagat tgcaagcagt gatagtgcta atgtgtcaag tcgaggtgga
     5041 aaaaagtttc ttcctgtatt gaaagaaatc ttggacaggg atcccttgtc tcaactgtgt
     5101 gaaagtgaaa tggatcttat ttggactttg cgacaagact gccgagagat tttcccacaa
     5161 tcactgccaa aattactgct gtcaatcaag tggaataaac ttgaggatgt tgctcagctt
     5221 caggcgctgc ttcagatttg gcctaaactg cccccccggg aggccctaga gcttctggat
     5281 ttcaactatc cagaccagta cgttcgagaa tatgctgtag gctgcctgcg acagatgagt
     5341 gatgaagaac tttctcaata tcttttacaa ctggtgcaag tgttaaaata tgagcctttt
     5401 cttgattgtg ccctctctag attcctatta gaaagagcac ttggtaatcg gaggataggg
     5461 cagtttctat tttggcatct taggtcagaa gtgcacattc ctgctgtctc agtacaattt
     5521 ggtgtcatcc ttgaagcata ctgccgggga agtgtggggc acatgaaagt gctttctaag
     5581 caggttgaag cactcaataa gttaaaaact ttaaatagtt taatcaaact gaatgccgtg
     5641 aagttaaaca gagccaaagg gaaggaggcc atgcatacct gtttaaaaca gagtgcttac
     5701 cgggaagccc tctctgacct gcagtcaccc ctgaacccat gtgttatcct ctcagaactc
     5761 tatgttgaaa agtgcaaata catggattcc aaaatgaagc ctttgtggct ggtatacaat
     5821 aacaaggtat ttggtgagga ttcagttgga gtgattttta aaaatggtga tgatttacga
     5881 caggatatgt tgacactcca aatgttgcgc ttgatggatt tactctggaa agaagctggt
     5941 ttggatcttc ggatgttgcc ttatggctgt ttagcaacag gagatcgctc tggcctcatt
     6001 gaagttgtga gcacctctga aacaattgct gacattcagc tgaacagtag caatgtggct
     6061 gctgcagcag ccttcaacaa agatgccctt ctgaactggc ttaaagaata caactctggg
     6121 gatgacctgg accgagccat tgaggaattt acactgtcct gtgctggcta ctgtgtagct
     6181 tcttatgtcc ttgggattgg tgacagacat agtgacaaca tcatggtcaa aaaaactggc
     6241 cagctcttcc acattgactt tggacatatt cttggaaatt tcaaatctaa gtttggcatt
     6301 aaaagggagc gagtgccttt tattcttacc tatgatttca tccatgtcat tcaacaagga
     6361 aaaacaggaa atacagaaaa gtttggccgg ttccgccagt gttgtgagga tgcatatctg
     6421 attttacgac ggcatgggaa tctcttcatc actctctttg cgctgatgtt gactgcaggg
     6481 cttcctgaac tcacatcagt caaagatata cagtatctta aggactctct tgcattaggg
     6541 aagagtgaag aagaagcact caaacagttt aagcaaaaat ttgatgaggc gctcagggaa
     6601 agctggacta ctaaagtgaa ctggatggcc cacacagttc ggaaagacta cagatcttag
     6661 aacccagctt tcttgtacaa agtggtgata tccaattaac cctcactaaa gggtgtgtta
     6721 tttgtttgta aagtatccct atagtgagtc gtattaccac ccaacttttc tatacaaagt
     6781 ggttgatatc cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa
     6841 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgagatcc
     6901 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     6961 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     7021 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     7081 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     7141 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     7201 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     7261 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     7321 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     7381 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     7441 tttcctttga aaaacacgat gataatatgg ccacacatat gaccgagtac aagcccacgg
     7501 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     7561 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     7621 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     7681 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     7741 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     7801 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     7861 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     7921 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     7981 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     8041 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctaaat cgatagatcc taatcaacct
     8101 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     8161 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     8221 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     8281 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     8341 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     8401 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     8461 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     8521 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     8581 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     8641 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa
     8701 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     8761 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     8821 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8881 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8941 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     9001 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     9061 ccgggttaat taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc
     9121 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     9181 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     9241 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     9301 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     9361 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     9421 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     9481 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     9541 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     9601 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     9661 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     9721 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     9781 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     9841 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     9901 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     9961 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
    10021 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
    10081 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
    10141 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
    10201 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    10261 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    10321 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    10381 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    10441 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
    10501 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    10561 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    10621 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    10681 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    10741 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    10801 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    10861 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    10921 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    10981 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    11041 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
    11101 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc
    11161 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
    11221 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg
    11281 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
    11341 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
    11401 attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca
    11461 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    11521 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
    11581 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
    11641 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
    11701 gt
//
