LOCUS       sequence-243119-         534 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ddRFP-based single-color biosensor for monitoring PKA (Protein 
            Kinase A) activity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Mehta S, Zhang Y, Roth RH, Zhang JF, Mo A, Tenner B, Huganir RL, 
            Zhang J
  TITLE     Single-fluorophore biosensors for sensitive and multiplexed 
            detection of signalling activities.
  JOURNAL   Nat Cell Biol. 2018 Sep 24. pii: 10.1038/s41556-018-0200-6. doi: 
            10.1038/s41556-018-0200-6.
  PUBMED    30250062
REFERENCE   2  (bases 1 to 534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2018 Sep 24. pii: 10.1038/s41556-018-0200-6. doi: 
            10.1038/s41556-018-0200-6."
COMMENT     Sequence Label: pcDNA3-RAB-AKARev
FEATURES             Location/Qualifiers
     source          1..534
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             179..526
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
ORIGIN
        1 cactaatggg acctggttaa atgggcaaaa agtcgagaag aacagcaatc agttactgtc
       61 ccaaggtgat gaaataaccg ttggtgtagg cgtggaatca gatattttat ctctggtcat
      121 tttcataaac gacaaattta agcagtgcct ggagcagaac aaagttgatc gcggtaccag
      181 tgctggtggt agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg ctggtggttc
      241 cggcagtgct ggtggtagtg ctggtggtag taccagtgct ggtggtagtg ctggtggtag
      301 tgctggtggt agtgctggtg gtagtgctgg tggttccggc agtgctggtg gtagtgctgg
      361 tggtagtacc agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg ctggtggtag
      421 tgctggtggt tccggcagtg ctggtggtag tgctggtggt agtaccagtg ctggtggtag
      481 tgctggtggt agtgctggtg gtagtgctgg tggtagtgct ggtggttccg gatt
//
